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J. Anim. Sci. 2005. 83:430-439
© 2005 American Society of Animal Science

ANIMAL PRODUCTS

Effect of endophyte type on carcass traits, meat quality, and fatty acid composition of beef cattle grazing tall fescue

C. E. Realini*, S. K. Duckett*,1, N. S. Hill{dagger}, C. S. Hoveland{dagger}, B. G. Lyon{ddagger}, J. R. Sackmann* and M. H. Gillis*

* Animal and Dairy Science Department and and {dagger} Crop and Soil Sciences Department, University of Georgia, Athens 30602; and and {ddagger} ARS, USDA, Richard B. Russell Research Center, Athens, GA 30604


   Abstract
Top
 Abstract
Introduction
 Materials and Methods
 Results and Discussion
 Implications
 Literature Cited
 
Fourteen Hereford steers were used to compare carcass traits, meat quality, and fatty acid composition of beef from cattle grazing tall fescue infected with either wild-type (E+; n = 6) or novel, nil ergot alkaloid (AR542; n = 8) endophyte for 209 d. Average daily gain, live weight, and HCW were greater (P < 0.05) for AR542 cattle than for E+. No differences in LM color or pH were observed between AR542 and E+. Steaks from E+ cattle tended (P = 0.10) to have higher L* and b* than those from AR542 cattle at 0 d of display. Ground beef from E+ cattle also had higher (P < 0.05) L* than AR542 cattle, with no differences in a* or b* at 0 d of display. Color changes during display did not differ for both steaks and ground beef from E+ and AR542. Lipid oxidation levels increased (P < 0.05) during simulated retail display, but they did not differ between endophyte treatments. Adipose tissues from E+ cattle had a higher (P < 0.05) percentage of SFA, and a lower (P < 0.05) percentage of MUFA than adipose from AR542 cattle. Ground beef and i.m. fat had higher (P < 0.05) concentrations of SFA, MUFA, and cis-9, trans-11 isomer of conjugated linoleic acid, and lower (P < 0.05) concentrations of PUFA and PUFA:SFA ratio than s.c. fat. The n-6:n-3 fatty acid ratio did not differ among fat depots. Ergot-alkaloids were detected in s.c. adipose tissues, and alkaloid concentration was greater (P < 0.05) for E+ than AR542. Warner-Bratzler shear force values did not differ between endophyte types, but it decreased (P < 0.01) across the postmortem aging period. Conversely, sensory panel evaluation detected greater (P < 0.01) chewiness and lower (P < 0.05) juiciness for AR542 than for E+ steaks aged for 14 d. Although grazing cattle on tall fescue pastures infected with nil ergot alkaloid endophyte improved cattle performance, these results suggest that endophyte type has minor effects on carcass traits and meat quality of pasture-fed beef. Moreover, finishing cattle on tall fescue pastures showed the potential to enhance the fatty acid profile of beef from a human health perspective. Alkaloid concentration was greater (P < 0.05) in s.c. fat from E+ than AR542 (2.81 vs. 0.92 ppb; fresh-tissue basis). This is the first published report demonstrating the presence of alkaloids in beef tissues.

Key Words: Beef Quality • Ergot Alkaloids • Tall Fescue


   Introduction
Top
 Abstract
 Introduction
Materials and Methods
 Results and Discussion
 Implications
 Literature Cited
 
Tall fescue (Festuca arundinacea), a major component of pasture systems in the United States (over 14 million ha), is frequently infected with the endophyte fungus Neotyphodium coenophialum. The fungal endophyte shares a symbiotic relationship with tall fescue, protecting the host plant against disease, insects, and drought. At the same time, it has been identified as the causative agent of fescue toxicosis. Some of the symptoms associated with toxicosis include increased respiration rates, rectal temperatures, salivation, nervousness, and rough hair coats, and decreased weight gains and overall performance in beef steers (Hoveland et al., 1983Go; Stuedemann and Hoveland, 1988Go). Endophyte-infected tall fescue contains various alkaloids that are candidate toxins presumed to cause the toxico-sis syndrome. Stuedemann et al. (1998)Go and Hill et al. (2001)Go proposed that the polar ergot alkaloids (lysergamides, lysergic acid, and lysergol) are the toxic entities causing fescue toxicosis.

Management and grazing recommendations have been suggested for alleviating tall fescue toxicosis in beef cattle. More recently, endophytes that produce nil ergot alkaloids have been incorporated into tall fescue cultivars (Bouton et al., 2002Go) and are commercially available in the United States (Max-Q, Pennington Seed, Madison, GA). Current research has shown that tall fescue pastures infected with nil ergot alkaloid endophyte are a promising alternative for combating toxicosis and greatly improve beef productivity (Bouton et al., 2002Go; Parish et al., 2003Go; S. K. Duckett, unpublished data). However, no data are available on how endophyte type alters carcass characteristics and beef quality in cattle finished on tall fescue pastures or whether endophytes are deposited in beef tissues. Therefore, the objectives of this study were to determine the effect of endophyte type (novel, nil ergot alkaloid vs. wild-type) in beef cattle grazing tall fescue on carcass traits, meat quality, and fatty acid composition, as well as to detect concentrations of ergot alkaloids, if possible, in s.c. fat.


