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This Article Full Text Full Text (PDF) All Versions of this Article: jas.2006-491v1 85/6/1511    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by O’Fallon, J. V. Articles by Gaskins, C. T. Search for Related Content PubMed PubMed Citation Articles by O’Fallon, J. V. Articles by Gaskins, C. T.

A direct method for fatty acid methyl ester synthesis: Application to wet meat tissues, oils, and feedstuffs

Department of Animal Sciences, Washington State University, Pullman 99164

¹ Corresponding author: gaskins{at}wsu.edu

A simplified protocol to obtain fatty acid methyl esters (FAME) directly from fresh tissue, oils, or feedstuffs, without prior organic solvent extraction, is presented. With this protocol, FAME synthesis is conducted in the presence of up to 33% water. Wet tissues, or other samples, are permeabilized and hydrolyzed for 1.5 h at 55°C in 1 N KOH in MeOH containing C13:0 as the internal standard. The KOH is neutralized, and the FFA are methylated by H₂SO₄ catalysis for 1.5 h at 55°C. Hexane is then added to the reaction tube, which is vortex-mixed and centrifuged. The hexane is pipetted into a gas chromatography vial for subsequent gas chromatography. All reactions are conducted in a single screw-cap Pyrex tube for convenience. The method meets many criteria for fatty acid analysis, including not isomerizing CLA or introducing fatty acid artifacts. It is applicable to fresh, frozen, or lyophilized tissue samples, in addition to oils, waxes, and feedstuffs. The method saves time and effort and is economical when compared with other methods. Its unique performance, including easy sample preparation, is achieved because water is included rather than eliminated in the FAME reaction mixtures.

Key Words: conjugated linoleic acid • fatty acid analysis • fatty acid methyl ester synthesis • feedstuff • fish oil • longissimus muscle

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