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This Article Full Text (PDF) All Versions of this Article: jas.2007-0685v1 86/12/3279    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Bates, J. S. Articles by Johnson, R. K. Search for Related Content PubMed PubMed Citation Articles by Bates, J. S. Articles by Johnson, R. K.

Differential expression in lung and bronchial lymph node of pigs with high and low responses to infection with Porcine Reproductive and Respiratory Syndrome Virus

Department of Animal Science, University of Nebraska, Lincoln, NE 68583-0908

rjohnson5{at}unl.edu

Abstract

One hundred Hampshire by Duroc crossbred pigs (HD) and 100 NE Index line pigs (I) were infected with porcine reproductive and respiratory syndrome virus (PRRSV) and evaluated for resistance/susceptibility. Controls (100/line) were uninfected littermates to infected pigs. Viremia (V), weight change (WT), and rectal temperature at 0, 4, 7, and 14 days post-infection (dpi) were recorded. Lung, bronchial lymph node (BLN), and blood tissue were collected at necropsy (14 dpi). Infected pigs were classified as low or high responders to PRRSV based on the first principal component (PC) from principal component analyses of all variables. Low responders to PRRSV (low PRRSV burden) and their uninfected littermates were assigned to low (L) class. High responders to PRRSV (high PRRSV burden) and their uninfected littermates were assigned to high (H) class. Infected pigs in the L-class had high WT, low V, and few lung lesions; H-class pigs had low WT, high V, and many lung lesions. RNA was extracted from lung and BLN tissue of the seven highest and seven lowest responders per line and from each of their control littermates. A control reference design was used and cDNA from each reference sample tissue was prepared from pooled RNA extracted from two control pigs from each line whose infected littermates had a PC value of 0. Design variables in data analyses were line (I vs HD), class (H vs L), treatment (infected vs uninfected controls), and slide/pig as error. Oligo differential expression was based on p < 0.01 occurring in both lung and BLN. Line and treatment effects were significant for 38 and 541 oligos, respectively, in both lung and BLN. Line by class interaction existed for expression of Thymosin-beta 4, DDX3X, Acetylcholinesterase, and XIST in both tissues. Treatment by class existed for expression of CEPB, NFBI, TXNIP, MFSD1, and unknown sequences SS00012040 and SS00012343. Line by treatment and line by treatment by class interactions were not significant. Possible important genetic associations for fine mapping candidate genes related to response to PRRSV and determining causative alleles were revealed.

Key Words: Pigs • Microarray • PRRS virus resistance • Gene expression