   Materials and Methods
Top
 Abstract
 Introduction
 Materials and Methods
Results and Discussion
 Implications
 Literature Cited
 
Fourteen Hereford steers (273 ± 35 kg) grazed replicated 0.8 ha/paddock tall fescue pastures infected with either wild-type (E+; two pasture replicates; three steers per pasture) or novel, nil ergot alkaloid (AR542; four pasture replicates; two steers per pasture) endophyte for 74 d from October to December 2000, and for 102 d from March to July 2001 at the Central Georgia Experiment Station, Eatonton. Put-and-take grazing management was used throughout the grazing season to maintain similar forage availability among treatments. All steers used in this study grazed their respective paddocks for the entire 176-d grazing period. Steers were provided with water, minerals, and shade in each paddock. During the winter months (December to March), steers were maintained on a common pasture and supplemented with bermudagrass hay. These cattle were part of a larger grazing experiment conducted over a 3-yr period (1999 to 2001), and additional grazing information has been reported by Parish et al. (2003)Go. Animal handling procedures for this study were approved by the University of Georgia Animal Care and Use Committee.

At the end of the grazing period, steers were slaughtered at a commercial meat plant, and carcass data collected included adjusted fat thickness, LM area, marbling score, percentage of KPH fat, skeletal maturity, and USDA yield and quality grades. Carcasses were fabricated according to National Association of Meat Purveyors (NAMP) specifications (NAMP, 1988Go), and the ribeye roll (NAMP 112) and chuck roll (NAMP 114) were removed from each carcass, vacuum-packaged, and transported to the University of Georgia Meat Science Laboratory. Upon arrival, pH was measured in three locations of the LM at the 12th rib using a spear-tip electrode (model 8163BN, Orion, Beverly, MA) connected to a portable pH meter with automatic temperature compensation (model 59002-30, Cole-Parmer, Vernon Hills, IL). The ribeye roll was further processed into steaks for fatty acid analysis, tenderness, sensory panel evaluation, and lipid and color stability measurements. Steaks designated for fatty acid analysis, shear force measurements, and sensory panel evaluation were individually vacuum packaged and frozen at –20°C for subsequent analysis. The clod was ground (0.635 cm) and prepared into 114-g patties. Steaks and patties for lipid and color stability measurements were individually placed on Styrofoam trays, wrapped with oxygen-permeable film, and displayed at 4°C in a cooler illuminated with 1,614-lx fluorescent lighting. Lipid oxidation and objective color measurements were taken on steaks and patties at 0, 5, 12, and 21 d, and 0, 2, 4, and 8 d of display, respectively.

Instrumental Color

Instrumental color measurements were recorded for L* (measures darkness to lightness; lower L* indicates a darker color), a* (measures redness; higher a* value indicates a redder color), and b* (measures yellowness; higher b* value indicates a more yellow color) using a Minolta chromameter (CR-310, Minolta Inc., Osaka, Japan) with a 50-mm-diameter measurement area using a D65 illuminant, which was calibrated using the ceramic disk provided by the manufacturer. Color readings were determined at 24 h postmortem on s.c. fat and the exposed LM after ribbing between the 12th and 13th ribs. Beef color measurements of LM steaks and ground beef were obtained at 0, 5, 12, and 21 d, and 0, 2, 4, and 8 d of display, respectively. Values were recorded from three locations of the upper surface of each steak and ground beef sample randomly selected to obtain a representative reading of the surface color.

Lipid Oxidation Analysis

Lipid stability was evaluated in the same steaks and ground beef that were displayed for instrumental color. Lipid oxidation was determined by measuring 2-thiobarbituric acid reactive substances (Jo and Ahn, 1998Go) at 0, 5, 12, and 21 d of display for steaks, and at 0, 2, 4, and 8 d of display for ground beef.

Fatty Acid Composition

Longissimus steaks, ground beef, and s.c. fat samples were submerged in liquid N (–196°C), pulverized, and stored anaerobically at –20°C. Total lipid was determined following the chloroform-methanol procedure of Folch et al. (1957)Go, modified by using a 10:1 ratio of chloroform-methanol to sample. Extract containing approximately 25 mg of lipid was converted to fatty acid methyl esters following the method of Duckett et al. (2002)Go. The fatty acid methyl esters were analyzed by GC (Agilent 6890; Aligent Technologies, Wilmington, DE), and separated using a 100-m capillary column (0.25-mm i.d. and 0.20-µm film thickness, SP 2560; Supelco, Bellefonte, PA). Column oven temperature was programmed at 150 to 165°C at 1°C/min, 165 to 167°C at 0.2°C/min, 167 to 225°C at 1.5°C/min, and held at 225°C for 15 min with 1:100 split. Injector and detector temperatures were maintained at 250°C. Hydrogen was the carrier gas at a flow rate of 1 mL/min. Individual fatty acids were identified by comparison of retention times with standards (obtained from Sigma Chemical, St. Louis, MO; Supelco and Matreya, Pleasant Gap, PA).

