J. Anim Sci.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Published online first on April 11, 2008
J. Anim Sci. 1910. doi:10.2527/jas.2007-0659
© 2008 American Society of Animal Science
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jas.2007-0659v1
86/8/1781    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Shan, T.
Right arrow Articles by Xu, Z.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shan, T.
Right arrow Articles by Xu, Z.
J. Anim Sci., doi: 10.2527/jas.2007-0659
©Copyright, 2008, The American Society of Animal Science

ARTICLE

Porcine adipose triglyseride lipase gene clone, expression pattern and regulation by resveratrol

T. Shan 1, Y. Wang 1*, T. Wu 1, J. Guo 1, J. Liu 1, J. Feng 1, Z. Xu 1

1 Institute of Feed Science, Zhejiang University, The Key Laboratory of Molecular Animal Nutrition, Ministry of Education, No. 164 Qiutao North Road, Hangzhou 310029, P. R. China

* To whom correspondence should be addressed. E-mail: chuxn{at}zju.edu.cn.


  Abstract

Adipose triglyceride lipase (ATGL) was recently identified and described as a major novel triglyceride lipase in animals. In this study, we aimed to study the tissue-specific and developmental expression pattern of porcine (p) ATGL, and the effect of resveratrol (RES) on expression of pATGL in vitro. The full-length cDNA sequences of pATGL was 1958 bp (accession no: EF583921), with a 1458 bp Open Reading Frame encoding a 486 AA protein (the predicated molecular mass of 53.2 kDa, accession no. ABS58651). Comparison of the deduced AA sequence with the bovine, mouse, rat, dog and human adipose triglyceride lipase showed 87%, 84%, 83%, 81% and 80% similarity, respectively. Furthermore, the pATGL was highly expressed in porcine adipose tissue, to a lesser degree in kidney, heart, and muscle, and least but detectable in brain. In s.c. adipose tissue, pATGL mRNA was very low at birth (1 kg BW), and increased, reaching a maximal value at 20 kg BW (about 8 wk old; P < 0.01). In peritoneal and omental adipose tissue, the greatest expression of pATGL was observed at 40 kg BW (about 12 wk old). In vitro, exposure of cultured adipocytes to 40 µM resveratrol and 80 µM resveratrol for 24 h increased the mRNA levels of pATGL by 95.3% (P < 0.05) and 146.8% (P < 0.01), respectly. Accordingly, lipid accumulation was decreased by 25.7% (P < 0.05) and 60.8% (P < 0.01), respectively. When treated with resveratrol for 48h, the mRNA levels of pATGL were increased by 104.1% (P < 0.05) and 163.1% (P < 0.01), respectively. As expected, lipid accumulation was decreased by 9.7% (P > 0.05) and 29.0% (P < 0.05), respectively. These results add to our understanding of the role of pATGL in adipose tissue development and as a potential target for regulating fat deposition and meat quality.

Key Words: adipose triglyceride lipase, cloning, gene expression, pig, resveratrol




This article has been cited by other articles:


Home page
 J ANIM SCIHome page
T. Shan, Y. Wang, T. Wu, C. Liu, J. Guo, Y. Zhang, J. Liu, and Z. Xu
Porcine sirtuin 1 gene clone, expression pattern, and regulation by resveratrol
J Anim Sci, March 1, 2009; 87(3): 895 - 904.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
R. Zechner, P. C. Kienesberger, G. Haemmerle, R. Zimmermann, and A. Lass
Adipose triglyceride lipase and the lipolytic catabolism of cellular fat stores
J. Lipid Res., January 1, 2009; 50(1): 3 - 21.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
M. Prentki and S. R. M. Madiraju
Glycerolipid Metabolism and Signaling in Health and Disease
Endocr. Rev., October 1, 2008; 29(6): 647 - 676.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Copyright © 2008 by the American Society of Animal Science.