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J. Anim. Sci. 1990. 68:1049-1160
© 1990 American Society of Animal Science


COMPOSITION AND QUALITY OF BEEF FROM STEERS SIRED BY PIEDMONTESE, GELBVIEH AND RED ANGUS BULLS1

J. D. Tatum, K. W. Gronewald2, S. C. Seideman3 and W. D. Lamm

Colorado State University4, Fort Collins 80523 and U.S. Department of Agriculture, ARS, Clay Center, NE 68933


    Abstract
 Top
 Abstract
 Introduction
 Materials and Methods
 Results
 Implications
 Literature Cited
 
Forty-five steers produced by matings of Piedmontese (P), Gelbvieh (G) and Red Angus (RA) sires to British (B) and Continental crossbred (Cx) dams were started on a finishing diet at an average of 291 d. Five steers from each sire-breed group were slaughtered after 124, 166 or 208 d of finishing. Age-constant values for slaughter weight and estimated degree of maturity did not differ for the three sire breed groups. Carcasses produced by P steers had the least fat thickness, the largest longissimus muscle (LM) areas, the lowest numerical yield grades, the highest yields of separable muscle and the highest muscle-to-bone ratios. Additionally, LM samples from P-sired steers had the highest percentage of white muscle fibers, the lowest percentage of intermediate muscle fibers and the smallest cross-sectional area of red muscle fibers. Marbling scores and intramuscular lipid content were highest for RA steers; P and G steers had similar values for marbling and intramuscular lipid content. Longissimus steaks from G steers received the lowest ratings for tenderness, flavor intensity and amount of connective tissue. Steaks from P and RA steers received similar sensory panel ratings. No differences were observed among the three sire-breed groups for amount and solubility of intramuscular collagen or for partitioning of separable carcass fat.

Key Words: Beef Cattle • Breeds • Carcass Composition • Meat Quality • Muscle Fibers


    Introduction
 Top
 Abstract
 Introduction
 Materials and Methods
 Results
 Implications
 Literature Cited
 
The Piedmontese breed originated in north-west Italy (Piedmonte e Liguria) and has remained concentrated in a relatively small geographic area (Siccardi, 1973Go). Sartore and Chiappone (1982)Go reported that over 90% of the pedigreed cattle in the breed were located in the Italian provinces of Cuneo, Turin and Asti. Throughout their early history, Piedmontese cattle were used principally for draft purposes, but also for production of milk and meat (Siccardi, 1973Go). Today, however, the breed is characterized by a high frequency of animals exhibiting "double muscling," or "muscular hypertrophy," and is known for its superior yields of lean meat (Masoero and Poujardieu, 1982Go).

There is considerable variation in expression of double muscling within the Piedmontese breed; cattle generally are divided into three classes based on phenotype: normal, intermediate and hypertrophied. Masoero (1982)Go reported that the registered herd makeup was 1% normal, 42% intermediate and 57% hypertrophied for males and 12% normal, 68% intermediate and 20% hypertrophied for females. Bulls classified as hypertrophied are preferred for use as herd sires, whereas cows classified as intermediate are considered to be most productive and, therefore, are preferred by breeders (Sartore and Chiappone, 1982Go).

Piedmontese cattle recently were introduced into the U.S. Because of their superior muscularity and leanness, Piedmontese sires may be particularly well suited for use in crossbreeding systems designed to produce high-cutability market cattle. However, the performance of Piedmontese in U.S. production systems has not been documented. This study was conducted to compare carcass characteristics of F1 Piedmontese steers with those of contemporary steers sired by Gelbvieh and Red Angus bulls.


    Materials and Methods
 Top
 Abstract
 Introduction
 Materials and Methods
 Results
 Implications
 Literature Cited
 
Animals.