Ergot Alkaloids in Fat

Ergot alkaloids were extracted from 5 g of homogenized fat by adding 1 mL of 0.1 M NaOH with 20 mL chloroform. The homogenate was vortexed for 5 min and filtered through a Whatman No. 41 filter paper. Five milliliters of 0.1 M hydrochloric acid was added to the chloroform with 15 mL of water, inverted to mix the solutions, and 15 mL of the aqueous layer were pipetted from the phase-separated sample. The pH of the decanted aqueous solutions was adjusted to 6.5 with 0.2 M NaOH, the samples frozen, and lyophilized. The lyophilized samples (salt plus alkaloids) were extracted with methanol, and taken to dryness in a vacuum chamber. Extracted samples were then analyzed for ergot alkaloids via a competitive ELISA as described by Adcock et al. (1997)Go, using the ergot alkaloid-specific monoclonal antibody (15F3.E5).

Warner-Bratzler Shear Force

Steaks (2.5 cm thick) were vacuum-packaged, stored in a cooler at 4°C, and frozen after 2, 4, 8, 14, and 21 d of aging for subsequent Warner-Bratzler shear force determination. Steaks were thawed for 24 h at 4°C, and broiled on Farberware (Bronx, NY) electric grills to an internal temperature of 71°C (AMSA, 1995Go). Steaks were allowed to cool to room temperature before six 1.27-cm-diameter cores were removed from each steak parallel to the longitudinal orientation of the muscle fibers. All cores were sheared perpendicular to the long axis of the core using a TA-XT2 texture analyzer (Texture Technologies Corp., Scarsdale, NY) equipped with a Warner-Bratzler knife, and peak shear force was recorded. Crosshead speed was set at 20 cm/min.

Sensory Panel Evaluation

Steaks (2.5 cm thick) were vacuum-packaged, stored in a cooler at 4°C, and frozen at –20°C after 14 d of aging for subsequent trained sensory analyses. Steaks were thawed for 24 h at 4°C, and broiled on Farberware electric grills to an internal temperature of 71°C (AMSA, 1995Go). Steaks were immediately cut into 2.54 cm x 1.27 cm x 1.27 cm cubes, and served warm to a nine-member sensory panel trained according to AMSA (1995)Go guidelines. Each panelist evaluated two cubes from each sample for juiciness (amount of juice perceived in the mouth) and chewiness (amount of work it takes to get the samples ready to swallow) using a five-point scale (1 = not at all juicy or chewy, 5 = extremely juicy or chewy).

Statistical Analyses

Data were analyzed as a completely randomized design using the GLM procedure of SAS (SAS Inst., Inc., Cary, NC) with animal as the experimental unit. Lipid oxidation, objective color, and Warner-Bratzler shear force data were analyzed using repeated measures analysis, with endophyte type tested by the between-subjects error, and time and interaction between time and endophyte type tested by the within-subjects error term. Least squares means were generated and separated using the PDIFF option of SAS for main or interactive effects. Fatty acid data were analyzed as a 2 x 3 factorial with endophyte type (E+ and AR542), adipose tissue location (ground beef, LM, and s.c. fat), and two-way interaction in the model. There were no interactions between endophyte type and adipose tissue location for lipid content and fatty acid composition of the samples, with the exception of myristoleic acid. Thus, the fatty acid data are reported as main effects for endophyte type and adipose tissue location. Simple correlations between growth rate, fat depth, HCW, and LM color with Warner-Bratzler shear force values were computed using the correlation procedure of SAS. Significance was determined at P ≤ 0.05, whereas differences of P > 0.05 to P ≤ 0.10 were considered as trends.


   Results and Discussion
Top
 Abstract
 Introduction
 Materials and Methods
 Results and Discussion
Implications
 Literature Cited
 
Animal Performance and Carcass Traits

Effect of endophyte type on animal performance and carcass characteristics is presented in Table 1Go. Average daily gain was greater (P < 0.05) by 0.22, 0.49, and 0.37 kg/d for AR542 than E+ for fall, spring, and total grazing season, respectively. Live weight and HCW were greater (P < 0.05) for steers grazing AR542 than the steers grazing E+ infected tall fescue. Previous research has shown that tall fescue pastures infected with AR542 were promising alternatives for combating toxicosis and improving cattle growth rates and beef productivity (Parish et al., 2003Go; S. K. Duckett, unpublished data). Carcass traits, including quality and yield grades, did not differ (P ≥ 0.15) between the endophyte types.


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  		Table 1. Least squares means (±SE) for steer performance and carcass characteristics as affected by endophyte type
 
Instrumental Color, pH, and Lipid Oxidation

Effects of endophyte type on muscle pH, LM color, and s.c. fat color are shown in Table 2Go. Longissimus muscle pH at 24 h postmortem did not differ (P = 0.87) between treatments, and no differences (P > 0.17) in color (L*, a*, and b*) were observed for LM or s.c. fat between AR542 and E+ cattle. Longissimus muscle L* values reported here for pasture-finished beef were lower (darker) than those reported for concentrate-finished beef (Bidner et al., 1986Go; Bennett et al., 1995Go; McCaughey and Cliplef, 1996). Pasture-finished cattle typically have a more yellow fat color due to greater concentration of ß-carotene than carcasses from concentrate-fed cattle (Bennett et al., 1995Go; Simonne et al., 1996Go).


