J. Anim Sci.
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Published online first on December 18, 2006
J. Anim Sci. 1990. doi:10.2527/jas.2006-278
© 2006 American Society of Animal Science
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J. Anim Sci., doi: 10.2527/jas.2006-278
©Copyright, 2006, The American Society of Animal Science

ARTICLE

Imprinted status of pleiomorphic adenoma gene-like I and paternal expression gene 10 genes in pigs

F. W. Zhang 1, H. C. Cheng 1, C. D. Jiang 2, C. Y. Deng 1*, Y. Z. Xiong 1, F. E. Li 1, M. G. Lei 1

1 Key Laboratory of Pig Genetics and Breeding, Ministry of Agriculture & Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, China
2 Department of Bio-engineering, College of Animal Sciences, Southwest University, Chongqing, 400716, China

* To whom correspondence should be addressed. E-mail: 030860005{at}webmail.hzau.edu.cn.


  Abstract

Genomic imprinting is theorized to exist in all placental mammals and some marsupials. Imprinted genes play important roles in the regulation of fetal growth, development and postnatal behavior, but the study of imprinted genes has been limited in livestock. In this study, the polymorphism-based approach was used to detect expression patterns of porcine pleiomorphic adenoma gene-like I (PLAGL1) and paternal expression gene 10 (PEG10) genes. Single nucleotide polymorphisms in exons were detected between the Meishan and Large White breeds in the PLAGL1 and PEG10 genes. The polymorphisms were used to determine the monoallelic or biallelic expression with reverse transcriptase-PCR-RFLP in 44 tissues from 4 heterozygous pigs (based on SNP). Imprinting analysis indicated that the PLAGL1 and PEG10 genes were both paternally expressed in all tested tissues (heart, liver, spleen, lung, kidney, stomach, small intestine, skeletal muscle, fat, uterus and ovary). Our study showed that the method of identifying polymorphic transcripts with reverse transcriptase-PCR-RFLP may be beneficial for detecting the imprinting status of some candidate imprinted genes.

Key Words: imprinting, paternal expression gene 10, pig, pleiomorphic adenoma gene-like I




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