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This Article Full Text Full Text (PDF) All Versions of this Article: jas.2009-2068v1 87/11/3544    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Google Scholar Articles by Dan, H. Articles by Monsonego Ornan, E. PubMed PubMed Citation Articles by Dan, H. Articles by Monsonego Ornan, E.

Expression of matrix metalloproteinases during impairment and recovery of the avian growth plate¹

H. Dan^*,,2, S. Simsa-Maziel^*,2, A. Hisdai, D. Sela-Donenfeld and E. Monsonego Ornan^*,3

^* Department of Biochemistry and Nutrition, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University, Israel 76100; and Institute of Animal Science, the Volcani Center, Bet Dagan, Israel 50200; and Koret School of Veterinary Medicine, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University, Israel 76100

³ Corresponding author: ornanme{at}agri.huji.ac.il

Tibial dyschondroplasia (TD) is a prevalent skeletal abnormality associated with rapid growth rate in many avian species. It is characterized by the presence of a nonvascularized, nonmineralized lesion that extends from the epiphyseal growth plate into the metaphysis of the proximal tibiotarsal bones. In this study, we examined the expression of 4 members of the matrix metalloproteinase (MMP) family (MMP-2, -3, -9, and -13) in thiram-induced TD lesions and in the process of recovery from TD, by in situ hybridization analysis and quantitative real-time PCR. A model for the induction and recovery of TD was established, consisting of 3 groups of broilers: (1) thiram group, chicks fed a thiram-enriched diet to induce TD; (2) recovery group, chicks fed a thiram-enriched diet during the first week of the experiment and a normal diet from the second week on; and (3) control group, chicks fed a normal diet throughout the experimental period. In agreement with our previous data, the 4 MMP were diminished in the TD lesion (P < 0.05); however, in the current study we show that the growth plate was able to repair itself and that the MMP reappeared during the process of recovery from TD. Our results strengthen the link between MMP expression and growth-plate impairment, and we suggest that gelatinase activity (MMP-2 and 9) facilitates this process.

Key Words: avian • growth plate • matrix metalloproteinase • tibial dyschondroplasia