J. Anim Sci.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Published online first on February 12, 2007
J. Anim Sci. 1990. doi:10.2527/jas.2006-491
© 2007 American Society of Animal Science
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jas.2006-491v1
85/6/1511    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by O'Fallon, J. V.
Right arrow Articles by Gaskins, C. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by O'Fallon, J. V.
Right arrow Articles by Gaskins, C. T.
J. Anim Sci., doi: 10.2527/jas.2006-491
©Copyright, 2007, The American Society of Animal Science

ARTICLE

A direct method for fatty acid methyl ester (FAME) synthesis: Application to wet meat tissues, oils and feedstuffs

J. V. O'Fallon 1, J. R. Busboom 1, M. L. Nelson 1, C. T. Gaskins 1*

1 Department of Animal Sciences, Washington State University, Pullman 99164-6351

* To whom correspondence should be addressed. E-mail: gaskins{at}wsu.edu.


  Abstract

A simplified protocol to obtain fatty acid methyl esters (FAME) directly from fresh tissue, oils, or feedstuffs, without prior organic solvent extraction is presented. With this protocol, FAME synthesis is conducted in the presence of up to 33% water. Wet tissues, or other samples, are permeabilized and hydrolyzed for 1.5 hr at 55 °C in 1N KOH in methanol containing C13:0 as internal standard. The KOH is neutralized and the free fatty acids are methylated by H2SO4 catalysis for 1.5 hr at 55 °8C. Hexane is then added to the reaction tube, which is vortex-mixed and centrifuged. The hexane is pipetted into a GC vial for subsequent gas chromatography. All reactions are conducted in a single screw cap Pyrex tube for convenience. The method meets a number of criteria for fatty acid analysis including not isomerizing conjugated linoleic acid (CLA) or introducing fatty acid artifacts. It is applicable to fresh, frozen, or lyopholyzed tissue samples, in addition to oils, waxes and feedstuffs. The method saves time, effort and is economical when compared to other methods. Its unique performance, including easy sample preparation, is achieved because water is included in the FAME reaction mixtures rather than eliminated.

Key Words: fatty acid analysis, FAME synthesis, longissimus muscle, conjugated linoleic acid (CLA), fish oil, feedstuffs




This article has been cited by other articles:


Home page
 J ANIM SCIHome page
L. Eastwood, P. R. Kish, A. D. Beaulieu, and P. Leterme
Nutritional value of flaxseed meal for swine and its effects on the fatty acid profile of the carcass
J Anim Sci, November 1, 2009; 87(11): 3607 - 3619.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
Z. Gao, J. Yin, J. Zhang, R. E. Ward, R. J. Martin, M. Lefevre, W. T. Cefalu, and J. Ye
Butyrate Improves Insulin Sensitivity and Increases Energy Expenditure in Mice
Diabetes, July 1, 2009; 58(7): 1509 - 1517.
[Abstract] [Full Text] [PDF]

Home page
 J ANIM SCIHome page
M. L. Nelson, J. R. Busboom, C. F. Ross, and J. V. O'Fallon
Effects of supplemental fat on growth performance and quality of beef from steers fed corn finishing diets
J Anim Sci, April 1, 2008; 86(4): 936 - 948.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Copyright © 2007 by the American Society of Animal Science.