HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: jas.2008-0860v1 86/10/2531    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Grant, A. C. Articles by Buskirk, D. D. Search for Related Content PubMed PubMed Citation Articles by Grant, A. C. Articles by Buskirk, D. D.

Differentiation of bovine intramuscular and subcutaneous stromal-vascular cells exposed to dexamethasone and troglitazone¹

A. C. Grant^*,2, G. Ortiz-Colón^*,3, M. E. Doumit^*,, R. J. Tempelman^* and D. D. Buskirk^*,4

^* Departments of Animal Science and and Food Science and Human Nutrition, Michigan State University, East Lansing 48824-1225

⁴ Corresponding author: buskirk{at}msu.edu

The objectives of these experiments were to compare differentiation of bovine stromal-vascular (S-V) cells isolated from i.m. and s.c. adipose tissues in response to a glucocorticoid and a peroxisome proliferator-activated receptor agonist. Stromal-vascular cells were isolated from i.m. and s.c. fat depots of 3 Angus steers and propagated in culture. Cells were exposed to differentiation media containing 0.25 µM dexamethasone (DEX), a glucocorticoid analog, and 40 µM troglitazone (TRO), a peroxisome proliferator-activated receptor agonist, or both. Cells treated with DEX and TRO had greater (P < 0.02) glycerol-3-phosphate dehydrogenase activity than control cells. No interactions between DEX, TRO, and depot (P > 0.59) or depot differences (P = 0.41) in glycerol-3-phosphate dehydrogenase activity were found. Morphological assessment of adipogenic colonies showed that DEX induced a 1.8-fold increase in the percentage of adipogenic colonies (P = 0.03), whereas TRO increased the proportion of adipogenic colonies by 1.9-fold (P = 0.02) compared with those not treated with DEX or TRO, respectively. Depots had a similar percentage of adipogenic colonies (P = 0.18); however, the percentage of differentiated cells within adipogenic colonies was found to be 6.4-fold greater in s.c. isolates compared with i.m. (P < 0.001). Addition of TRO increased the proportion of differentiated cells within colonies by 10-fold compared with those of nontreated colonies (P < 0.001), whereas the percentage of differentiated cells within adipogenic colonies only tended to be increased by DEX (P = 0.10). These data indicate that bovine i.m. and s.c. S-V cells are capable of enhanced differentiation in response to DEX and TRO, and these effects were additive. Most importantly, inherent differences in the capacity to differentiate exist between adipogenic bovine i.m. and s.c. S-V cells.

Key Words: bovine • differentiation • glycerol-3-phosphate dehydrogenase • glucocorticoid • peroxisome proliferator-activated receptor gamma • stromal-vascular

This article has been cited by other articles:

				G. Ortiz-Colon, A. C. Grant, M. E. Doumit, and D. D. Buskirk Bovine intramuscular, subcutaneous, and perirenal stromal-vascular cells express similar glucocorticoid receptor isoforms, but exhibit different adipogenic capacity J Anim Sci, June 1, 2009; 87(6): 1913 - 1920. [Abstract] [Full Text] [PDF]