J. Anim Sci.
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J. Anim Sci. 2007. 85:571-576. doi:10.2527/jas.2006-366
© 2007 American Society of Animal Science
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ANIMAL GENETICS

Breed-associated variations in the sequence of the pig 3ß-hydroxysteroid dehydrogenase gene1

R.-A. Cue, S. I. Nicolau-Solano, J. D. McGivan, J. D. Wood and O. Doran2

Division of Farm Animal Science, School of Clinical Veterinary Science, University of Bristol, Langford, Bristol BS40 5DU, UK

2 Corresponding author: e.udovikova{at}bristol.ac.uk

The entire sequence of the pig 3ß-hy-droxysteroid dehydrogenase (3ß-HSD) gene has recently become known. This gene is deemed to be important in androstenone metabolism in pig liver, and its defective expression has been shown to be related to androstenone accumulation in adipose tissue and the development of boar taint. The aim of the present work was to do the following: 1) define the structure of the pig 3ß-HSD gene and 2) compare 3ß-HSD DNA sequences from pigs of different breeds, which vary in adipose tissue androstenone levels, with the purpose of identifying a polymorphism that might be responsible for differential 3ß-HSD expression. The 5'flanking and the coding region of 3ß-HSD were cloned and sequenced by conventional techniques. The 3ß-HSD coding regions were identical in pigs of different breeds and in animals with high and low androstenone levels. Significant sequence variations were found in the 5'flanking region of the 3ß-HSD gene, where differences in the number of TTAT repeats and 3 SNP were observed. The SNP were associated with the number of the TTAT repeats. These variations in the DNA sequence of the 3ß-HSD gene were not associated with the androstenone level in s.c. adipose tissue but were breed-dependent. The results of this work might be used for detection of the presence of Meishan genes in Western pig breeds, especially if the phenotype is not clearly established.

Key Words: breed • 3ß-hydroxysteroid dehydrogenase • gene • pig • polymorphism




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G. Chen, E. Bourneuf, S. Marklund, G. Zamaratskaia, A. Madej, and K. Lundstrom
Gene expression of 3{beta}-hydroxysteroid dehydrogenase and 17{beta}-hydroxysteroid dehydrogenase in relation to androstenone, testosterone, and estrone sulphate in gonadally intact male and castrated pigs
J Anim Sci, October 1, 2007; 85(10): 2457 - 2463.
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