Forty-five male calves were produced by matings of four Piedmontese (P) classified as hypertrophied, five Gelbvieh (G) and four Red Angus (RA) sires to British and Continental crossbred dams. Cows classified as British (n = 26) were mostly Hereford x Black or Red Angus, although four straightbred Hereford cows also were included in this group. Continental crossbred cows (n = 19) were 25 to 50% Simmental (S) or Charolais (C) and 25 to 75% Hereford (H) or Red Angus. Sixteen of the Continental crossbred cows were three-way crosses (S x H x RA or C x H x RA). British dams produced 9 RA, 6 P and 11 G steers; continental dams produced 6 RA, 9 P and 4 G steers. The choice of sire breeds permitted comparison of P (a breed selected for double muscling) with G (a European dual-purpose breed) and RA (a British breed selected for conventional beef characteristics).

The calves were born February through May of 1984 at the Eastern Colorado Research Center near Akron, Colorado. At birth, the calves were individually identified and birth dates were recorded. At branding (May 15, 1984), all calves were vaccinated, branded with a hot iron, castrated and dehorned (if necessary). All calves nursed their dams under similar pasture conditions until weaning (October 1, 1984). After weaning, the steers were backgrounded on corn silage. On January 9, 1985, the steers, none of which would be classified as double-muscled, were started on a high-concentrate finishing diet and fed for 124, 166 or 208 d prior to slaughter. During the finishing period, the steers had ad libitum access to cracked corn and were fed 4.5 kg com silage (wet weight) and .45 kg protein supplement per head daily.

Carcass Data Collection and Sample Preparation.

On each of the three slaughter dates, five steers from each sire-breed group were weighed individually (unshrunk) and transported 145 km to a commercial packing facility. Immediately following slaughter, hot carcass weight was recorded and the carcasses were chilled at approximately 2°C. Following a 24-h chill, each carcass was ribbed between the 12th and 13th ribs and factors used to determine USDA quality grade (carcass maturity and marbling) and yield grade (subcutaneous fat thickness, ribeye area and estimated kidney, pelvic and heart fat percentage) were obtained by a panel of three experienced carcass evaluators (USDA, 1989Go). Additionally the panel assigned scores to each carcass for color, texture and firmness of lean based on visual and physical characteristics of the surface of the M. longissimus at the 12th-13th rib interface. The following day, one side of each carcass was transported to the Colorado State University meat laboratory.

Within 96 h postmortem, each carcass side was fabricated into primal and subprimal cuts and each cut was dissected into muscle, fat and bone (bone plus cartilage and major tendons) using procedures described by Abraham et al. (1980)Go. Individual weights were recorded for each cut and its dissected components. Component weights corresponding to a particular tissue type (muscle, bone or fat) were aggregated to provide the weights of muscle, bone and fat in the entire side. Additionally, weights for individual fat components from each side were aggregated to provide the weights of the following fat depots: 1) subcutaneous fat -external fat covering the peripheral musculature, including scrotal fat and fat beneath the M cutaneous truncii and M. cutaneous omobrachialis; 2) intermuscular fat - fat between the muscles and between the musculature and bones or tendons; and 3) internal fat – fat within the body cavity, including the kidney, pelvic and heart depots together with fat removed from the internal surfaces of the ribs. Carcass side weight was expressed as the aggregate of all component weights.

Immediately following carcass dissection, a portion of the M. longissimus was removed from each short loin immediately posterior to the 12th rib. These samples were trimmed of all subcutaneous fat, vacuum-packaged individually, placed in an insulated shipping container and transported to the U.S. Meat Animal Research Center at Clay Center, Nebraska for proximate analysis, muscle fiber typing and intramuscular collagen assays. In addition, the 6th to 8th rib section of the M. longissimus from each carcass was vacuum-packaged, aged at 5°C until the 11th d postmortem and stored at –20°C to be used for taste panel testing.

Proximate Analysis and Collagen Assays.

Triplicate tissue samples (2 g) of the 12th rib section of the longissimus muscle were weighed, dried at 100°C for 12 h and reweighed to determine moisture content. The dried samples then were extracted for 48 h using diethyl either in a soxhlet extraction apparatus. The extracted samples were redried and weighed to determine lipid content (AOAC, 1980Go).

