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This Article Free Via Open Access Full Text Full Text (PDF) All Versions of this Article: jas.2008-1708v1 87/8/2519    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Google Scholar Articles by Xu, Y. J. Articles by Xiong, Y. Z. PubMed PubMed Citation Articles by Xu, Y. J. Articles by Xiong, Y. Z.

Differential proteome analysis of porcine skeletal muscles between Meishan and Large White¹

Y. J. Xu^*, M. L. Jin, L. J. Wang^*, A. D. Zhang, B. Zuo^*, D. Q. Xu^*, Z. Q. Ren^*, M. G. Lei^*, X. Y. Mo^*, F. E Li^*, R. Zheng^*, C. Y. Deng^* and Y. Z. Xiong^*,2

^* Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture & Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, 1 Shizishan Street, Wuhan, Hubei, 430070, P. R. China; and Unit of Animal Infectious Diseases, National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, 1 Shizishan Street, Wuhan, Hubei, 430070, P. R. China

² Corresponding author: yongjx81{at}webmail.hzau.edu.cn

Western and indigenous Chinese pig breeds show obvious differences in muscle growth and meat quality; however, the underlying molecular mechanism remains unclear. In this study, proteome analysis of LM between purebred Meishan and Large White pigs was performed by 2-dimensional gel electrophoresis and mass spectrometry. A total of 25 protein spots were differentially expressed in the 2 breeds. The 14 identified proteins could be divided into 4 groups: energy metabolism, defense and stress, myofibrillar filaments, and other unclassified proteins. Quantitative real-time PCR was used to analyze the partly differentially expressed proteins in mRNA level, which revealed a positive correlation between the content of the proteins and their mRNA levels. We also analyzed the mRNA levels of myosin heavy chain isoforms using quantitative real-time PCR. The results indicated that IIa and IIx fibers were elevated in Meishan pigs, whereas the IIb fiber was more highly expressed in Large White pigs. To the best of our knowledge, this was the first proteomics-based investigation of total skeletal muscle protein in different pig breeds, and these results may provide valuable information for understanding the molecular mechanism responsible for breed-specific differences in growth performance and meat quality.

Key Words: breed • muscle • pig • proteome • quantitative real-time polymerase chain reaction • two-dimensional gel electrophoresis