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Effect of feeding high-temperature-micro-time (HTMT) treated diets with different lipid sources on conjugated linoleic acid (CLA) formation in finishing Hanwoo steers

C.X. Xu^*,, Y.K. Oh, H.G. Lee, T.G. Kim^#, Z.H. Li^*, J.L. Yin^*, Y.C. Jin^*, H. Jin, Y.J. Kim¹, K.H. Kim, J.M. Yeo^¶ and Y.J. Choi^*

^* Laboratory of Animal Cell Biotechnology, School of Agricultural Biotechnology , Laboratory of Toxicology, College of Veterinary Medicine, Seoul National University, Seoul 130-712, Korea , School of Bio-Resources, PNU-Special Animal Biotechnology Center, Pusan National University, Gyeongnam 627-706, Korea , National Institute of Animal Science, RDA, Suwon 441-706, Korea , ^# Agribrands Purina Korea Inc., Seongnam 463-808, Korea , ¹ Department of Food Science & Biotechnology, Korea University, Chochiwon, Chungnam 339-700, Korea ^¶ Korea National Agricultural College, RDA, Suwon, 441-706, Korea

cyjcow{at}snu.ac.kr

Abstract

The present study was conducted to examine effects of different plant oils or plant oil mixtures and high-temperature-micro-time processing (HTMT) on CLA content in Hanwoo steers. Experiment 1 consisting of 3 in vitro trials was conducted to determine how the biohydrogenation of C18 fatty acids and CLA production were affected by fat sources (tallow, soybean oil, linseed oil, or mixtures of soybean oil and linseed oil) or HTMT treatment in rumen fluid. The results showed that HTMT was capable of protecting unsaturated fatty acids from biohydrogenation by ruminal bacteria. The HTMT-treated diet containing 4% linseed oil (LU) and a supplement containing 2% linseed oil and 1% soybean oil treated with HTMT + 1% soybean oil (L₂S₁U+S₁) produced high levels of trans-11 C18:1, and cis-9, trans-11 CLA, and low level of trans-10, cis-12 CLA. Based on these results, in vivo studies (Exp. 2) were conducted using LU and L₂S₁U+S₁. These 2 treatments increased the content of cis-9, trans-11 CLA in LM compared with the control diet. The content of trans-10, cis-12 CLA in s.c. fat was also increased in L₂S₁U+S₁ treatment compared with other treatments. The s.c. fat thickness in LU treatment was decreased compared with L₂S₁U+S₁ treatment. The LU treatment significantly decreased fatty acid synthase (FAS) expression but simultaneously increased leptin expression. In this report, we showed that diets containing LU and L₂S₁U+S₁ were capable of increasing CLA in intramuscular fat of beef.

Key Words: High-temperature-micro-time • CLA • trans-11 C18:1 • beef