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  		Table 2. Least squares means (±SE) for longissimus muscle pH and color, and subcutaneous fat color as affected by endophyte type
 
Steaks from E+ cattle tended to have higher (P = 0.10) L* and b* values than those from AR542 cattle on d 0 of simulated retail display (Table 3Go). There were no differences (P = 0.22) in steak redness (a*) associated with fescue endophyte type. Ground beef from E+ cattle was lighter (higher L* value; P < 0.05) than ground beef from AR542 cattle; however, a* and b* values did not differ (P > 0.18) between endophyte treatments. There were no differences (P > 0.22) in steak or ground beef color between the treatments measured at 5, 12, and 21 d, and 2, 4, and 8 d of display, respectively (results not shown). Lipid oxidation did not differ in ground beef (P = 0.36) or LM (P = 0.62) samples between endophyte treatments, and the interaction between endophyte type and time was non-significant (P = 0.16; results not shown). Lipid oxidation increased (P < 0.01) during simulated retail display.


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  		Table 3. Least squares means (±SE) for longissimus muscle steak and ground beef color on d 0 of simulated display as affected by endophyte type
 
Lipid Content and Fatty Acid Composition

The effect of endophyte type on lipid content and fatty acid composition pooled over adipose tissue location (ground beef, i.m., and s.c. fat) is presented in Table 4Go. No interactions (P > 0.17) were detected between endophyte type and adipose tissue location for lipid content and fatty acid composition, with the exception of myristoleic (C14:1) acid (P < 0.05). There were no differences between E+ and AR542 in the myristoleic acid content of ground beef and s.c. fat (0.42 vs. 0.44% and 0.30 vs. 0.38%, respectively), whereas C14:1 was higher (P < 0.05) in the i.m. fat from AR542 than E+ (0.87 vs. 0.47%, respectively).


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  		Table 4. Least squares means (±SE) for fatty acid composition (g/100 g of total fatty acids) pooled over three fat depots (ground beef, longissimus muscle, and subcutaneous fat) as affected by endophyte type
 
Total lipid content was unaffected (P = 0.45) by endophyte type. Endophyte type did not alter (P > 0.14) the proportions of myristic (C14:0), palmitic (C16:0), linoleic (C18:2), linolenic (C18:3), arachidonic (C20:4), eicosapentaenoic (C20:5), docosapentaenoic (C22:5), or docosahexaenoic (C22:6) acids, unidentified fatty acids, or the percentage of total PUFA.

Adipose tissues from E+ cattle had higher (P < 0.05) proportions of stearic (C18:0) acid, and lower (P < 0.05) proportions of palmitoleic (C16:1) and oleic (C18:1) acids than AR542. Consequently, the percentage of SFA was higher (P < 0.05), and the percentage of MUFA was lower (P < 0.05) in the adipose tissues of E+ steers compared with AR542 steers.

Numerous disorders occur in livestock grazing infected tall fescue, and several clinical signs of toxicosis are consistent with the development of bovine fat necrosis. These include increased body temperature, decreased performance, and rough hair coat (Stuedemann et al., 1975Go; Hoveland et al., 1983Go; Stuedemann and Hoveland, 1988Go). Steers grazing E+ endophyte-infected tall fescue in this study exhibited these clinical signs of toxicosis. Stuedemann and Hoveland (1988)Go proposed that toxic tall fescue influences lipid metabolism, and that there may be a link between poor animal performance on tall fescue and occurrence of fat necrosis. Increased body temperature caused by heat stress or administration of a pyrogenic substance has been shown to alter lipid metabolism, resulting in lower blood cholesterol concentrations (Noble et al., 1973Go; O’Kelly and Reich, 1975Go). Stuedemann et al. (1985)Go reported that necrotic fat contains more CP and ash, with less ether-extractable material, and three to four times higher cholesterol content than normal fat. Rumsey et al. (1979)Go studied the chemical composition of necrotic fat lesions in beef cows grazing fertilized "Kentucky-31" tall fescue, and reported that the molar proportion of stearic acid was greater, whereas the proportions of oleic and palmitoleic acids were lower, in the necrotic fat residue than in normal fat residue. Steers slaughtered in this study did not show any occurrence of nercrotic fat tissue in the perirenal region. Necrotic fat lesions have typically only been reported in aged cows with extended exposure to E+ tall fescue. Although we did not observe the appearance of necrotic fat lesions, results from the fatty acid composition analysis (higher C18:0 and lower C14:1, C16:1, and C18:1 in E+ fat) suggest that fescue toxicosis may influence lipid metabolism and contribute to the occurrence of fat necrosis. The greater concentration of stearic acid combined with the lower percentage of MUFA, particularly oleic acid, in adipose tissues from E+ cattle suggests that fescue toxicosis may inhibit {Delta}-9 desaturase, which is responsible for the conversion of stearic acid to oleic acid in adipose tissues.

Adipose tissue from AR542 cattle tended (P = 0.08) to have a higher proportion of CLA isomer cis-9, trans-11 and total CLA than E+. The n-6:n-3 ratio did not differ (P > 0.72) between E+ and AR542 cattle, and these levels are considered beneficial from a human health perspective compared to higher ratios reported in the literature for feedlot cattle (4.15, French et al., 2000Go; 6.38, Rule et al., 2002Go).

