HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: jas.2006-278v1 85/4/886    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Zhang, F. W. Articles by Lei, M. G. Search for Related Content PubMed PubMed Citation Articles by Zhang, F. W. Articles by Lei, M. G.

Imprinted status of pleomorphic adenoma gene-like I and paternal expression gene 10 genes in pigs¹

F. W. Zhang^*, H. C. Cheng^*, C. D. Jiang, C. Y. Deng^*,2, Y. Z. Xiong^*, F. E. Li^* and M. G. Lei^*

^* Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China; and and Department of Bioengineering, College of Animal Sciences, Southwest University, Chongqing 400716, China

² Corresponding author: 030860005{at}webmail.hzau.edu.cn

Genomic imprinting is theorized to exist in all placental mammals and some marsupials. Imprinted genes play important roles in the regulation of fetal growth, development, and postnatal behavior, but the study of imprinted genes has been limited in livestock. In this study, the polymorphism-based approach was used to detect the expression patterns of the porcine pleomorphic adenoma gene-like I (PLAGL1) and paternal expression gene 10 (PEG10) genes. Single nucleotide polymorphisms in the exons were detected between the Meishan and Large White breeds in the PLAGL1 and PEG10 genes. The polymorphisms were used to determine the monoallelic or biallelic expression with reverse transcription-PCR-RFLP in 44 tissues from 4 heterozygous pigs (based on SNP). Imprinting analysis indicated that the PLAGL1 and PEG10 genes were both paternally expressed in all tissues tested (heart, liver, spleen, lung, kidney, stomach, small intestine, skeletal muscle, fat, uterus, and ovary). Our study showed that the method of identifying polymorphic transcripts with reverse transcription-PCR-RFLP may be beneficial for detecting the imprinting status of some candidate imprinted genes.

Key Words: imprinting • paternal expression gene 10 • pig • pleomorphic adenoma gene-like I

This article has been cited by other articles:

				M. B. Clark, M. Janicke, U. Gottesbuhren, T. Kleffmann, M. Legge, E. S. Poole, and W. P. Tate Mammalian Gene PEG10 Expresses Two Reading Frames by High Efficiency 1 Frameshifting in Embryonic-associated Tissues J. Biol. Chem., December 28, 2007; 282(52): 37359 - 37369. [Abstract] [Full Text] [PDF]