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This Article Full Text Full Text (PDF) All Versions of this Article: jas.2007-0177v1 jas.2007-0177v2 87/2/603    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Zhan, Z. P. Articles by Peng, J. Search for Related Content PubMed PubMed Citation Articles by Zhan, Z. P. Articles by Peng, J.

Duration of feeding linseed diet influences expression of inflammation-related genes and growth performance of growing-finishing barrows¹

Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, P. R. China

² Corresponding author: naturevoice{at}hotmail.com

The aim of the study was to investigate the effects of dietary linseed (rich in n-3 PUFA) on expression of inflammation-related genes and on growth performance of growing-finishing barrows. Two isoenergetic and isonitrogenous diets were formulated, one as the basal diet and the other containing 10% linseed. Twenty-four Landrace x Yorkshire barrows weighing 35 ± 3.7 kg were randomly assigned to 1 of 4 treatment groups, with 6 pigs per group. During the entire experimental period of 90 d, these 4 groups of pigs were first fed the basal diet and then fed the linseed diet for 0, 30, 60, and 90 d before slaughter, respectively. Pig growth; messenger RNA (mRNA) expression of peroxisome proliferator-activated receptor- (PPAR), IL-1β0, IL-6, and tumor necrosis factor- (TNF-); and plasma concentrations of the 3 proinflammatory cytokines were measured and analyzed. Average daily feed intake did not differ among treatment groups (P > 0.05), but ADG (P < 0.05) and G:F (P < 0.01) responded quadratically to the duration of linseed diet feeding, and pigs in the 60-d treatment group had the greatest ADG and G:F. The mRNA expression of PPAR in loin muscle and spleen increased linearly (P < 0.01) with the duration of linseed diet feeding, whereas its expression in adipose tissue was not affected (P = 0.095). Tumor necrosis factor- and IL-6 mRNA expression in muscle, adipose, and spleen, as well as serum concentration of TNF-, decreased linearly (P < 0.01) with the duration of linseed diet feeding. Peroxisome proliferator-activated receptor- mRNA abundance was negatively correlated with IL-1β, IL-6, and TNF- mRNA abundance both in muscle (R² = 0.63, P < 0.001) and in spleen (R² = 0.69, P < 0.001), and PPAR mRNA expression in spleen (R² = 0.59, P < 0.01) and muscle (R² = 0.52, P < 0.05) was negatively correlated with serum TNF- concentration. There were also significant quadratic relations between ADG and expression of PPAR (P < 0.05) and splenic TNF- (P < 0.05). These data suggest that intake of n-3 PUFA from the linseed diet led to significant decreases in the expression of proinflammatory cytokine genes, which may stimulate growth in growing-finishing barrows, at least in part, through a PPAR-dependent mechanism.

Key Words: growth • linseed • n-3 polyunsaturated fatty acid • peroxisome proliferator-activated receptor- • pig • proinflammatory cytokine