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This Article Full Text (PDF) All Versions of this Article: jas.2007-0082v1 86/9/2277    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by El-Kadi, S. W. Articles by Vanzant, E. S. Search for Related Content PubMed PubMed Citation Articles by El-Kadi, S. W. Articles by Vanzant, E. S.

Nutrient net absorption across the portal-drained viscera of forage-fed beef steers: Quantitative assessment and application to a nutritional prediction model

S. W. El-Kadi^*, K. R. McLeod^*, N. A. Elam^¶, S. E. Kitts^*, C. C. Taylor^*, D. L. Harmon^*, B. J. Bequette and E. S. Vanzant

^* Department of Animal and Food Sciences, University of Kentucky, Lexington 40546 , Department of Animal and Avian Sciences, University of Maryland, College Park 20742 ^¶ Clayton Livestock Research Center, 15 NMSU Lane, Clayton, NM 88415

kmcleod{at}uky.edu

Abstract

This study aimed to establish the relationship between ME intake and energy and nutrient absorption across the portal drained viscera (PDV) of forage-fed beef steers. Eight Angus (328 ± 40 kg BW) steers were surgically fitted with portal, mesenteric arterial, and mesenteric venous catheters, and were fed alfalfa cubes in a replicated 4x4 Latin square design with 4 levels of energy intake between 1 and 2 x maintenance energy requirements. On d 28 of each experimental period, p-aminohippuric acid was infused to measure blood and plasma flow across the PDV, and blood samples (1 every hour, for 6 h) were simultaneously collected from arterial and venous catheters for net absorption measurements. Oxygen utilization, and therefore energy utilization, linearly increased (P < 0.05) in relation to ME intake. Glucose net uptake was unaffected, but lactate net release linearly increased in response to ME intake (P < 0.05). Net absorption of all AA except tryptophan, glutamate, and glutamine linearly increased with ME intake (P < 0.05). The constant net absorption of glutamate and glutamine indicated an increased net utilization of these AA when dietary supply was increased. These data provide quantitative measures of the PDV effects on energy and AA availability for productive tissues, and suggest that the greater net utilization of some AA when ME intake is increased could relate to their catabolism for energy production. Prediction estimates of small intestinal AA absorption, based on the Cornell Net Carbohydrate and Protein System (CNCPS), exceeded observed net AA PDV absorption. Mean bias represented the largest proportion (87 to 96%) of the deviation between individual AA absorption and observed net AA PDV absorption, thereby suggesting that the CNCPS model may be used to predict AA net absorption when factors describing AA utilization by the PDV are applied to model predictions.

Key Words: Amino acids • Beef • Energy • Intake • Metabolism • Portal drained viscera