J. Anim Sci.
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Published online first on September 18, 2007
J. Anim Sci. 1990. doi:10.2527/jas.2007-0399
© 2007 American Society of Animal Science
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J. Anim Sci., doi: 10.2527/jas.2007-0399
©Copyright, 2007, The American Society of Animal Science

ARTICLE

Ectopic expression of porcine peroxisome-proliferator-activated receptor {delta} regulates adipogenesis in myoblasts

Y. H. Yu 1, S. C. Wu 1, W. T. K. Cheng 1, H. J. Mersmann 1, S. T. Ding 1*

1 Department of Animal Science and Technology/Institute of Biotechnology, National Taiwan University, Taipei 106, Taiwan

* To whom correspondence should be addressed. E-mail: sding{at}ntu.edu.tw.


  Abstract

Peroxisome-proliferator-activated receptor {gamma} (PPAR{gamma}) plays a critical role in regulating adipogenesis. The expression of PPAR{delta} precedes that of PPAR{gamma} during adipocyte differentiation in rodents. The current experiment was designed to study the function of porcine PPAR{delta} and the interaction of PPAR{delta} and PPAR{gamma} in adipocyte differentiation. Inhibition of myogenesis was observed in mouse myoblasts expressing porcine PPAR{delta}, similar to myoblasts expressing PPAR{gamma}. Treatment of myoblasts expressing PPAR{delta} with ligands for both PPAR{delta} and {gamma} enhanced lipogenesis and adipogenesis to a greater extent than treatment with a PPAR{gamma} ligand alone, suggesting that both genes were involved in regulating lipogenesis and adipogenesis. The ability to transdifferentiate myoblasts into adipocytes was decreased in myoblasts co-expressing PPAR{delta} with either wild-type or mutated PPAR{gamma} (serine 112 was mutated to alanine; the mutated PPAR{gamma} is more active than wild-type) compared with myoblasts expressing PPAR{gamma} alone. Adipocyte differentiation in myoblasts co-expressing PPAR{delta} and mutated PPAR{gamma} was greater than in myoblasts co-expressing PPAR{delta} and wild-type PPAR{gamma}, confirming that the serine 112 is important for the function of PPAR{gamma}. Taken together, our results demonstrate that overexpression of PPAR{delta} inhibits myotube formation and also enhances adipocyte differentiation. However, the complexity and interaction of PPAR{delta} and PPAR{gamma} in adipogenesis are not clearly understood.

Key Words: Adipocyte differentiation, myogenin, peroxisome proliferator-activated receptor {delta}, peroxisome proliferator-activated receptor {gamma}, pigs






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Copyright © 2007 by the American Society of Animal Science.