Freeze-powdered longissimus samples (4 g) were heated for 70 min at 77°C in .25-strength Ringer’s solution (Hill, 1966Go). Supernatant fluid and residue fractions were hydrolyzed in 6 N HCl for 20 h at 115°C. Following neutralization, hydroxyproline content of each hydrozylate was determined using spectrophotometric methods described by Bergman and Loxley (1963)Go. Collagen content was calculated by multiplying the hydroxyproline content of the residue by 7.25 and that of the supernatant fluid by 7.52 (Cross et al., 1973Go). Percentage of soluble (heat labile) collagen was calculated by dividing the collagen content of the supernatant fluid by the collagen content of the entire sample.

Palatability Determinations.

Sensory evaluation was performed by a 10-member descriptive panel. Panelists were selected and trained in accordance with the AMSA Guidelines for Cooking and Sensory Evaluation of Meat (AMSA, 1978Go).

Three steaks (2.54 cm thick) were removed from the frozen 6th to 8th rib section of the longissimus from each carcass. The steaks were thawed at 2°C and broiled on Farberware Open-Hearth broilers to an internal temperature of 70°C (monitored using copper-constantan thermocouples and a recording potentiometer). The 8th rib steak was used for sensory evaluation; the 6th and 7th rib steaks were used for subsequent shear force measurements.

Upon reaching the desired internal temperature, steaks for sensory evaluation were removed from the broiler and portioned into sections of uniform dimensions (approximately 1.3 cm x 1.3 cm x 1.9 cm). The warm sections were selected randomly and served immediately to the panel. Panelists assigned scores to each sample for juiciness, myofibrillar tenderness, connective tissue amount and flavor intensity using 8-point, structured rating scales.

Steaks for shear force determinations were cooled to 20°C and a minimum of six 1.27-cm cores were removed parallel to the longitudinal orientation of the muscle fibers for Warner-Bratzler shear force measurements.

Muscle Fiber Type Determinations.

Histological samples were removed from the medial, central and lateral sections of the 13th rib section of the longissimus muscle. The samples were frozen in liquid nitrogen, wrapped in aluminum foil and stored at –63°C. Transverse sections (10 µm thick) of each muscle sample were cut using a cryostat and stained for alkali-stable ATPase using procedures described by Guth and Samaha (1970)Go. Serial sections were stained for succinate dehydrogenase activity according to procedures outlined by Troyer (1980)Go. Photomicrographs were obtained for each sample and individual muscle fibers were counted and classified as red, intermediate or white based on staining intensity. The mean area of 10 fibers of each type was determined using a Bioquant particle size analyzer. Data from the three muscle locations were averaged prior to statistical analysis.

Statistical Methods.

The data were analyzed using a least squares model that included the fixed effects of sire breed and dam breed-type. Additionally, linear regressions of traits on initial age (age at the onset of the finishing period) and days on feed were included in the model and variation due to the interaction between the days on feed regression and sire breed was partitioned. Subclass days on feed regressions were used to adjust the means for an additional comparison of the breed groups at a common degree of marbling using procedures outlined by Koch et al. (1979)Go. When F-tests for sire breed were significant, subclass means were compared using Tukey’s w procedure (Steel and Torrie; 1960Go).


    Results
 Top
 Abstract
 Introduction
 Materials and Methods
 Results
 Implications
 Literature Cited
 
Slaughter Traits.

Mean squares for slaughter weight and estimated degree of maturity at slaughter are presented in Table 1Go. Age-constant least squares means for these traits, corresponding to the effects of sire breed, are provided in Table 2Go.


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TABLE 1. MEAN SQUARES FOR SLAUGHTER, YIELD GRADE AND QUALITY GRADE TRAITS
 

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TABLE 2. AGE-CONSTANT LEAST SQUARES MEANS SHOWING THE EFFECTS OF SIRE BREED ON SLAUGHTER TRAITS, CARCASS GRADE TRAITS, CARCASS COMPOSITION AND FAT PARTITIONING
 
Taylor (1985)Go advocated scaling growth and carcass data by mature weight in order to permit comparisons of biologically diverse genotypes at comparable stages of developmental maturity. In the present study, sire breed did not influence (P > .05) age-constant values for slaughter weight or estimated degree of maturity at slaughter. Correspondingly, age-constant comparisons of sire breeds also reflect differences among P-, G- and RA-sired steers at comparable slaughter weights and degrees of maturity.

