J. Anim Sci.
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J. Anim Sci. 2010. 88:421-427. doi:10.2527/jas.2009-1955
© 2010 American Society of Animal Science
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MOLECULAR GENETICS

Expression of progesterone receptor related to the polymorphism in the PGR gene in the rabbit reproductive tract1

R. Peiró*,2, A. Herrler{dagger}, M. A. Santacreu*, M. Merchán{ddagger}, M. J. Argente§, M. L. García§, J. M. Folch{ddagger} and A. Blasco*

* Instituto de Ciencia y Tecnología Animal, Universidad Politécnica de Valencia, PO Box 22012, 46071 Valencia, Spain; and {dagger} Department of Anatomy and Reproductive Biology, Medical School, RWTH University of Aachen, Wendlingweg 2, 52057 Aachen, Germany; and {ddagger} Departament de Ciència Animal i dels Aliments, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain; and § Departamento de Tecnología Agroalimentaria, Universidad Miguel Hernández de Elche, 03312 Orihuela, Spain

2 Corresponding author: ropeibar{at}dca.upv.es

The association of the 2464G > A SNP found in the promoter region of the rabbit progesterone receptor gene with progesterone receptor (PR) expression was evaluated by Western blot analysis. This SNP was associated with 2 lines divergently selected for uterine capacity, the high line selected to increase uterine capacity and the low line selected to decrease uterine capacity. Two progesterone isoforms were obtained using a commercial monoclonal antibody: the PR-B isoform described previously in rabbits, and the PR-A isoform, not described previously in rabbits. The GG genotype, the genotype more frequent in the high line, showed less PR-B and PR-A expression than the AA genotype in the oviduct (GG/AAPR-B = 0.81 and GG/AAPR-A = 0.73) and uterus (around 0.70 in both isoforms). The GA genotype showed similar PR-A expression in both tissues and also similar PR-B expression in the oviduct to the GG genotype. Conversely, the GG genotype showed less PR-B expression than the GA genotype in the uterus (GG/GAPR-B = 0.79). Similar expression of both PR isoforms was found in the uterus at d 2 and 3 of gestation; meanwhile, an increase of both isoforms was observed in the oviduct. Similar PR-A expression was observed in the ampulla and isthmus; meanwhile, the PR-B expression in the isthmus was double that in the ampulla.

Key Words: oviduct • PGR genotype • progesterone receptor expression • rabbit • uterus






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