Total lipid content and fatty acid composition of ground beef, LM, and s.c. fat from the rib pooled over endophyte type (E+ vs. AR542) are shown in Table 5Go. As expected, total fatty acid content of the three tissues differed (P < 0.01) greatly, with s.c. fat containing the highest amount (56.7%) and LM containing the lowest (1.46%). Fatty acid profile shows that the lipid composition of i.m. fat more closely resembled that of ground beef than s.c. fat. The proportions of myristic, palmitic, stearic, and oleic acids did not differ between ground beef and LM, but were higher (P < 0.05) for these tissues than in s.c. fat. Consequently, the percentage of total SFA was lower (P < 0.05) in s.c. fat compared with either ground beef or LM. The concentrations of palmitoleic acid and total MUFA were lower (P < 0.05) in s.c. than LM and ground beef, which did not differ.


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  		Table 5. Least squares means for fatty acid composition (g/100 g of total fatty acids) of ground beef, longissimus muscle (i.m.) fat, and subcutaneous fat (SQ) pooled across endophyte types
 
The proportions of the PUFA, including linoleic, linolenic, eicosapentaenoic, docosapentaenoic, and docosahexaenoic acids, were highest (P < 0.05) in s.c. fat and lowest (P < 0.05) in ground beef. Arachidonic acid concentrations were higher (P < 0.05) in s.c. than LM and ground beef fat. As a result, the proportion of total PUFA and the PUFA:SFA ratio were higher (P < 0.05) for s.c. fat than the LM, with ground beef fat being intermediate. In contrast, LM typically contains higher levels of PUFA than s.c. fat in beef from concentrate-finished cattle (Gillis et al., 2004Go). Mitchell et al. (1991)Go indicated that lipid content and fatty acid composition varied with tissue site reporting higher concentrations of PUFA and lower concentrations of MUFA in i.m. fat compared to s.c. fat. These differences in PUFA between forage-finished and concentrate-finished beef are likely the result of greater dietary linolenic acid intake throughout the finishing period and lower rates of fat accretion for forage-fin-ished compared with concentrate-finished cattle.

Differences between adipose tissues were not (P = 0.18) observed for the n-6:n-3 fatty acid ratio. Concentrations of total CLA and CLA isomer cis-9, trans-11 were lower (P < 0.05) in subcutaneous fat compared to either LM or ground beef. French et al. (2000)Go reported similar CLA concentrations in LM for grass-fed beef (10.8 mg of CLA/g of lipid). Previous research has shown that including grass in the diet of dairy and beef cattle increased CLA concentration in milk and intramuscular fat, respectively (Lawless et al., 1998Go; French et al., 2000Go; Yang et al., 2002Go). Shantha et al. (1997)Go reported 7.7 and 5.2 mg total CLA/g lipid in semimembranosus muscle for grass-fed and corn supplemented, grass-fed beef, respectively. Rule et al. (2002)Go reported 4.1 and 2.6 mg CLA cis-9, trans-11/g lipid in LM for pasture-fed cows and feedlot steers, respectively. The CLA concentrations reported in this study were higher than values reported for LM from feedlot cattle (cis-9, trans-11 = 0.599%, and total CLA = 0.919%; Gillis et al., 2004Go). French et al. (2000)Go reported 3.7 mg total CLA/g lipid for beef cattle fed concentrates, whereas Mir et al. (2000)Go reported 1.7 mg of total CLA/g of lipid for cattle fed a barley-based diet.

The CLA concentrations reported for ground beef in this study were higher than previously published values. Chin et al. (1992)Go reported CLA concentrations in ground beef of 4.3 mg/g of lipid, whereas Shantha et al. (1994)Go reported a range in CLA concentrations for ground beef from the chuck between 6.6 and 8.2 mg/g of fat. Both Chin et al. (1992)Go and Shantha et al. (1994)Go obtained samples from retail market; thus, the different nutritional background of the steers in this study would likely explain the higher levels observed.

Ergot Alkaloids

Subcutaneous fat from E+ cattle had higher (P < 0.01) concentrations of ergot alkaloids than AR542 (Figure 1Go). These results show that ergot alkaloids ingested during grazing were deposited in adipose tissue. Presence of ergot alkaloids detected in s.c. fat from AR542 cattle may be a result of exposure of steers to endophyte-infected tall fescue with toxic endophyte before this experiment. This is, to the authors’ knowledge, the first published report showing that ergot alkaloids accumulate in adipose tissues when cattle graze tall fescue pastures infected with toxic endophyte.



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  		Figure 1. Effect of endophyte type (E+ = wild type endophyte-infected tall fescue, or AR542 = novel, nil ergot alkaloid endophyte-infected tall fescue) on the content of ergot-alkaloids in subcutaneous fat (P = 0.01) from E+ and AR542 cattle (fresh-tissue basis).

 
Previous research has shown that approximately 94% of the ergot alkaloids are excreted in the urine of cattle grazing endophyte-infected tall fescue and that urinary alkaloids are rapidly metabolized and voided from urine when steers are removed from pastures (Stuedemann et al., 1998Go; Hill et al., 2000Go). Rapid excretion and clearance of ergot alkaloids in urine suggests that cattle finished on concentrates after grazing endophyte-infected tall fescue would not exhibit carryover effects from fescue toxicosis during the finishing phase. However, toxicosis symptoms have been reported for feedlot cattle that previously grazed endophyte-infected tall fescue compared to endophyte free or nonergot alkaloid-producing endophyte-infected pastures (S. K. Duckett, unpublished data). Ergot alkaloids present in fat tissues from cattle previously grazing endophyte-infected tall fescue may be metabolized during the feedlot phase, causing residual toxicosis symptoms and poor cattle performance.

