J. Anim Sci.
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Published online first on May 25, 2007
J. Anim Sci. 1990. doi:10.2527/jas.2006-237
© 2007 American Society of Animal Science
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J. Anim Sci., doi: 10.2527/jas.2006-237
©Copyright, 2007, The American Society of Animal Science

ARTICLE

Changes in small intestinal nutrient transport and barrier function following lipopolysaccharide exposure in 2 pig breeds

D. M. Albin 1, J. E. Wubben 2, J. M. Rowlett 2, K. A. Tappenden 1, R. A. Nowak 2*

1 Division of Nutritional Sciences, University of Illinois, Urbana 61801
2 Department of Animal Sciences, University of Illinois, Urbana 61801

* To whom correspondence should be addressed. E-mail: ranowak{at}uiuc.edu.


  Abstract

Specific pig breeds with unique characteristics have been developed, and the present study sought to characterize some of these differences. Using modified Ussing chambers, electrophysiological mucosal transport of D-glucose, L-glutamine, L-proline, L-arginine, L-threonine, and glycylsarcosine was assessed in small intestinal tissues (duodenum, jejunum, ileum) taken from Yorkshire-based hybrid (BW = 142.4 ± 2.0 kg; mean age = 8 mo.) and Meishan (BW = 65.8 ± 0.8 kg; mean age = 6 mo) female pigs following 4 h lipopolysaccharide (LPS) exposure. Gilts were randomly assigned to control (saline infusion; n = 6 Yorkshires, n = 5 Meishans) or LPS (n = 7 Yorkshires, n = 5 Meishans) groups. Therefore, treatments were arranged in a 2 (breed) x 2 (LPS infusion) factorial. Four h after infusions, pigs were euthanized and intestinal segment samples were removed. Glucose transport in the ileum was decreased (P < 0.001) in Yorkshires with LPS, but was increased (P < 0.001) over 2-fold in Meishans. Following LPS infusion, proline transport was increased in duodenum (over 5-fold; P = 0.04) and ileum (over 10-fold; P < 0.001) of Meishans, while being unaffected in Yorkshires. Arginine transport in the ileum of control Meishans was greater (P = 0.05) than arginine transport in control Yorkshires. Glycylsarcosine transport was greater (P = 0.02) in Meishans than Yorkshires (nearly 2-fold), regardless of LPS provision. Glycylsarcosine transport was increased (P = 0.003) over 2-fold by LPS, regardless of pig breed. Resistance (barrier function) was increased (P = 0.03) by LPS in Yorkshires, but was unaffected in Meishans. The present study indicates that small intestinal function responded much differently to LPS in Yorkshire and Meishan gilts, and that these effects were nutrient and segment dependent.

Key Words: nutrient transport, barrier function, intestine, lipopolysaccharide, pig breed






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Copyright © 2007 by the American Society of Animal Science.