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Expression of progesterone receptor related to the polymorphism in the PGR gene in the rabbit reproductive tract

R. Peiró^*, A. Herrler, M. A. Santacreu^*, M. Merchán, M. J. Argente, M. L. García, J. M. Folch and A. Blasco^*

^* Instituto de Ciencia y Tecnología Animal. Universidad Politécnica de Valencia, P.O. Box 22012, 46071 Valencia, Spain , Department of Anatomy and Reproductive Biology, Medical School, RWTH University of Aachen, Wendlingweg 2, 52057 Aachen, Germany , Departament de Ciència Animal i dels Aliments. Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain Departamento de Tecnología Agroalimentaria. Universidad Miguel Hernández de Elche, 03312 Orihuela, Spain

ropeibar{at}dca.upv.es

Abstract

The association of the 2464G>A SNP found in the promoter region of the rabbit progesterone receptor gene with progesterone receptor expression was evaluated by Western blot analysis. This SNP was associated with 2 lines divergently selected for uterine capacity, the High line selected to increase uterine capacity and the Low line selected to decrease it. Two progesterone isoforms were obtained using a commercial monoclonal antibody: the PR-B isoform previously described in rabbits, and the PR-A isoform, not previously described in rabbits. The GG genotype, the genotype more frequent in the High line, showed lower PR-B and PR-A expression than the AA genotype in the oviduct (GG/AA_PR-B = 0.81 and GG/AA_PR-A = 0.73) and uterus (around 0.70 in both isoforms). The GA genotype showed similar PR-A expression in both tissues and also similar PR-B expression in the oviduct to the GG genotype. Conversely, the GG genotype showed lower PR-B expression than the GA genotype in the uterus (GG/GA_PR-B =0.79). Similar expression of both PR isoforms was found in the uterus at d 2 and 3 of gestation; meanwhile, an increase of both isoforms was observed in the oviduct. Similar PR-A expression was observed in the ampulla and isthmus; meanwhile, the PR-B expression in the isthmus was double that in the ampulla.

Key Words: oviduct • PGR genotype • PR expression • rabbits • uterus