Warner-Bratzler Shear Force and Sensory Analysis

Shear force values did not differ (P = 0.16) between endophyte types (Figure 2AGo), but decreased (P = 0.01) across the postmortem aging curve (Figure 2BGo). Initial shear force values (9 kg) reported here would be classified as "tough" and would be predicted to not reach threshold levels (4.6 kg) for acceptable tenderness according to Shackelford et al. (1997)Go. Shear force values decreased 2.5 and 4.3 kg from initial values to d 4 and d 14 postmortem, respectively. At 14 d of aging, shear force values decreased to 4.6 kg, a level that would be on the upper limit of threshold WBSF values considered acceptable for tenderness by consumers (3.0 to 4.6 kg at d 14 for 93% consumer tenderness acceptability, Miller et al., 2001Go; <4.6 kg at d 14, Shackelford et al., 1997Go). Changes in shear force reported here for pasture-finished beef suggest that the postmortem aging response reported for pasture-finished beef may differ from those typically reported for concentrate-finished beef. Previous research comparing tenderness of pasture- and concentrate-finished beef has produced mixed results on palatability attributes. Some studies found a negative effect of forage-finishing on meat tenderness (Smith, 1990Go; Mitchell et al., 1991Go), whereas others showed that grass-fed beef can be produced with no deleterious effects on meat quality, including tenderness (Mandell et al., 1998Go; French et al., 2001Go). In many experiments, dietary effects were confounded with other factors known to influence tenderness (animal age, growth rate, carcass weight, and external fat cover), and multiple postmortem aging times have not been evaluated. Additional research is needed to assess differences in postmortem myofibrillar degradation and calpain proteolytic system during postmortem aging between pasture- and concentrate-finished beef.



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  		Figure 2. Effect of: A) endophyte type (E+ = wild type endophyte-infected tall fescue, or AR542 = novel, nil ergot alkaloid endophyte-infected tall fescue; P = 0.16); and B) postmortem aging (P = 0.01) on Warner-Bratzler shear force (WBSF) values.

 
Steaks from AR542 steers were rated higher (P = 0.01) in chewiness and were less (P = 0.08) juicy than steaks from E+ steers aged for 14 d (Table 6Go). Cattle that grow more rapidly before slaughter have increased rates of protein turnover, resulting in higher concentrations of proteolytic enzymes in carcass tissues at slaughter which, in turn, may affect collagen solubility and/or myofibril fragmentation (Aberle et al., 1981Go; Hall and Hunt, 1982Go; Miller et al., 1983Go). Numerous studies (Bowling et al., 1978Go; Lochner et al., 1980Go; Dolezal et al., 1982Go) have reported a positive correlation between muscle tenderness, carcass weight, and fatness in feedlot cattle. However, other research has shown no relationship between carcass weight or carcass fat score and WBSF (French et al., 2001Go). In the present study, there were no significant correlations between ADG, fat depth or WBSF, whereas HCW was positively correlated with WBSF (r = 0.68; P < 0.05) measured at 2 d postmortem.


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  		Table 6. Least squares means (±SE) for sensory panel ratings of longissimus muscle steaks as affected by endophyte type
 

   Implications
Top
 Abstract
 Introduction
 Materials and Methods
 Results and Discussion
 Implications
Literature Cited
 
Nil ergot alkaloid endophyte-infected tall fescue is a promising alternative method to combat fescue toxicosis, and it greatly improves steer growth rate, as well as finishing and carcass weights. Nonetheless, results from this study suggest that endophyte type has minor effects on carcass traits and meat quality of grass-fed beef. Fescue toxicosis seems to influence fatty acid metabolism and may be involved in fat necrosis. This is the first published report showing that ergot alkaloids ingested during grazing are deposited in adipose tissue, which may explain residual toxicosis symptoms observed in feedlot cattle. Finishing cattle on tall fescue pastures may enhance the fatty acid profile of beef, including conjugated linoleic acid and n-3 fatty acids.

1 Correspondence—phone: 706-542-0942; fax: 706-542-0399; e-mail: sduckett{at}uga.edu.

Received for publication January 7, 2004. Accepted for publication November 10, 2004.


   Literature Cited
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 Abstract
 Introduction
 Materials and Methods
 Results and Discussion
 Implications
 Literature Cited
 


Aberle, E. D., E. S. Reeves, M. D. Judge, R. E. Hunsley, and T. W. Perry. 1981. Palatability and muscle characteristics of cattle with controlled weight gain: Time on a high-energy diet. J. Anim. Sci. 52:757–763.[Abstract/Free Full Text]

Adcock, R. A., N. S. Hill, J. H. Bouton, H. R. Boerma, and G. O. Ware. 1997. Symbiont regulation and reducing ergot alkaloid concentration by breeding endophyte-infected tall fescue. J. Chem. Ecol. 23:691–704.

AMSA. 1995. Research Guidelines for Cookery, Sensory Evaluation and Instrumental Tenderness Measurements of Fresh Meat. Am. Meat Sci. Assoc. and Natl. Livestock and Meat Board, Chicago, IL.

