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ANIMAL NUTRITION |



* Departments of Animal Science,
and
Human Nutritional Sciences, and
and
Food Science, University of Manitoba, Winnipeg, Manitoba, Canada R3T 2N2
| Abstract |
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Key Words: carcass quality corn growth performance pig
| INTRODUCTION |
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Because of the increase in corn production, the amount of locally grown corn that is available for use in making swine feed has increased. However, pigs fed corn-based diets could produce inferior quality products for some markets compared with pigs fed barley- or wheat-based diets in terms of meat and fat color because yellow corn contains higher amounts of carotenoids, naturally occurring fat soluble pigments often found in plants (NRC, 1998
; Prache et al., 2003
; Carr et al., 2005
).
Another product quality concern related to feeding corn-based diets to pigs is the production of soft fats due to the high content of unsaturated fatty acids (UFA) in corn. A high amount of UFA in pork has been associated with increased difficulties in belly slicing and such quality problem as susceptibility to rapid oxidative rancidity (Averette Gatlin et al., 2002
). According to Carr et al. (2005)
, pigs fed a corn-based diet had higher G:F than those fed a barley-based diet but similar carcass characteristics. However, Robertson et al. (1999)
reported that pigs fed corn-based diets had darker LM compared with those fed diets based on hulless barley. Thus, the effects of feeding corn vs. barley to swine on growth performance and carcass characteristics have not been conclusively determined.
Therefore, the objective of this study was to evaluate the effects of representative Manitoba-grown corn hybrids on growth performance and carcass characteristics of growing-finishing pigs using a barley-based diet as control.
| MATERIALS AND METHODS |
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The use of pigs and the experimental procedures were reviewed and approved by the Animal Care Committee of the University of Manitoba, and animals were cared for according to the standard guidelines of the Canadian Council on Animal Care (CCAC, 1993
).
Twenty-four Cotswold pigs (12 barrows and 12 gilts) with an average initial BW of 41.4 (SD = 1.4) kg were obtained from the University of Manitobas Glenlea Swine Research Farm for use in this study. Pigs were blocked on the basis of BW and sex into 8 blocks (4 blocks each of barrows and gilts) each with 3 pigs. Pigs from each block were randomly allotted to 1 of 3 treatment groups, resulting in a total of 8 pigs per treatment. The 3 dietary treatments consisted of a barley-based diet (control) and 2 diets each based on 1 of the 2 most cultivated corn hybrids in Manitoba, 39M27 (Pioneer Hi-Bred Ltd, Chatham, ON; corn 1) and 39W54 (Pioneer Hi-Bred Ltd, Chatham, ON; corn 2).
The barley sample is a 6-row barley hybrid and the corn hybrids are early maturing hybrids requiring less than 2,400 CHU, an equivalent of less than 75 d, to reach maturity (Dwyer et al., 1991
). The CHU rating of corn 1 and corn 2 were 2,150 and 2,100, respectively. Corn 1 is a genetically modified corn that is resistant to European corn borer. It contains a protein (Cry 3Bbl) from a bacterium, Bacillus thuringiensis, that is toxic to European corn borer and thus could potentially minimize the use of insecticides (MAFRI, 2004
; Hyun et al., 2005
). All grain samples were from the 2003 planting season harvest. The 2 corn samples were obtained from Carman, Manitoba, whereas the barley sample was obtained from the University of Manitoba Glenlea Research Station. The 2 locations were within 100 km of Winnipeg (49°53' N, 97°10' W), Canada.
The pigs were fed on a 3-phase dietary program for BW 20 to 50 kg (phase I), 50 to 80 kg (phase II), and 80 to 110 kg (phase III). Diets for each phase were formulated to meet the NRC (1998)
nutrient standards for mixed sexes, with a high to medium lean growth rate (Table 1
). The analyzed lysine (0.26 and 0.24% for corn 1 and corn 2, respectively) and other AA contents of the corn samples were similar to NRC (1998)
values (Opapeju, 2005
). Hence, the diets were formulated using NRC (1998)
values based on the total lysine content of the ingredients, and the calculated dietary levels of CP, total lysine, and DE were similar across dietary treatments within each phase.
