J. Anim Sci.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Huff-Lonergan, E.
Right arrow Articles by Robson, R. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Huff-Lonergan, E.
Right arrow Articles by Robson, R. M.

Journal of Animal Science, Vol 74, Issue 5 993-1008, Copyright © 1996 by American Society of Animal Science

JOURNAL ARTICLE

Proteolysis of specific muscle structural proteins by mu-calpain at low pH and temperature is similar to degradation in postmortem bovine muscle

E. Huff-Lonergan, T. Mitsuhashi, D. D. Beekman, F. C. Parrish Jr, D. G. Olson and R. M. Robson
Department of Animal Science, Iowa State University, Ames 50011, USA.

Postmortem (PM) and mu-calpain-induced degradation of specific skeletal muscle proteins was monitored by SDS-PAGE and Western blotting. Samples were removed from bovine longissimus thoracis (LT) at approximately 45 min PM for the preparation of at-death (0-d) myofibrils (MF). The LT was excised at 1 d PM, vacuum-packaged, and stored at 2 degrees C. Samples were removed for Warner-Bratzler shear force analysis and biochemical analysis at 1, 3, 7, 14, 28, and 56 d PM. The protease mu-calpain was purified from bovine skeletal muscle and used to digest at-death MF at pH 5.6, 4 degrees C, 100 microM CaCl2. Degradation of the proteins titin, nebulin, filamin, desmin, and troponin-T was monitored in the PM and mu-calpain-digested samples by using SDS-PAGE and Western blotting. The PM samples that had significantly lower shear force (LSF) values (P < .05) at 1 d PM exhibited faster degradation of these five proteins than the higher shear force (HSF) samples. In LSF samples, the intact titin band (T1) was absent by 7 d PM and nebulin was absent by 3 d PM. In LSF samples, some filamin was degraded by 3 d PM, but in HSF samples degradation was not apparent until 14 d PM. In LSF samples, desmin was degraded more rapidly PM than in HSF samples. Troponin-T was broken down PM to yield two major polypeptides of approximately 28 and 30 kDa; these polypeptides appeared earlier PM in LSF samples. Degradation products, similar to those observed PM, for all five proteins also were detected in Western blots of mu-calpain-digested MF, suggesting the calpain system plays a key role in PM protein degradation.


This article has been cited by other articles:


Home page
 J ANIM SCIHome page
J. Berard, M. Kreuzer, and G. Bee
Effect of litter size and birth weight on growth, carcass and pork quality, and their relationship to postmortem proteolysis
J Anim Sci, September 1, 2008; 86(9): 2357 - 2368.
[Abstract] [Full Text] [PDF]

Home page
 J ANIM SCIHome page
A. D. Weaver, B. C. Bowker, and D. E. Gerrard
Sarcomere length influences postmortem proteolysis of excised bovine semitendinosus muscle
J Anim Sci, August 1, 2008; 86(8): 1925 - 1932.
[Abstract] [Full Text] [PDF]

Home page
 J ANIM SCIHome page
K. M. Carnagey, E. J. Huff-Lonergan, A. Trenkle, A. E. Wertz-Lutz, R. L. Horst, and D. C. Beitz
Use of 25-hydroxyvitamin D3 and vitamin E to improve tenderness of beef from the longissimus dorsi of heifers
J Anim Sci, July 1, 2008; 86(7): 1649 - 1657.
[Abstract] [Full Text] [PDF]

Home page
 J ANIM SCIHome page
K. M. Carnagey, E. J. Huff-Lonergan, S. M. Lonergan, A. Trenkle, R. L. Horst, and D. C. Beitz
Use of 25-hydroxyvitamin D3 and dietary calcium to improve tenderness of beef from the round of beef cows
J Anim Sci, July 1, 2008; 86(7): 1637 - 1648.
[Abstract] [Full Text] [PDF]

Home page
 J ANIM SCIHome page
A. E. Hamling, B. E. Jenschke, and C. R. Calkins
Effects of aging on beef chuck and loin muscles enhanced with ammonium hydroxide and salt
J Anim Sci, May 1, 2008; 86(5): 1200 - 1204.
[Abstract] [Full Text] [PDF]

Home page
 J ANIM SCIHome page
J. P. Camou, J. A. Marchello, V. F. Thompson, S. W. Mares, and D. E. Goll
Effect of postmortem storage on activity of {micro}- and m-calpain in five bovine muscles
J Anim Sci, October 1, 2007; 85(10): 2670 - 2681.
[Abstract] [Full Text] [PDF]

Home page
 J ANIM SCIHome page
G. H. Geesink, S. Kuchay, A. H. Chishti, and M. Koohmaraie
{micro}-Calpain is essential for postmortem proteolysis of muscle proteins
J Anim Sci, October 1, 2006; 84(10): 2834 - 2840.
[Abstract] [Full Text] [PDF]

Home page
 J ANIM SCIHome page
K. R. M. Carlin, E. Huff-Lonergan, L. J. Rowe, and S. M. Lonergan
Effect of oxidation, pH, and ionic strength on calpastatin inhibition of {micro}- and m-calpain
J Anim Sci, April 1, 2006; 84(4): 925 - 937.
[Abstract] [Full Text] [PDF]

Home page
 J ANIM SCIHome page
G. Bee, C. Biolley, G. Guex, W. Herzog, S. M. Lonergan, and E. Huff-Lonergan
Effects of available dietary carbohydrate and preslaughter treatment on glycolytic potential, protein degradation, and quality traits of pig muscles
J Anim Sci, January 1, 2006; 84(1): 191 - 203.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
S. J. Franco and A. Huttenlocher
Regulating cell migration: calpains make the cut
J. Cell Sci., September 1, 2005; 118(17): 3829 - 3838.
[Abstract] [Full Text] [PDF]