Breed type of dam was a significant (P < .05) source of variation in slaughter weight, but it did not affect (P > .05) estimated degree of maturity at slaughter (Table 1Go). Steers produced by Continental crossbred dams were heavier than those produced by British dams (528.5 kg vs 502.2 kg). Slaughter weight and estimated degree of maturity increased (P > .05) with increased time on feed (Table 1Go).

Dressing Percentage.

Least squares means for dressing percentage (Table 2Go) showed that P-sired steers had 1.2 and 1.6% higher dressed yields than G- and RA-sired steers, respectively. However, due to the limited number of animals used in this study, these differences in dressing percentage were not of sufficient magnitude for statistical significance (P = .15). Dressing percentage was not significantly affected by dam breed type or days on feed (Table 1Go).

In a review of double muscling and its effects on meat production traits, Boccard (1981)Go concluded that dressing percentages for homozygous double-muscled animals normally are about 5% higher than those for normal animals of the same breed, sex and weight. Boccard (1981)Go attributed this advantage in dressing percentage to hypotrophy of several major internal organs, including the spleen, liver and digestive tract. The weights of these organs have been shown to be from 14 to 37% lower for double-muscled than for normal animals (Boccard, 1981Go). Thiessen and Rollins (1982)Go compared British crossbred calves that were heterozygous for double muscling with normal calves from similar matings. In their study, heterozygous bulls and heifers had 1.7% higher dressing percentages than did normal bulls and heifers.

Information concerning dressed yields of Piedmontese F1 cattle is limited. Siccardi (1973)Go reported results from three small crossbreeding studies in which Piedmontese sires were mated to Dutch Friesian, Brown Swiss and Valdostana P.R. dams. Progeny (male and female) from these matings were compared to purebred progeny (male and female) of the three dam breeds. The comparisons of F1 Piedmontese with Dutch Friesian (DF) and Valdostana P.R. (V) involved calves slaughtered at very light weights (F1 = 174.3 kg vs DF = 163.7 kg and F1 = 206.7 kg vs V = 184.3 kg). Compared to DF and V calves, the F1 calves had 4.3 and 4.2 higher dressing percentages, respectively. In the third comparison, F1 Piedmontese (slaughtered at 492 kg) had 9.9% higher dressed yields than did purebred Brown Swiss (slaughtered at 434 kg).

Bonsembiante et al. (1975)Go compared dressed yields of crossbred bulls (500 kg) produced by mating Red Pied, Piedmontese, Romagnola, Chianina and Marchigiana bulls to Brown Alpine and Friesian cows. In their study, F1 Piedmontese bulls had from 1.4 to 2.1% higher (P < .01) dressing percentages than did F1 bulls from the other four sire breeds. These differences in dressed yield are similar in magnitude to the mean differences observed between steers sired by P bulls and steers sired by G or RA bulls in our study.

Carcass Grade Traits.

Mean squares for carcass yield grade traits are presented in Table 1Go. Breed of sire was a significant source of variation in adjusted fat thickness, longissimus area and yield grade but had no effect on hot carcass weight or estimated percentage of kidney, pelvic and heart fat. Fat thickness was highest (P < .05) for RA-, intermediate for G-and lowest (P < .05) for P-sired steers (Table 2Go). Sire breed groups ranked in the reverse order (P > G > RA) for longissimus area. Differences in yield grade (RA > G > P) reflected the effects of sire breed on fat thickness and longissimus area (e.g., the P-sired steers were leanest and had the largest longissimus areas and therefore had the lowest numerical yield grades). Effects of dam breed on carcass yield grade traits were not significant (Table 1Go). The significant effect of days on feed for fat thickness, longissimus area, hot carcass weight and kidney, pelvic and heart fat percentage (Table 1Go) reflected an increase (P < .05) in each trait with increased time on feed.

