J. Anim Sci.
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J. Anim Sci. 2008. 86:1106-1113. doi:10.2527/jas.2007-0718
© 2008 American Society of Animal Science
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ANIMAL GROWTH, PHYSIOLOGY, AND REPRODUCTION

Trichostatin A and nuclear reprogramming of cloned rabbit embryos1

L. H. Shi*,{dagger}, J. S. Ai*,{dagger}, Y. C. OuYang*, J. C. Huang*,{dagger}, Z. L. Lei*,{dagger}, Q. Wang*,{dagger}, S. Yin*,{dagger}, Z. M. Han*, Q. Y. Sun* and D. Y. Chen*,2

* State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences; and {dagger} Graduate School, Chinese Academy of Sciences, Beijing, China

2 Corresponding author: chendy{at}ioz.ac.cn

To investigate the influence of histone deacetylases on nuclear reprogramming after nuclear transfer, we treated the cloned embryos with a histone deacetylase inhibitor, Trichostatin A (TSA). In the present study, global changes in acetylation of histone H3-lysine 14, histone H4-lysine 12, and histone H4-lysine 5 were studied in rabbit in vivo fertilized embryos, somatic cell nuclear transfer (SCNT) embryos, and TSA-treated SCNT embryos. From the pronuclear to the morula stage, the deacetylation-reacetylation changes in acetylation of histone H3-lysine 14 and histone H4-lysine 12 occurred in both fertilized embryos and TSA-treated cloned embryos; however, the distribution pattern in untreated cloned embryos failed to display such changes. More interesting, the signal of acetylation of histone H4-lysine 12 in cloned embryos was detected in both the inner cell mass and the trophectoderm, whereas TSA-treated cloned embryos showed the same staining pattern as fertilized embryos and the staining was limited to the inner cell mass. The histone acetylation pattern of TSA-treated SCNT embryos appeared to be more similar to that of normal embryos, indicating that TSA could improve nuclear reprogramming after nuclear transfer.

Key Words: histone acetylation • rabbit fertilized embryos • somatic cell nuclear transfer embryos • Trichostatin A






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