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Technical note: Bacterial diversity and fermentation end products in rumen fluid samples collected via oral lavage or rumen cannula

New Mexico State University, Las Cruces, New Mexico, 88003

sivey{at}nmsu.edu

Abstract

A study was conducted to determine if sampling rumen contents via a ruminal cannula or oral lavage tube would yield similar denaturing gradient gel electrophoresis (DGGE) profiles of the bacterial community. Two species of ruminally cannulated animals were used for this study (cattle n = 2; sheep n = 3). All animals were allowed ad libitum access to feed. Cattle were fed baled unprocessed sorghum-sudan hay (12% CP, 68% NDF; DM basis), whereas sheep were maintained on chopped alfalfa (18% CP, 40% NDF; DM basis). Ruminal fluid was collected (approximately 20 mL) once a week for 3 wk from each animal using a poly tube equipped with a suction strainer with a handheld suction pump through the rumen cannula or oral cavity. The DGGE analysis demonstrates that yield of bacterial diversity was not different between the 2 sampling methods (P = 0.73). When samples were grouped according to band pattern similarity, groups were most stable according to individual animal and species rather than sampling method. Total VFA and molar proportions of individual VFA did not differ by sampling method (P > 0.40). Additionally, rumen ammonia concentrations were similar for both sampling methods (19.3 vs. 19.1 mM + 8.0 for cannula vs. lavage respectively; P = 0.98). These data indicate that rumen samples collected via oral lavage or rumen cannula yield similar results. This knowledge will allow sample collection from a greater population of animals and an ability to maintain the value of research livestock that can be lost due to the surgical implantation of a ruminal cannula.

Key Words: bacterial diversity • denaturing gradient gel electrophoresis • oral lavage • rumen cannula