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Differentiation of bovine intramuscular and subcutaneous stromal-vascular cells exposed to dexamethasone and troglitazone

A. C. Grant^*, G. Ortiz-Colón^*, M. E. Doumit^*,, R. J. Tempelman^* and D. D. Buskirk^*

^* Departments of Animal Science Food Science and Human Nutrition, Michigan State University, East Lansing, MI 48824-1225

buskirk{at}msu.edu

Abstract

The objectives of these experiments were to compare differentiation of bovine stromal-vascular (S-V) cells isolated from i.m. and s.c. adipose tissues in response to a glucocorticoid and a peroxisome-proliferator activated receptor (PPAR) agonist. Stromal-vascular cells were isolated from i.m. and s.c. fat depots of 3 Angus steers and propagated in culture. Cells were exposed to differentiation media containing 0.25 µM dexamethasone (DEX), a glucocorticoid analog and 40 µM troglitazone (TRO), a PPAR agonist or both. Cells treated with DEX and TRO had greater (P < 0.02) glycerol-3-phosphate dehydrogenase (GPDH) activity than control cells. No interactions between DEX, TRO, and depot (P > 0.59), or depot differences (P = 0.41) in GDPH activity were found. Morphological assessment of adipogenic colonies showed that DEX induced a 1.8-fold increase in the percentage of adipogenic colonies (P = 0.03), while TRO increased the proportion of adipogenic colonies by 1.9-fold (P = 0.02) compared with those not treated with DEX or TRO, respectively. Depots had a similar percentage of adipogenic colonies (P = 0.18); however, the percentage of differentiated cells within adipogenic colonies was found to be 6.4-fold greater in s.c. isolates compared with i.m. (P < 0.001). Addition of TRO increased the proportion of differentiated cells within colonies by 10-fold compared with those of non-treated colonies (P < 0.001), whereas the percentage of differentiated cells within adipogenic colonies only tended to be increased by DEX (P = 0.10). These data indicate that bovine i.m. and s.c. S-V cells are capable of enhanced differentiation in response to DEX and TRO and these effects were additive. Most importantly, inherent differences in the capacity to differentiate exist between adipogenic bovine i.m. and s.c. S-V cells.

Key Words: bovine • differentiation • glycerol-3-phosphate dehydrogenase • glucocorticoid • peroxisome-proliferator activated receptor gamma • stromal-vascular

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				G. Ortiz-Colon, A. C. Grant, M. E. Doumit, and D. D. Buskirk Bovine intramuscular, subcutaneous, and perirenal stromal-vascular cells express similar glucocorticoid receptor isoforms, but exhibit different adipogenic capacity J Anim Sci, June 1, 2009; 87(6): 1913 - 1920. [Abstract] [Full Text] [PDF]