HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) All Versions of this Article: jas.2007-0708v1 86/11/3045    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lourenço, M. Articles by Fievez, V. Search for Related Content PubMed PubMed Citation Articles by Lourenço, M. Articles by Fievez, V.

Effects of saponins, quercetin, eugenol, and cinnamaldehyde on fatty acid biohydrogenation of forage polyunsaturated fatty acids in dual-flow continuous culture fermenters

M. Lourenço^*, P. W. Cardozo, S. Calsamiglia and V. Fievez^*

^* Laboratory for Animal Production and Animal Product Quality, Department of Animal Production, Ghent University, Proefhoevestraat 10, 9090 Melle, Belgium Animal Nutrition, Management, and Welfare Research Group, Department de Ciència Animal i dels Aliments, Universitat Autònoma de Barcelona, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain

Veerle.Fievez{at}UGent.be

Abstract

Four different plant secondary metabolites were screened for their effect on rumen biohydrogenation of forage long chain fatty acids, using dual-flow continuous culture fermenters. Treatments were: control (no additive), positive control (12 mg/L of monensin), and plant extracts (500 and 1,000 mg/L triterpene saponin; 250 and 500 mg/L quercetin; 250 mg/L eugenol; 500 mg/L cinnamaldehyde). Monensin increased propionate, decreased acetate and butyrate proportions, and inhibited the complete biohydrogenation of fatty acids resulting in the accumulation of intermediates of the biohydrogenation process (C18:2 trans-11, cis-15 rather than C18:1 trans-11). Cinnamaldehyde reduced total VFA concentration and proportions of odd and branched chain fatty acids in total fat effluent. Apparent biohydrogenation of C18:2n-6 and C18:3n-3 was also lower, and a shift from the major known biohydrogenation pathway to a secondary pathway of C18:2n-6 was observed, as evidenced by an accumulation of C18:1 trans-10 and trans-10, cis-12 CLA. Quercetin (500 mg/L) increased total VFA concentration, but no shifts in the pathways or extent of biohydrogenation were observed. Eugenol resulted in the accumulation of C18:1 trans-15 and C18:1 cis-15, end products of an alternative biohydrogenation pathway of C18:3n-3. Triterpene saponins did not affect the fermentation pattern, the biohydrogenation pathways, or the extent of biohydrogenation. At the doses tested in this study, we could only show a direct relation between changes in the rumen fatty acid metabolism and the presence of cinnamaldehyde, but not for eugenol, quercetin, or triterpene saponins.

Key Words: biohydrogenation • cinnamaldehyde • eugenol • flavonoids • in vitro • saponins

This article has been cited by other articles:

				C. Benchaar and P. Y. Chouinard Short communication: Assessment of the potential of cinnamaldehyde, condensed tannins, and saponins to modify milk fatty acid composition of dairy cows J Dairy Sci, July 1, 2009; 92(7): 3392 - 3396. [Abstract] [Full Text] [PDF]