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Carcass Evaluation
On reaching an average live BW of 107.8 (SD = 2.9) kg, pigs were slaughtered after 18 h of feed deprivation. Before slaughter, pigs were weighed and then given an intramuscular injection of acepromazine maleate (0.3 mg/kg of BW; Atravet 10 mg injectable, Wyeth Animal Health, Ontario, Canada) mixed in a single syringe with 15 mg of ketamine hydrochloride/kg of BW (Ketalean, Bimeda-MTC Animal Health Inc., Ontario, Canada) as a preanesthetic tranquilizer. Once tranquilized sufficiently, approximately 20 mg of sodium pentobarbital/ kg of BW (Bimeda-MTC Animal Health Inc.) was administered intravenously through the lateral ear vein to provide for a plane of surgical anesthesia but not enough to cause respiratory arrest. Pigs were then exsanguinated, and the viscera were removed. The dressing percent was determined as the ratio of HCW (head-on) to live BW at slaughter. The carcasses (head-on) were then split in 2 by cutting through the backbone and stored in a cooler at 4°C.
After 24 h in the cooler, carcass length was measured on the right half of the carcass as the distance between the cranial face of the first rib and the tip of the aitch bone. The belly was fabricated from the right half of the carcass according to the UN/ECE standard procedure for porcine carcasses and cuts (ECE, 1997
). Approximately 36 x 48 cm of the belly was removed and subjected to the belly flex test described by Rentfrow et al. (2003)
. Briefly, the carcass was centered on a polyvinyl chloride pipe (9-cm diam.) mounted perpendicularly on a board marked with a 2.54-cm grid matrix with the skin side down and the chine side against the board. Lateral and vertical flexes were calculated as the average of the lateral and vertical left and right flexes determined relative to the grid matrix on the left and right side of the board, respectively. About 50 g of leaf fat was removed for belly fat fatty acid analysis.
The left half of the carcass was evaluated for backfat thickness, loin depth, and LM area (LMA). Midline backfat thickness was measured perpendicular to the skin at the first and last rib and at the last lumbar vertebra. The carcass was cut using the Hobart meat-cutting machine (The Hobart Mfg. Co., Ltd., Toronto, Canada) between the 10th and 11th ribs to determine the 10th rib fat thickness, LM depth, and LMA. The outline of the LM was traced on acetate paper, and the LMA was later determined using a 0.25-cm2 grid. Samples of subcutaneous fat were obtained from each pig at the 10th and 11th ribs for backfat fatty acid and fat color analysis, respectively. The samples, about 50 g for fatty acid analysis and approximately 8 x 10 cm for fat color analysis, were carefully separated from the attached muscle. All fat samples were rinsed with saline solution (0.9%) and stored at 80°C until further analysis.
Fat color was evaluated according to the Commission International de lEclairage (CIE) L* (lightness), a* (red-green scale), and b* (yellow-blue scale) values (CIE, 1976
) using a Minolta Spectrophotometer CM-3500d (Minolta Co., Ltd., Osaka, Japan). The spectrophotometer was calibrated using the manufacturers zero calibration and white, standard, calibration caps. The L* scale ranged from 0 to 100%; that is, the greater the value, the lighter the color; the a* scale ranged from negative (green) to positive (red); and the b* scale ranged from negative (blue) to positive (yellow). Each sample was read thrice.
Fatty Acid Analysis
Feed ingredient samples (corn and barley) and belly fat and backfat samples were analyzed for fatty acids. A representative sample of each grain ground through a 1-mm screen and thoroughly mixed was subjected to lipid extraction according to AOAC (1990)
procedures. Extracted oil was methylated following the procedure of Folch et al. (1957)
. Belly fat and backfat samples were extracted and methylated according to the procedures of Folch et al. (1957)
.
Fatty acid methyl esters were analyzed using a Hewlett-Packard 5890A gas chromatograph equipped with an autosampling injection system HP 7673 (Hewlett-Packard Inc., Avondale, AZ). The 100-m-long column, an Agilent HP-88 capillary column (J and W Scientific, Folsom, CA), was made of fused silica coated with 88% cyanopropyl aryl siloxane (0.25-mm i.d. and 0.2-µm film thickness). The column temperature began at 50°C, and after 1 min, it increased at a rate of 20°C/min to 160°C and was held at this level for 23 min. The temperature then increased at a rate of 2°C/min to 210°C and was held there for 7 min. To clean the column between samples, the column temperature was increased at a rate of 20°C/min to 240°C and was held at this level for 5 min. The flame ionization detector temperature was kept constant at 300°C. The fatty acid methyl esters were identified using a methyl ester standard GLC-461 (Nu Check Prep Inc., Elysian, MN).
