J. Anim Sci.
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J. Anim. Sci. 2004. 82:319-328
© 2004 American Society of Animal Science

ANALYTICAL METHOD CHALLENGES FOR MEASURING NUTRIENTS AND ANTINUTRIENTS IN PLANTS SYMPOSIUM

Challenges and opportunities in the analysis of raffinose oligosaccharides, pentosans, phytate, and glucosinolates

D. V. Vinjamoori*,1, J. R. Byrum{dagger}, T. Hayes{ddagger} and P. K. Das*

* Crop Analytics, Monsanto Company, St. Louis, MO 63167; and {dagger} Applied Molecular Breeding Center, Monsanto Company, Ankeny, IA 50021; and and {ddagger} Calgene Campus, Monsanto Company, Davis, CA 95616

1 Correspondence: 800 N. Lindbergh Blvd., Mail Zone S4A (phone: 314-694-3674; e-mail: dvvinj{at}monsanto.com).

Abstract

In this paper, the status of the analytical technologies for assaying animal antinutritional compounds, such as raffinose oligosaccharides, pentosans, phytic acid, and glucosinolates, is reviewed in terms of selectivity, sensitivity, and sample throughput. The implementation of simplified sample preparation schemes, use of novel separation approaches, and alternate detector technologies are discussed. The challenges and opportunities posed by these assays are highlighted along with the recommendations for best analytical practices.

Key Words: Glucosinolates • Pentosans • Phytate • Raffinose • Stachyose






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