Bennett, L. L., A. C. Hammond, M. J. Williams, W. E. Kunkle, D. D. Johnson, R. L. Preston, and M. F. Miller. 1995. Performance, carcass yield, and carcass quality characteristics of steers finished on rhizoma peanut (Arachis glabrata)-tropical grass pasture or concentrate. J. Anim. Sci. 73:1881–1887.[Abstract]

Bidner, T. D., N. R. Schupp, A. B. Mohamad, N. C. Rumore, R. E. Montgomery, C. P. Bagley, and K. W. McMillin. 1986. Acceptability of beef from Angus-Hereford or Angus-Hereford-Brahman steers finished on all forage or a high energy diet. J. Anim. Sci. 62:381–387.[Abstract/Free Full Text]

Bouton, J. H., G. C. M. Latch, N. S. Hill, C. S. Hoveland, M. A. McCann, R. H. Watson, J. A. Parish, L. L. Hawkins, and F. N. Thompson. 2002. Reinfection of tall fescue cultivars with non-ergot alkaloid-producing endophytes. Agron. J. 94:567–574.[Abstract/Free Full Text]

Bowling, R. A., J. K. Riggs, G. C. Smith, Z. L. Carpenter, R. L. Reddish, and O. D. Butler. 1978. Production, carcass and palatability characteristics of steers produced by different management systems. J. Anim. Sci. 46:333–341.[Abstract/Free Full Text]

Chin, S. F., W. Liu, J. M. Storkson, Y. L. Ha, and M. W. Pariza. 1992. Dietary sources of conjugated dienoic isomers of linoleic acid, a newly recognized class of anticarcinogens. J. Food Compos. Anal. 5:185–187.

Dolezal, H. G., G. C. Smith, J.W. Savell, and Z. L. Carpenter. 1982. Comparison of subcutaneous fat thickness, marbling and quality grade for predicting palatability of beef. J. Food Sci. 47:397–401.

Duckett, S. K., J. G. Andrae, and F. N. Owens. 2002. Effect of high-oil corn or added corn oil on ruminal biohydrogenation of fatty acids and conjugated linoleic acid formation in beef steers fed finishing diets. J. Anim. Sci. 80:3353–3360.[Abstract/Free Full Text]

Folch, J., M. Lees, and G. H. Sloane Stanley. 1957. A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226:497–509.[Free Full Text]

French, P., E. G. O’Riordan, F. J. Monahan, P. J. Caffrey, M. T. Mooney, D. J. Troy, and A. P. Moloney. 2001. The eating quality of meat of steers fed grass and/or concentrates. Meat Sci. 57:379–386.

French, P., C. Stanton, F. Lawless, E. G. O’Riordan, F. J. Monahan, P. J. Caffrey, and A. P. Moloney. 2000. Fatty acid composition, including conjugated linoleic acid, of intramuscular fat from steers offered grazed grass, grass silage, or concentrate-based diets. J. Anim. Sci. 78:2849–2855.[Abstract/Free Full Text]

Gillis, M. H., S. K. Duckett and J. R. Sackmann. 2004. Effects of supplemental rumen-protected conjugated linoleic acid or corn oil on fatty acid composition of adipose tissues in beef cattle. J. Anim. Sci. 82:1419–1427.[Abstract/Free Full Text]

Hall, J. B., and M. C. Hunt. 1982. Collagen solubility of A-activity bovine longissimus muscle as affected by nutritional regimen. J. Anim. Sci. 55:321–329.[Abstract/Free Full Text]

Hill, N. S., J. A. Stuedemann, F. N. Thompson, G. W. Rottinghaus, H. J. Ju, J. K. Porter, D. L. Dawe, and E. E. Hiatt. 2001. Ergot alkaloid transport across ruminant digestive tissues. J. Anim. Sci. 79:542–549.[Abstract/Free Full Text]

Hill, N. S., F. N. Thompson, J. A. Stuedemann, D. L. Dawe, and E. E. Hiatt, III. 2000. Urinary alkaloid excretion as a diagnostic tool for fescue toxicosis. J. Vet. Diagn. Invest. 12:210–217.[Abstract/Free Full Text]

Hoveland, C. S., S. P. Schmidt, C. C. King, Jr., J. W. Odom, E. M. Clark, J. A. McGuire, L. A. Smith, H. W. Grimes, and J. L. Holliman. 1983. Steer performance and association of Acremonium coenophialum fungal endophyte on tall fescue pasture. Agron. J. 75:821–824.[Abstract/Free Full Text]

Jo, C., and D. U. Ahn. 1998. Fluorometric Anaysis of 2-Thiobarbituric Acid and Reactive Substances in Turkey. Poult. Sci. 77:475–480.[Abstract/Free Full Text]

Lawless, F., J. J. Murphy, D. Harrington, R. Devery, and C. Stanton. 1998. Elevation of conjugated cis-9, trans-11-octadecadienoic acid in bovine milk because of dietary supplementation. J. Dairy Sci. 81:3259–3267.[Abstract]

Lochner, J. V., R. G. Kauffman, and B. B. Marsh. 1980. Early postmortem cooling rate and beef tenderness. Meat Sci. 4:227–241.

Mandell, I. B., J. G. Buchanan-Smith, and C. P. Campbell. 1998. Effects of forage vs grain feeding on carcass characteristics, fatty acid composition, and beef quality in Limousin-cross steers when time on feed in controlled. J. Anim. Sci. 76:2619–2630.[Abstract/Free Full Text]

McCaughey, W. P., and R. L. Clipef. 1996. Carcass and organoleptic characteristics of meat from steers grazed on alfalfa/grass pastures and finished on grain. Can. J. Anim. Sci. 76:149–152.

