J. Anim Sci.
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Published online first on December 4, 2006
J. Anim Sci. 1990. doi:10.2527/jas.2006-385
© 2006 American Society of Animal Science
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J. Anim Sci., doi: 10.2527/jas.2006-385
©Copyright, 2006, The American Society of Animal Science

ARTICLE

Fatty acid composition of ruminal bacteria and protozoa with emphasis on conjugated linoleic acid, vaccenic acid, and odd-chain and branched-chain fatty acids

M. M. Or-Rashid 1, N. E. Odongo 1, B. W. McBride 1*

1 Department of Animal and Poultry Science, University of Guelph, Guelph, ON, Canada N1G 2W1

* To whom correspondence should be addressed. E-mail: bmcbride{at}uoguelph.ca.


  Abstract

Knowledge of the fatty acid profile of microbial lipid is of great nutritional importance to the animal and subsequently their products. This study was conducted to examine the fatty acid profiles of mixed rumen bacteria and protozoa. Bacterial and protozoal cells were isolated by differential centrifugation of rumen contents. The main fatty acids were palmitic (16:0) and stearic (18:0) in both the bacterial and protozoal fractions. Palmitic acid was 74% higher in the protozoal fatty acids than in the bacterial fatty acids, whereas bacteria had 2.25 times higher stearic acid (18:0) proportions compared to protozoa. The total odd-chain plus branched-chain fatty acids were 16.5% of bacterial fatty acids and 11.0% of protozoal fatty acids. The anteiso-17:0 proportions in bacterial and protozoal fatty acids were 1.4% and 2.9%, respectively. The most abundant trans-18:1 isomer, vaccenic acid (18:1 trans-11), was 6.6% of total fatty acids in protozoa and 2.0% of total fatty acids in bacteria. The cis-9, trans-11 CLA was 8.6 times higher in the protozoal fraction (1.32% of total fatty acids) than in the bacterial fraction (0.15%). These results suggest that the presence of protozoa in the rumen may increase the supply of CLA and other unsaturated fatty acids for lower gut absorption by ruminants.

Key Words: CLA, odd- and branched-chain fatty acid, rumen bacteria and protozoa, vaccenic acid




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