J. Anim Sci.
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Published online first on September 26, 2008
J. Anim Sci. 1910. doi:10.2527/jas.2007-0789
© 2008 American Society of Animal Science
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Detection of BoLA-DRB3 alleles as candidate markers for clinical mastitis resistance in Holstein x Zebu

M. Duangjinda*, D. Buayai* , V. Pattarajinda*, Y. Phasuk*, S. Katawatin*, T. Vongpralub* and A. Chaiyotwittayakul{dagger}

* Department of Animal Science, Faculty of Agriculture {dagger} Department of Medicine, Faculty of Veterinary Medicine, Khon Kaen University, Thailand 40002

monchai{at}kku.ac.th

Abstract

BoLA-DRB3 alleles from Holstein x Zebu crossbred dairy cows (n = 409) were analyzed using the PCR-RFLP technique. Exon II of DRB3 was amplified using locus specific primers (HLO30/HLO32), followed by digestion with 3 restriction enzymes (RsaI, BstyI, and HaeIII). Forty alleles were found with frequency ranging from 0.005 to 0.139. The most frequently detected alleles of Holstein x Zebu were DRB3*16, *51, *23, *11, *8, and *1 accounting for 61.12% of the alleles in the population. Detection of candidate alleles for clinical mastitis occurrence was performed by logistic regression. It was found that percent of Holstein fraction in crossbred cows had a non-significant effect (P > 0.05). However, parity had a significant effect on mastitis occurrence. In addition, DRB3*1 and *52 were the most associated with the occurrence of clinical mastitis, whereas *15, *51, and *22 were associated with resistance in crossbred populations. This is the first report of association of DRB3*15 and *51 with mastitis resistance. The association was validated by examining the candidate alleles in another commercial population. Highly susceptible (n = 43) and resistant (n = 42) groups of Holstein x Zebu cows were investigated. The result confirmed that DRB3*1 and *52 could be considered as susceptibility alleles, whereas *15, *51, and *22 could be considered as resistant alleles in Holstein x Zebu raised under tropical conditions. In addition, allele effects on 305-d milk production were estimated by Best Linear Unbiased Prediction (BLUP). It was shown that most alleles associated with high clinical mastitis occurrence were related to high milk yield. This study revealed that allele DRB3*10 had the highest effect on increasing milk yield with moderate resistance to clinical mastitis, which could be used as a potential marker for selection in dairy genetic evaluation.

Key Words: BoLA-DRB3 • candidate marker • clinical mastitis • Holstein x Zebu






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