Plasma diamine oxidase: a biomarker of copper deficiency in the bovine

¹ Department of Animal Science and Interdepartmental Nutrition Program, North Carolina State University, Raleigh, North Carolina, 27695

^* To whom correspondence should be addressed. E-mail: Jerry_Spears{at}ncsu.edu.

	Abstract

This study was designed to test the efficacy of plasma diamine oxidase (DAO) activity as a biomarker of Cu deficiency in the bovine. Angus steers (n = 11) and heifers (n = 17) were assigned to 1 of 3 treatments: 1) control (adequate dietary Cu); 2) Cu-deficient (-Cu); and 3) Cu-deficient plus high dietary manganese (-Cu+Mn) and fed from weaning through finishing. Molybdenum (2 mg/kg DM) was supplemented to treatments -Cu and -Cu+Mn to induce Cu deficiency via the formation of ruminal thiomolybdates. Samples were collected on 2 sampling dates (d 160 and 190) to determine the efficacy of plasma DAO activity as a biomarker of Cu deficiency. For both sampling dates, liver Cu, plasma Cu, and plasma ceruloplasmin activity indicated that cattle receiving diets designed to induce Cu deficiency (-Cu and -Cu+Mn) were Cu-deficient with all indices of Cu status lower (P < 0.001) than the control animals. In addition to these traditional indices of Cu status, plasma DAO activity also effectively identified Cu-deficient animals, as plasma DAO levels were reduced (P < 0.001) 2- to 3-fold compared to controls. Correlation analysis indicated that plasma DAO activity was highly correlated to all other indices of Cu status (Pearson R = 0.73 to 0.87). During the growing phase, ADG (P = 0.09) and G:F (P = 0.002) were depressed in Cu-deficient animals compared to controls, whereas cattle performed equally well across all treatments in the finishing phase. The plasma DAO activity assay was precise and reliable based on an intraassay CV of 4.42% and interassay CV of 11.1%. Due to increased variability, freezing and thawing plasma samples resulted in significant changes in DAO activity relative to fresh plasma DAO activity values. Thus, fresh plasma DAO activity, a relatively simple assay, may serve as an effective tool to diagnose Cu deficiency in the bovine.

Key Words: Cattle, Copper deficiency, Diamine oxidase