Home page
 J ANIM SCIHome page
L. Kristensen, M. Therkildsen, M. D. Aaslyng, N. Oksbjerg, and P. Ertbjerg
Compensatory growth improves meat tenderness in gilts but not in barrows
J Anim Sci, December 1, 2004; 82(12): 3617 - 3624.
[Abstract] [Full Text] [PDF]

Home page
 J ANIM SCIHome page
L. J. Rowe, K. R. Maddock, S. M. Lonergan, and E. Huff-Lonergan
Oxidative environments decrease tenderization of beef steaks through inactivation of {micro}-calpain
J Anim Sci, November 1, 2004; 82(11): 3254 - 3266.
[Abstract] [Full Text] [PDF]

Home page
 J ANIM SCIHome page
J. L. Montgomery, M. B. King, J. G. Gentry, A. R. Barham, B. L. Barham, G. G. Hilton, J. R. Blanton Jr., R. L. Horst, M. L. Galyean, K. J. Morrow Jr., et al.
Supplemental vitamin D3 concentration and biological type of steers. II. Tenderness, quality, and residues of beef
J Anim Sci, July 1, 2004; 82(7): 2092 - 2104.
[Abstract] [Full Text] [PDF]

Home page
 J ANIM SCIHome page
A. E. Wertz, T. J. Knight, A. Trenkle, R. Sonon, R. L. Horst, E. J. Huff-Lonergan, and D. C. Beitz
Feeding 25-hydroxyvitamin D3 to improve beef tenderness
J Anim Sci, May 1, 2004; 82(5): 1410 - 1418.
[Abstract] [Full Text] [PDF]

Home page
 J ANIM SCIHome page
E. Veiseth, S. D. Shackelford, T. L. Wheeler, and M. Koohmaraie
Indicators of tenderization are detectable by 12 h postmortem in ovine longissimus
J Anim Sci, May 1, 2004; 82(5): 1428 - 1436.
[Abstract] [Full Text] [PDF]

Home page
 J ANIM SCIHome page
J. L. Melody, S. M. Lonergan, L. J. Rowe, T. W. Huiatt, M. S. Mayes, and E. Huff-Lonergan
Early postmortem biochemical factors influence tenderness and water-holding capacity of three porcine muscles
J Anim Sci, April 1, 2004; 82(4): 1195 - 1205.
[Abstract] [Full Text] [PDF]

Home page
 J ANIM SCIHome page
P. S. Kuber, J. R. Busboom, E. Huff-Lonergan, S. K. Duckett, P. S. Mir, Z. Mir, R. J. McCormick, M. V. Dodson, C. T. Gaskins, J. D. Cronrath, et al.
Effects of biological type and dietary fat treatment on factors associated with tenderness: I. Measurements on beef longissimus muscle
J Anim Sci, March 1, 2004; 82(3): 770 - 778.
[Abstract] [Full Text] [PDF]

Home page
 J ANIM SCIHome page
L. J. Rowe, K. R. Maddock, S. M. Lonergan, and E. Huff-Lonergan
Influence of early postmortem protein oxidation on beef quality
J Anim Sci, March 1, 2004; 82(3): 785 - 793.
[Abstract] [Full Text] [PDF]

Home page
 J ANIM SCIHome page
M. R. Foote, R. L. Horst, E. J. Huff-Lonergan, A. H. Trenkle, F. C. Parrish Jr., and D. C. Beitz
The use of vitamin D3 and its metabolites to improve beef tenderness
J Anim Sci, January 1, 2004; 82(1): 242 - 249.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
S. B. Marston and C. S. Redwood
Modulation of Thin Filament Activation by Breakdown or Isoform Switching of Thin Filament Proteins: Physiological and Pathological Implications
Circ. Res., December 12, 2003; 93(12): 1170 - 1178.
[Abstract] [Full Text] [PDF]

Home page
 J ANIM SCIHome page
D. A. King, M. E. Dikeman, T. L. Wheeler, C. L. Kastner, and M. Koohmaraie
Chilling and cooking rate effects on some myofibrillar determinants of tenderness of beef
J Anim Sci, June 1, 2003; 81(6): 1473 - 1481.
[Abstract] [Full Text] [PDF]

Home page
 J ANIM SCIHome page
S. Muroya, I. Nakajima, and K. Chikuni
Amino acid sequences of multiple fast and slow troponin T isoforms expressed in adult bovine skeletal muscles
J Anim Sci, May 1, 2003; 81(5): 1185 - 1192.
[Abstract] [Full Text] [PDF]

Home page
 J ANIM SCIHome page
L. Kristensen, M. Therkildsen, B. Riis, M. T. Sorensen, N. Oksbjerg, P. P. Purslow, and P. Ertbjerg
Dietary-induced changes of muscle growth rate in pigs: Effects on in vivo and postmortem muscle proteolysis and meat quality
J Anim Sci, November 1, 2002; 80(11): 2862 - 2871.
[Abstract] [Full Text] [PDF]

Home page
 J ANIM SCIHome page
B. R. Wiegand, J. C. Sparks, D. C. Beitz, F. C. Parrish Jr., R. L. Horst, A. H. Trenkle, and R. C. Ewan
Short-term feeding of vitamin D3 improves color but does not change tenderness of pork-loin chops
J Anim Sci, August 1, 2002; 80(8): 2116 - 2121.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1996 by the American Society of Animal Science.