Results for carcass quality grade traits are presented in Tables 1Go and 2Go. Significant effects of sire breed, dam breed type, days on feed and the interaction between sire breed and days on feed were observed for marbling score (Table 1Go). On an age-constant basis, steers sired by RA bulls had higher (P < .05) marbling scores than G- and P-sired steers (Table 2Go); marbling scores for the latter two groups (G and P) were not different (P > .05). Steers out of B dams had higher (P < .05) marbling scores than those produced by Cx dams (British = 432.5, Cx = 398.4).

Marbling score increased as time on feed increased. Moreover, tests of significance for differences among subclass regression coefficients revealed that RA-sired steers responded to increased feeding time (days) with greater (P < .05) increases in marbling score (numerical value) than either of the other two sire-breed groups (bRA = 1.32, bP = .80, bG = .71). Because of this difference in rate of marbling deposition, RA-sired steers required fewer days on feed to deposit a "small" degree of marbling (the minimum amount of marbling required for A maturity carcasses to grade Choice). In fact, RA-sired steers already had attained a "small" degree of marbling by the first slaughter date (124 d on feed). Steers sired by P and G bulls required approximately 170 and 190 d on feed, respectively, to attain a "small" degree of marbling. Carcass maturity increased (P < .01) as days on feed increased but was unaffected by either sire breed or dam breed type (Table 1Go).

West et al. (1973)Go compared carcass grade traits of heterozygous double-muscled steers with those of normal steers. In their study, the two genotypes did not differ in marbling score or USDA quality grade; however, heterozygous double-muscled steers had significantly less fat thickness, larger longissimus areas and higher estimated cutability (lower yield grades) than normal steers. Thiessen and Rollins (1982)Go reported similar differences in yield grade traits among carcasses produced by heterozygous double-muscled and normal bulls and heifers, except that in their study differences in fat thickness were not significant. Carroll et al. (1978)Go compared carcass quality grade traits of heterozygous double-muscled heifers and bulls with those of normal heifers and bulls. Heifer carcasses of the normal genotype had more marbling and higher quality grades than carcasses produced by heterozygous heifers, whereas carcasses from heterozygous and normal bulls did not differ in marbling score or quality grade.

Liboriussen (1982)Go compared carcass traits of young crossbred bulls produced by double-muscled (Piedmontese and Belgian Blue), "muscular" Charolais and normal (Angus, Gelbvieh, South Devon, Braunvieh and West Flemish Red) sires. Bulls produced by double-muscled sires had larger longissimus areas and less fat thickness than did bulls produced by normal sires. Bulls produced by Piedmontese and Belgian Blue sires had similar values for longissimus area and fat thickness. Charolais crosses had intermediate values for longissimus area but were similar to double-muscled crosses with respect to fat thickness. Bonsembiante et al. (1975)Go found that F1 Piedmontese bulls had larger longissimus areas than did F1 bulls produced by Red Pied, Romagnola, Chianina and Marchigiana sires. No information concerning carcass quality grade traits of Piedmontese cattle could be found.

Carcass Composition and Partitioning of Carcass Fat.

Results from analysis of variance for proportions of separable carcass components are presented in Table 3Go. Percentages of separable muscle, bone and fat and carcass muscle-to-bone ratio were influenced by sire breed, but not by dam breed type.


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TABLE 3. MEAN SQUARES FOR CARCASS COMPOSITION AND PARTITIONING OF CARCASS FAT
 
Comparisons of sire-group means for percentages and ratios of separable carcass components (Table 2Go) revealed substantial differences among the sire breed groups in carcass fatness and muscularity. Group differences in carcass fatness are reflected by means for separable carcass fat percentage, and contrasts of carcass muscularity are most effectively evaluated using means for muscle-to-bone ratio. Steers sired by P and G bulls produced carcasses with similar percentages of separable fat; both groups had lower (P < .05) carcass fat percentages than RA-sired steers (Table 2Go). Differences in muscle-to-bone ratio favored (P < .05) the P-sired steers. Steers by G and RA sires produced carcasses with similar muscle-to-bone ratios (Table 2Go).

