J. Anim Sci.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Published online first on October 23, 2006
J. Anim Sci. 1990. doi:10.2527/jas.2006-366
© 2006 American Society of Animal Science
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jas.2006-366v1
85/3/571    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cue, R. A.
Right arrow Articles by Doran, O.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cue, R. A.
Right arrow Articles by Doran, O.
J. Anim Sci., doi: 10.2527/jas.2006-366
©Copyright, 2006, The American Society of Animal Science

ARTICLE

Breed-associated variations in the sequence of the pig 3{beta}-hydroxysteroid dehydrogenase gene

R. A. Cue 1, S. I. Nicolau-Solano 1, J. D. McGivan 1, J. D. Wood 1, O. Doran 1*

1 Division of Farm Animal Science, School of Clinical Veterinary Science, University of Bristol, Langford, Bristol, BS40 5DU, UK.

* To whom correspondence should be addressed. E-mail: e.udovikova{at}bristol.ac.uk.


  Abstract

The entire sequence of the pig 3{beta}-hydroxysteroid dehydrogenase (3{beta}-HSD) gene has recently become known. This gene is deemed to be important in androstenone metabolism in pig liver and its defective expression has been shown to be related to androstenone accumulation in adipose tissue and the development of boar taint. The aim of the present work was (i) to define the structure of the pig 3{beta}-HSD gene and (ii) to compare 3{beta}-HSD DNA sequences from pigs of different breeds, which vary in adipose tissue androstenone levels in order to identify a polymorphism which might be responsible for differential 3{beta}-HSD expression. The 5' flanking and the coding region of 3{beta}-HSD were cloned and sequenced by conventional techniques. The 3{beta}-HSD coding regions were identical in pigs of different breeds and in animals with high and low androstenone levels. Significant sequence variations were found in the 5' flanking region of the 3{beta}-HSD gene, where differences in the number of TTAT repeats and 3 SNP were observed. The SNP were associated with the number of the TTAT repeats. These variations in the 3{beta}-HSD DNA sequence were not associated with androstenone level in subcutaneous adipose tissue but were breed-dependent. The results of this work might be used for detection of the presence of Meishan genes in Western pig breeds especially if phenotype is not clearly established.

Key Words: breed, pig, 3-beta-hydroxysteroid dehydrogenase, polymorphism




This article has been cited by other articles:


Home page
 J ANIM SCIHome page
G. Chen, E. Bourneuf, S. Marklund, G. Zamaratskaia, A. Madej, and K. Lundstrom
Gene expression of 3{beta}-hydroxysteroid dehydrogenase and 17{beta}-hydroxysteroid dehydrogenase in relation to androstenone, testosterone, and estrone sulphate in gonadally intact male and castrated pigs
J Anim Sci, October 1, 2007; 85(10): 2457 - 2463.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Copyright © 2006 by the American Society of Animal Science.