J. Anim Sci.
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Published online first on November 22, 2006
J. Anim Sci. 1990. doi:10.2527/jas.2006-535
© 2006 American Society of Animal Science
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J. Anim Sci., doi: 10.2527/jas.2006-535
©Copyright, 2006, The American Society of Animal Science

ARTICLE

Effects of added fermentable carbohydrates in the diet on intestinal pro-inflammatory cytokine specific mRNA content in weaning piglets

S. Pié 1, A. Awati 2, S. Vida 1, I. Falluel 1, B. A. Williams 2, I. P. Oswald 1*

1 Institut National de la Recherche Agronomique, Unité de Pharmacologie-Toxicologie, UR66, 180 chemin de Tournefeuille - 31931 Toulouse Cedex 9 - France
2 Animal Nutrition Group, Wageningen University and Research Centre, P. O. Box 338, 6700 AH, Wageningen, The Netherlands

* To whom correspondence should be addressed. E-mail: ioswald{at}toulouse.inra.fr.


  Abstract

There is increasing evidence to show that dietary supplementation with prebiotics can be effective in the treatment of intestinal inflammation. As weaning time is characterized by a rapid inflammation, this study investigated the effect of a diet supplemented with a combination of 4 fermentable carbohydrates (lactulose, inulin, sugarbeet pulp, and wheat starch) on the mRNA content of pro-inflammatory cytokines in newly weaned piglets. Cytokines (IL-1{beta}, IL-6, IL-8, IL-12p40, IL-18, and TNF-{alpha}) were analyzed using an RT-PCR technique on d 1, 4, and 10 in the ileum and colon of piglets fed either test diet (CHO) or control diet (CON). In addition to the diet, the effect of enforced fasting on cytokine mRNA content was also evaluated. No effect of fasting was observed on pro-inflammatory cytokine mRNA content. Our results showed that the CHO diet induced an up-regulation of IL-6 mRNA content in the colon of piglets 4 d post-weaning. This up-regulation was specific for the animals fed with the CHO diet and was not observed in animals fed the CON diet. An increase in IL-1{beta} mRNA content was also observed on d 4 post-weaning in all the piglets. Correlation between pro-inflammatory cytokines and end-products of fermentation, indicated that the regulation of cytokines may be linked with some of the fermentation end-products such as branched-chain fatty acid, which are in turn, end-products of protein fermentation.

Key Words: Pig, Prebiotic, Pro-inflammatory cytokine, Weaning




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T. E. Weber, C. J. Ziemer, and B. J. Kerr
Effects of adding fibrous feedstuffs to the diet of young pigs on growth performance, intestinal cytokines, and circulating acute-phase proteins
J Anim Sci, April 1, 2008; 86(4): 871 - 881.
[Abstract] [Full Text] [PDF]


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