Miller, M. F., M. A. Carr, C. B. Ramsey, K. L. Crockett, and L. C. Hoover. 2001. Consumer thresholds for establishing the value of beef tenderness. J. Anim. Sci. 79:3062–3068.[Abstract/Free Full Text]

Miller, R. K., J. D. Tatum, H. R. Cross, R. A. Bowling, and R. P. Clayton. 1983. Effects of carcass maturity on collagen solubility and palatability of beef from grain finished steers. J. Food Sci. 48:484–486.

Mir, Z., L. J. Paterson, and P. S. Mir. 2000. Fatty acid composition and conjugated linoleic acid content of intramuscular fat in crossbred cattle with and without Wagyu genetics fed a barley-based diet. Can. J. Anim. Sci. 80:195–197.

Mitchell, G. E., A. W. Reed, and S. E. Rogers. 1991. Influence of feeding regimen on the sensory qualities and fatty acid contents of beef steaks. J. Food Sci. 56:1102–1106.

NAMP. 1988. The Meat Buyer’s Guide. N. Am. Meat Proc. Assoc., Reston, VA.

Noble, R. C., J. C. O’Kelly, and J. H. Moore. 1973. Observations on changes in lipid composition and lecithin-cholesterol-acyl transferase reaction of bovine plasma induced by heat exposure. Lipids 8:216–223.[Medline]

O’Kelly, J. C., and H. P. Reich. 1975. Plasma lipid changes in cattle during chronic hyperthermia induced by heat exposure and by pyrogen. Comp. Biochem. Physiol. 51B:283–288.

Parish, J. A., M. A. McCann, R. H. Watson, N. N. Paiva, C. S. Hoveland, A. H. Parks, B. L. Upchurch, N. S. Hill, and J. H. Bouton. 2003. Use of nonergot alkaloid-producing endophytes for alleviating tall fescue toxicosis in stocker cattle. J. Anim. Sci. 81:2856–2868.[Abstract/Free Full Text]

Rule, D. C., K. S. Broughton, S. M. Shellito, and G. Maiorano. 2002. Comparison of muscle fatty acid profiles and cholesterol concentrations of bison, beef cattle, elk, and chicken. J. Anim. Sci. 80:1202–1211.[Abstract/Free Full Text]

Rumsey, T. S., J. A. Stuedemann, S. R. Wilkinson, and D. J. Williams. 1979. Chemical composition of necrotic fat lesions in beef cows grazing fertilized "Kentucky-31" tall fescue. J. Anim. Sci. 48:673–682.

Shackelford, S. D., T. L. Wheeler, and M. Koohmaraie. 1997. Tenderness classification of beef: I. Evaluation of beef longissimus shear force at 1 or 2 days postmortem as a predictor of aged beef tenderness. J. Anim. Sci. 75:2417–2422.[Abstract/Free Full Text]

Shantha, N. C., A. D. Crum, and E. A. Decker. 1994. Evaluation of conjugated linoleic acid concentrations in cooked beef. J. Agric. Food Chem. 42:1757–1760.

Shantha, N. C., W. G. Moody, and Z. Tabeidi. 1997. A research note: conjugated linoleic acid concentration in semimembranosus muscle of grass- and grain-fed and zeranol-implanted beef cattle. J. Muscle Foods 8:105–110.

Simonne, A. H., N. R. Green, and D. I. Bransby. 1996. Consumer acceptability and b-carotene content of beef as related to cattle finishing diets. J. Food Sci. 61:1254–1280.

Smith, G. C. 1990. Pages 152–162 in Quality of beef from cattle fed solely on forage. Texas Agric. Exp. Stn., Texas A & M University, College Station.

Stuedemann, J. A., N. S. Hill, F. N. Thompson, R. A. Fayrer-Hosken, W. P. Hay, D. L. Dawe, and D. H. Seman. 1998. Urinary and biliary excretion of ergot alkaloids from steers grazing endophyte-infected tall fescue. J. Anim. Sci. 76:2146–2153.[Abstract/Free Full Text]

Stuedemann, J. A., and C. S. Hoveland. 1988. Fescue endophyte: History and impact on animal agriculture. J. Prod. Agric. 1:39–44.

Stuedemann, J. A., T. S. Rumsey, J. Bond, S. R. Wilkinson, L. P. Bush, D. J. Williams, and A. B. Caudle. 1985. Association of blood cholesterol with occurrence of fat necrosis in cows and tall fescue summer toxicosis in steers. Am. J. Vet. Res. 46:1990–1995.[Medline]

Stuedemann, J. A., S. R. Wilkinson, D. J. Williams, H. Ciordia, J. V. Ernst, W. A. Jackson, and J. B. Jones, Jr. 1975. Long-term broiler litter fertilization of tall fescue pastures and health and performance of beef cows. Pages 264–268 in Managing Livestock Wastes, Proc. 3rd Int. Symp. Livestock Wastes, Champaign, IL. ASAE, St. Joseph, MI.

Yang, A., M. C. Lanari, M. Brewster, and R. K. Tume. 2002. Lipid stability and meat color of beef from pasture- and grain-fed cattle with or without vitamin E supplement. Meat Sci. 60:41–50.


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