Statistical Analysis
All data were subjected to ANOVA with a randomized complete block design using the GLM procedures of SAS (SAS Inst. Inc., Cary, NC). The effects of sex (df = 1), block within sex (df = 6), and dietary treatment (df = 2) were included in the model as sources of variation. The individual pig was considered as the experimental unit. When a significant F-value (P < 0.05) was indicated by the ANOVA, dietary treatment means were separated and compared using Tukeys test.
| RESULTS AND DISCUSSION |
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Growth Performance and Carcass Characteristics
The effects of dietary treatment on ADG, ADFI, and G:F are presented in Table 4
. There were no differences in ADG, ADFI, and G:F of pigs fed the barley-based diets compared with those fed diets based on either of the 2 corn hybrids during any phase of the study or overall. Similarly, Carr et al. (2005)
did not detect any differences in ADG, ADFI, and G:F between pigs (45 to 80 kg of BW) fed a barley-based diet and those fed a corn-based diet.
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There was no effect of dietary treatment on the L*, a*, and b* fat color (Table 5
). Similar to the result of the current study, Carr et al. (2005)
did not find any differences in L*, a*, and b* fat color of pigs fed the diet based on barley compared with those fed the corn-based diet. Corn has higher amount of pigments compared with barley (NRC, 1998
), and this could contribute to the deposition of color fat (Carr et al. 2005
). Although we did not measure carotenoid concentration of the grain samples used in the current study, differences in the concentration of pigments between corn and barley, if there were any, did not affect pork fat color.
Fatty Acid Profiles of the Backfat and Belly Fat
The effects of dietary treatment on the profile of fatty acid in backfat are presented in Table 6
. The backfat of pigs on the barley-based diet tended to have higher concentration of pentadecanoic acid (15:0; P = 0.092), palmitoleic acid (16:1; P = 0.080), and heptadecanoic acid (17:0; P = 0.089) compared with those fed the diet based on corn 2. Pigs on the barley-based diet had a higher (P < 0.05) concentration of heptadecenoic acid (17:1; P = 0.006) in their backfat compared with those that received diets based on corn. The concentration of eicosenoic acid (20:1) in the backfat of pigs fed diets based on barley and corn 1 was higher (P < 0.05) compared with those fed diet based on corn 2. The backfat of pigs fed the diet based on corn 2 had higher (P < 0.05) concentrations of linoleic acid (18:2) and PUFA compared with pigs fed the diet based on barley. Linoleic acid (18:2) is the only PUFA that was significantly (P = 0.038) affected by dietary treatment, and it is prob ably the component responsible for the dietary differences observed in the concentration of PUFA in the backfat of the pigs. Others have reported a positive correlation between linoleic acid (18:2) and PUFA in pork fat (Averette Gatlin et al., 2002
; Rentfrow et al., 2003
). Despite the fact that the backfat from pigs fed the diet based on corn 2 had a higher concentration of PUFA compared with those fed the diet based on barley, the concentration of PUFA in the backfat of all pigs regardless of dietary treatment was within the range (less than 23%) recommended for salami making (Warnants et al., 1998
). The fatty acid profiles of backfat of pigs fed the barley- and corn-based diets in the current study are similar to the values reported by Carr et al. (2005)
for these feedstuffs. The differences in the fatty acid composition of barley and the 2 corn hybrids used in the current study did not affect the deposition of SFA and UFA in the backfat of pigs. The concentration of SFA and UFA in the backfat was not different across dietary treatments.
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There was a treatment x sex interaction (P < 0.05) on lauric acid (12:0) and oleic acid (18:1) contents of the belly fat. Although there was no difference in the lauric acid concentration in the belly fat of gilts and barrows receiving the diets based on barley and corn 1, the barrows fed the diet based on corn 2 had a higher (P = 0.018) value compared with the gilts (0.11 vs. 0.09%). Similarly, the gilts fed the diet based on corn 2 had a higher (P = 0.035) concentration of oleic acid (36.23 vs. 31.29%) in their belly fat compared with the barrows, but the concentration was not different for the gilts and barrows on the barley and corn 1 diets.
Recently developed hybrids of corn grown in Manitoba are specifically bred for lower CHU, hence the need to know how low CHU influence the nutrient profile of corn and eventually growth performance and carcass characteristics. It is obvious in this study there were no differences in growth performance and carcass quality of growing-finishing pigs fed diets based on the low-CHU-rated hybrids of corn that are available in Manitoba and those fed the barley-based diet. Likewise, there was no difference in the feeding value of corn 1 (B. thuringiensis corn) and that of corn 2. Assuming a relatively similar feed intake in ad libitum-fed pigs in a commercial setting, body fatty acid composition should not be a concern when these corn hybrids are used instead of barley in swine diets as long as the diets are formulated to meet lysine:energy requirements of the growing-finishing pigs.
| Footnotes |
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2 Corresponding author: martin_nyachoti{at}umanitoba.ca
Received for publication July 3, 2005. Accepted for publication June 1, 2006.
| LITERATURE CITED |
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