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This Article Full Text Full Text (PDF) All Versions of this Article: jas.2009-1845v1 87/8/2528    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Google Scholar Articles by Manso Filho, H. C. Articles by Watford, M. PubMed PubMed Citation Articles by Manso Filho, H. C. Articles by Watford, M.

Developmental changes in the concentrations of glutamine and other amino acids in plasma and skeletal muscle of the Standardbred foal¹

H. C. Manso Filho^*,, K. H. McKeever^*, M. E. Gordon^*, H. E. Manso^,, W. S. Lagakos, G. Wu and M. Watford^,2

^* Department of Animal Sciences, Rutgers the State University of New Jersey, New Brunswick 08901; and Department of Animal Sciences, Federal Rural University of Pernambuco, Recife, Pernambuco, Brazil; and Department of Nutritional Sciences, Rutgers the State University of New Jersey, New Brunswick 08901; and Department of Animal Science, Texas A & M University, College Station 77843

² Corresponding author: Watford{at}aesop.rutgers.edu

Glutamine is concentrated within skeletal muscle, where it has been proposed to play a regulatory role in maintaining protein homeostasis. The work presented here addressed the hypothesis that glutamine would be the most abundant free -AA in plasma and skeletal muscle in the foal during the first year of life. Glycine, however, was the most abundant free -AA in plasma at birth and between 3 and 12 mo of age. The concentration of glutamine, the second most abundant AA at birth, increased through the first 7 d (P < 0.05) and then returned to values similar to those at birth. This resulted in glutamine being the most abundant free -AA in plasma from 1 d through 1 mo of age. The most abundant free -AA in skeletal muscle at birth was glutamine, but the concentration fell by more than 50% by d 15 and continued to decrease, reaching about one-third of the original values by 1 yr of age (P < 0.05). Glutamine synthetase was barely detectable in skeletal muscle at birth, but the abundance increased rapidly within 15 d of birth. The concentration of glycine, the second most abundant AA in muscle at birth, decreased by about 40% by d 15 (P < 0.05) and then stabilized at this value throughout the year. In contrast, glutamate, alanine, and serine concentrations, the third, fourth, and fifth most abundant free -AA in muscle at birth, respectively, increased to new stable concentrations between 3 and 6 mo of age (P < 0.05). This resulted in alanine being the most abundant free -AA in skeletal muscle at 12 mo of age, followed by glutamate, glutamine, and glycine. The decrease in intramuscular glutamine content, particularly during the first 2 wk after birth, is not compatible with a regulatory role for glutamine in muscle protein synthesis because it occurred at the time of maximum growth in these animals. The findings that, at certain times of development, glutamine was not the most abundant free -AA in the foal is novel and signifies that intramuscular glutamine may have functions specific to muscle type and mammalian species.

Key Words: amino acid • body composition • glutamine • glutamine synthetase • horse • skeletal muscle