Differences among sire groups in percentages of separable bone (G > P) and muscle (P > G > RA) reflected the variation in fatness and muscularity discussed above. Bone percentage normally is inversely related to fat percentage and muscle-to-bone ratio, whereas muscle percentage is negatively correlated with fat percentage and positively correlated with muscle-to-bone ratio (Berg and Butterfield, 1966Go). Due to a superior combination of leanness and muscularity, P-sired steers had 2.4% and 6.5% higher yields of separable muscle than did G- and RA-sired steers, respectively.

Significant effects of days on feed observed for percentages of separable carcass components (Table 3Go) reflected changes in carcass composition associated with variation in length of the concentrate feeding period. Increased time on feed was associated with an increase in separable fat percentage and decreases in separable muscle and bone percentages. The fact that muscle-to-bone ratio was not affected (P > .05) by length of the feeding period (Table 2Go) indicated that the primary influence of days on feed was exerted on the fat component of the carcass. Moreover, the lack of significance for the interaction between sire breed and days on feed indicated that compositional changes during finishing occurred at similar rates for P-, and G- and RA-sired steers.

Siccardi (1973)Go reported a limited amount of information from three different crossbreeding studies concerning carcass composition of F1 Piedmontese cattle. These studies, which were described earlier, involved comparisons of F1 Piedmontese with purebred Dutch Friesian, Valdostana and Brown Swiss calves. Carcasses from the F1 Piedmontese consistently yielded higher percentages of muscle (from 4.6 to 5.7% higher) and lower percentages of fat (from .7 to 1.5% lower) and bone (from 2.8 to 5.3% lower) than did their purebred contemporaries. Muscle-to-bone ratios were from 1.1 to 2.3 units higher for the F1 calves.

Bonsembiante et al. (1975)Go compared carcass composition of F1 bulls produced by Piedmontese (P), Red Pied (RP), Chianina (C), Marchigiana (M) and Romagnola (R) sires at a constant slaughter weight of 500 kg. Carcasses produced by F1 Piedmontese yielded more (P < .01) lean (P = 71.97%, RP = 63.15%, C = 62.91%, M = 64.63%, R = 64.12%) and less (P < .05) fat (P = 12.47%, RP = 21.67%, C = 20.08%, M = 19.67%, R = 19.35%) than carcasses produced by the four other breed groups. In a similar study, Borghese et al. (1978)Go compared carcass composition yields of F1 bulls produced by mating Piedmontese (P), Chianina (C), Marchigiana (M), Limousin (L) and Charolais (Ch) bulls to Friesian cows. Piedmontese-sired bulls had the highest muscle percentage (P = 71.35%, C = 66.58%, M = 68.18%, L = 67.55%, Ch = 66.56%) and the highest muscle-to-bone ratio (P = 3.94, C = 3.07, M = 3.32, L = 3.52, Ch = 3.32).

Variance components and least squares means for relative proportions of separable carcass fat partitioned into the subcutaneous, intermuscular and internal depots are presented in Tables 2Go and 3Go. Sire breed had no effect (P > .05) on partitioning of separable carcass fat, indicating that the differences observed for fat thickness and percentage of total separable fat were associated with differences in overall degree of carcass fatness and not with differences in partitioning of fat into the different carcass depots. Other reports of fat partitioning data for F1 Piedmontese cattle could not be found.

Palatability Attributes, Collagen Content and Proximate Composition of the Longissimus.

Sire breed was a significant source of variation in shear force and sensory panel ratings for myofibrillar tenderness, flavor intensity and connective tissue amount (Table 4Go). Longissimus steaks from carcasses produced by P- and RA-sired steers received higher (P < .05) ratings for myofibrillar tenderness, flavor intensity and connective tissue amount than did steaks produced by G-sired steers (Table 5Go). Additionally, steaks from RA-sired steers had lower shear force values than did steaks from steers by G sires. Longissimus samples from steers in the three sire breed groups did not differ (P > .05) in intramuscular collagen content or moisture percentage. However, longissimus samples from RA-sired steers had the highest percentage of intramuscular lipid (Table 5Go). Sensory panel ratings for myofibrillar tenderness and connective tissue amount increased and shear force values decreased as time on feed increased (Table 4Go). Collagen content of the longissimus was not affected (P > .05) by days on feed; however, percentage of moisture decreased (P < .01) and percentage of intramuscular lipid tended to increase (P = .10) with increased time on feed (Table 4Go).


