HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: jas.2006-524v1 85/5/1126    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Singh, N. K. Articles by Chung, H. Y. Search for Related Content PubMed PubMed Citation Articles by Singh, N. K. Articles by Chung, H. Y.

Transdifferentiation of porcine satellite cells to adipoblasts with ciglitizone¹

N. K. Singh, H. S. Chae^,2, I. H. Hwang^*, Y. M. Yoo, C. N. Ahn, S. H. Lee, H. J. Lee, H. J. Park and H. Y. Chung^||

^* Department of Animal Resources and Biotechnology, Chonbuk National University, Chonju, Republic of Korea; and and Product and Utility Division, and Functional Animal Genomics, and Nutrition and Physiology, and and ^|| Animal Genomics and Bioinformatics Division, National Livestock Research Institute, Suwon 441-350, Republic of Korea

² Corresponding author: hs6226{at}rda.go.kr

Ciglitizone, a class of thiazolidinediones, acts as a potent activator of the adipose differentiation program in established preadipose cell lines. Thiazolidinediones have also been investigated in diabetic patients and have been reported to act as peroxisome proliferator-activated receptor- ligands. Intramuscular adipogenesis or marbling through transdifferentiation of satellite cells in cattle was successfully conducted earlier. In this report, the effects of ciglitizone on the differentiation pathway of porcine myogenic satellite cells was investigated. Semitendinosus muscle was aseptically taken from 10-d-old piglets under general anesthesia, and porcine satellite cells were obtained and grown to near confluence. Postconfluent cells (d 0) were further cultured in differentiation medium containing an adipogenic mixture plus ciglitizone (10 µM) for 48 h. From d 2 onward, the cells were cultured only in the presence of ciglitizone until d 10. Controls were cultured in differentiation medium only. Exposure of porcine satellite cells to the adipogenic mixture plus ciglitizone generated lipid droplets on d 2, and subsequently, exposure of cells to ciglitizone alone helped in cytoplasmic lipid filling, providing them with the acquisition of adipocyte morphology. An increase (P < 0.05) in the fusion (structures containing 2 to 3 nuclei) of satellite cells was observed, and myosin heavy chain appeared with greater intensity (immunohistochemistry) in the control group from d 2 onward. Adipocyte-specific transcriptional factors (i.e., CCAAT/enhancer binding protein- and peroxisome proliferator-activated receptor-) were predominant during transdifferentiation and were observed with immuhistochemistry, Western blot (~47.2 and ~60.4 kDa, respectively), and real-time PCR. Ciglitizone appeared to convert the differentiation pathway of satellite cells into that of adipoblasts.

Key Words: adipogenesis • ciglitizone • CCAAT/enhancer binding protein- • myogenesis • peroxisome proliferator-activated receptor-

This article has been cited by other articles:

				G. J. Hausman, S. P. Poulos, T. D. Pringle, and M. J. Azain The influence of thiazolidinediones on adipogenesis in vitro and in vivo: Potential modifiers of intramuscular adipose tissue deposition in meat animals J Anim Sci, April 1, 2008; 86(14_suppl): E236 - E243. [Abstract] [Full Text] [PDF]

				K. Y. Chung and B. J. Johnson Application of cellular mechanisms to growth and development of food producing animals J Anim Sci, April 1, 2008; 86(14_suppl): E226 - E235. [Abstract] [Full Text] [PDF]