J. Anim Sci.
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Published online first on November 7, 2008
J. Anim Sci. 1910. doi:10.2527/jas.2008-1344
© 2008 American Society of Animal Science
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Porcine Sirt1 gene clone, expression pattern, and regulation by resveratrol

Tizhong Shan, Yizhen Wang, Ting Wu, Chenxi Liu, Jia Guo, Yanfang Zhang, Jianxin Liu and Zirong Xu

Institute of Feed Science, Zhejiang University, The Key Laboratory of Molecular Animal Nutrition, Ministry of Education, No. 164 Qiutao North Road, Hangzhou 310029, P. R. China

yzwang{at}zju.edu.cn

Abstract

Sirtuin1 (Sirt1) is a nicotinamide adenine dinucleotide (NAD)-dependent deacetylase that plays important roles in a variety of biological processes. In the current study, we examined the tissue-specific and the different expression pattern of porcine Sirt1 and the effect of resveratrol (RES) on expression of Sirt1 in porcine adipocytes. The full-length cDNA sequence of porcine Sirt1 was 4,024 bp (accession no: EU030283), with a 2,226-bp open reading frame encoding 742-AA protein (the predicated molecular mass of 80.9 kDa, accession no. ABS29571). Comparison of the deduced AA sequence with the AA sequences of human, dog, cattle, and mouse Sirt1 showed 82% to 92% similarity, respectively. Furthermore, the porcine Sirt1 was highly expressed in porcine brain, to a lesser degree in spleen and white adipose tissue, and lowest but detectable in liver. In s.c. adipose tissue and omental adipose tissue, the porcine Sirt1 mRNA in adult pigs was higher than that of young pigs (P < 0.01). In vitro, exposure of cultured adipocytes to 40 and 80 µM RES for 24 h increased mRNA levels of porcine Sirt1 by 47.86% (P < 0.01) and 91.04% (P < 0.01), respectively. Accordingly, lipid accumulation and NEFA release were decreased (P < 0.05), respectively. When treated with RES for 48 h, the mRNA level of porcine Sirt1 were increased by 103.84% (P < 0.01) and 148.79% (P < 0.01), respectively. Lipid accumulation was decreased and the NEFA release was increased (P < 0.05), respectively. These results provide some information needed for manipulating Sirt1 expression in regulating fat deposition in pigs.

Key Words: cloning • gene expression • pig • resveratrol • Sirt1






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Copyright © 2008 by the American Society of Animal Science.