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TABLE 4. MEAN SQUARES FOR PALATABILITY ATTRIBUTES, COLLAGEN CONTENT AND PROXIMATE COMPOSITION OF SAMPLES FROM THE LONGISSIMUS MUSCLE
 

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TABLE 5. AGE-CONSTANT LEAST SQUARES MEANS SHOWING THE EFFECTS OF SIRE BREED ON PALATABILITY ATTRIBUTES, COLLAGEN CONTENT, PROXIMATE COMPOSITION AND MUSCLE FIBER PROFILES OF SAMPLES FROM THE LONGISSIMUS MUSCLE
 
Previous research has shown that double-muscled animals produce meat that is more tender than meat from normal animals (Bouton et al., 1982Go). Lawrie et al. (1964)Go, Boccard (1981)Go and Bailey et al. (1982)Go have attributed the superior tenderness of beef from double-muscled animals primarily to its lower intramuscular collagen content.

West et al. (1973)Go found that steaks from the longissimus and semimembranosus muscles of heterozygous double-muscled steers were more tender than corresponding steaks from normal steers. Carroll et al. (1978)Go compared taste panel evaluations of tenderness for longissimus and semimembranosus samples from heterozygous double-muscled and normal heifers and bullocks. They found that, among bullocks, meat produced by heterozygous double-muscled animals was more tender whereas among heifers, meat from normal animals was more tender.

Borghese et al. (1978)Go compared tenderness of longissimus samples from F1 bulls sired by Chianina, Piedmontese, Marchigiana, Limousin and Charolais bulls. Samples from F1 Piedmontese had the lowest Warner-Bratzler shear force values. Shear force values for the other breed groups were similar.

Muscle Fiber Types.

Sire breed was a significant source of variation in percentages of intermediate and white muscle fibers and in cross-sectional area of red muscle fibers in the longissimus muscle (Table 6Go). Steers sired by P bulls had the highest (P < .05) percentage of white fibers, the lowest (P < .05) percentage of intermediate fibers and the smallest (P < .05) areas of red muscle fibers. Fiber profiles and sizes for G- and RA-sired steers were similar (Table 5Go). Areas of red, intermediate and white muscle fibers increased (P < .05) but the percentage of intermediate fibers decreased as time on feed increased (Table 5Go).


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TABLE 6. MEAN SQUARES FOR MUSCLE FIBER TYPE PERCENTAGES AND CROSS-SECTIONAL AREAS OF MUSCLE FIBERS
 
Holmes and Ashmore (1972)Go studied muscle fiber characteristics of homozygous double-muscled, heterozygous double-muscled and normal calves. Biopsy samples taken from the triceps brachii, cutaneous truncii and semitendonosus muscles at 4, 16 and 26 wk of age showed that homozygous double-muscled calves had smaller red fibers than did normal calves. However, at slaughter (15 mo of age) there was no difference in size of red fibers among the three genotypes. Homozygous doubled-muscled calves had larger white fibers than normal calves at all ages whereas white fibers for heterozygotes were intermediate in size. After 16 wk of age, double-muscled calves had a higher percentage of white fibers than did normal calves, whereas percentages of white fibers were similar for normal and heterozygous double-muscled calves. The presumed conversion of intermediate fibers to white fibers during growth (Ashmore et al., 1972Go) was higher for double-muscled calves. Ashmore et al. (1972)Go theorized that selection for increased muscularity in cattle is associated with an increased capacity for conversion of intermediate muscle fibers to white muscle fibers during development.

West (1974)Go reported data comparing fiber characteristics of homozygous double-muscled heterozygous double-muscled and normal females at 454 kg live weight. In the semimembranosus muscle, homozygous double-muscled heifers had a lower percentage of intermediate fibers, which was attributed to greater conversion of intermediate to white fibers.

