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ARTICLE |
1 Department of Animal Science, University of Nebraska, Lincoln, NE 68583-0908
2 Animal Parasitic Diseases Laboratory ANRI, ARS, USDA Building 1040, Room 103, BARC-East Beltsville, MD 20705
* To whom correspondence should be addressed. E-mail: rjohnson5{at}unl.edu.
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Abstract |
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One hundred Hampshire by Duroc crossbred pigs (HD) and 100 NE Index line pigs (I) were infected with porcine reproductive and respiratory syndrome virus (PRRSV) and evaluated for resistance/susceptibility. Controls (100/line) were uninfected littermates to infected pigs. Viremia (V), weight change (WT
), and rectal temperature at 0, 4, 7, and 14 days post-infection (dpi) were recorded. Lung, bronchial lymph node (BLN), and blood tissue were collected at necropsy (14 dpi). The first principal component from principal component analyses of all variables was used to rank pigs for phenotypic response to PRRSV. Low (L) responders (low PRRS burden) had high WT low V, and few lung lesions; high (H) responders (high PRRS burden) had low WT high V, and many lesions. RNA was extracted from lung and BLN tissue of the seven highest and seven lowest responders per line, and from each of their littermates. Expression of 11 specific innate and T helper 1 immune markers was evaluated with cDNA in a 2x2x2 factorial design. Significant up-regulation in lung and/or lymph of infected pigs relative to controls occurred for all but one gene. Expression differences were greater in HD than I pigs. Significant down-regulation for certain immune genes in L pigs, relative to littermate controls, was detected in lung and BLN, particularly in Line I. Serum levels of the immune cytokines affirmed the gene expression differences. High pre-infection serum levels of interleukin-8 were significantly associated with PRRSV-resistant, L pigs. Following infection, low expression of interferon-gamma in cDNA and in serum was also correlated with PRRSV resistance. Important genetic associations for fine mapping candidate genes for PRRSV resistance and determining causative alleles were revealed.
Key Words: pigs, immune response, PRRS virus resistance, gene expression
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