HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Google Scholar Articles by Miarelli, M. Articles by Signorelli, F. PubMed PubMed Citation Articles by Miarelli, M. Articles by Signorelli, F.

Differential expression of liver proteins in Chianina and Holstein young bulls

CRA – PCM, Research Centre for Meat Production and Genetic Improvement, Via Salaria, 31 00016 Monterotondo (Rome), Italy

maria.miarelli{at}entecra.it

Abstract

The purpose of this work was to produce and compare liver proteomic maps of cattle breeds that have been selected for different purposes: dairy (Holstein) and beef (Chianina). Liver was chosen because it displays functions of synthesis, homeostasis, excretion, and defense. Two dimensional electrophoresis (2-DE) analysis was performed on bovine livers collected from 4 individuals of each breed to evaluate differential protein expression. We found 9 spots differing in photodensity by 2-fold between the 2 breeds. Spots were analysed through combined MALDI-MS and LC-MS/MS. A search against protein sequences in the NCBI databases indicated that the differentially-expressed proteins correspond to liver-specific enzymes, with a major role in the metabolism of fatty acids, carbohydrates, amino acids, and the urea cycle.

Key Words: cattle breeds • liver • proteome • two-dimensional electrophoresis