Our findings generally are consistent with those of previous studies. In the present study F1 Piedmontese steers had a higher percentage of white fibers and a lower percentage of intermediate fibers compared with the other two sire breed groups. Moreover, the percentage of intermediate fibers decreased for all three breed groups as the steers became older. Greater conversion of intermediate to white fibers for P steers during the growth and finishing periods may be a plausible explanation for their different muscle fiber type profiles.

Comparisons at a Constant Marbling Score.

Least squares means for certain traits adjusted linearly to a constant degree of marbling (Small18) are provided in Table 7Go. Because most cattle currently produced in the U.S. are slaughtered at a low Choice quality grade endpoint, these data have practical implications.


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TABLE 7. LEAST SQUARES MEANS FOR SELECTED TRAITS ADJUSTED TO A CONSTANT MARBLING SCORE8
 
Steers sired by P and G bulls had to be fed for longer periods and to heavier carcass weights to attain a "small" degree of marbling than did RA-sired steers. Additionally, P- and G-sired steers had larger (P < .05) longissimus areas than did RA-sired steers. Carcasses of P-sired steers had the least (P < .05) fat thickness, the lowest (P < .05) numerical yield grades and the highest (P < .05) values for muscle-to-bone ratio. Percentage of separable fat at the same degree of marbling was greatest for RA, intermediate for G and least for P steers. Longissimus steaks from P- and RA-sired steers were comparable with respect to palatability attributes; steaks produced by G-sired steers received the lowest (P < .05) ratings for myofibrillar tenderness, flavor intensity and connective tissue amount.

The most exhaustive comparison of sire breeds used in U.S. crossbreeding systems is the Germ Plasm Evaluation Program (GPE) at the U.S. Meat Animal Research Center. Koch et al. (1976Go, 1979)Go compared F1 steers sired by bulls representing various British (Angus, Hereford, Red Poll, South Devon) and continental European (Charolais, Chianina, Gelbvieh, Limousin, Maine Anjou, Simmental) breeds at a constant marbling score (small). At the same degree of marbling, G and P steers in the present study had carcass weights similar to those of the continental-European crosses in the GPE studies. However, the G and P steers in our study were leaner and had larger ribeyes and lower numerical yield grades than the GPE steers. The RA steers in our study had carcass weights that were similar to the British crosses in the GPE studies, but again, our steers were much leaner and had larger ribeyes and lower yield grades. The GPE studies demonstrated very slight breed differences in muscle-to-bone ratio and no effect of breed on meat palatability, which is in contrast to our findings.


    Implications
 Top
 Abstract
 Introduction
 Materials and Methods
 Results
 Implications
 Literature Cited
 
The Piedmontese breed is unique because of its history of selection for double muscling. Our F1 Piedmontese steers produced lean carcasses with superior muscle yields of beef that was very tender. These findings suggest that Piedmontese sires may have potential for use in crossbreeding systems designed to meet U.S. consumer desires for lean, palatable beef.


    Footnotes
 
1 Appreciation is expressed to Peetz Piedmontese Breeders, Inc., Sidney, NE for financial support of the study and to Piedmonte Ltd., Sterling, CO, Piedmontese Breeding Cooperative, Ltd. of Canada and the U.S. Meat Animal Research Center for their contributions to the study. The authors also would like to acknowledge the assistance of Keith Belk, Dale Blasi, Tom and Lisa Field, John Greathouse, Ronnie Green, Mike Holland. Tom Hook, Ralph Kaehler and Dan and Cindy Lisco in collection of carcass composition data. Back

2 Present address: Heritage Lite Meat Corporation, 5147A 69th, Lubbock, TX 79424. Back

3 Present address: Bryan Foods, P.O. Box 1177, West Point, MS 39773. Back

4 Dept of Anim. Sci. Back

Received for publication May 19, 1989. Accepted for publication August 21, 1989.


    Literature Cited
 Top
 Abstract
 Introduction
 Materials and Methods
 Results
 Implications
 Literature Cited
 


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