HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: jas.2006-535v1 85/3/673    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Pié, S. Articles by Oswald, I. P. Search for Related Content PubMed PubMed Citation Articles by Pié, S. Articles by Oswald, I. P.

Effects of added fermentable carbohydrates in the diet on intestinal proinflammatory cytokine-specific mRNA content in weaning piglets¹

S. Pié^*,2, A. Awati^,2,3, S. Vida^*, I. Falluel^*, B. A. Williams^,5 and I. P. Oswald^*

^* Institut National de la Recherche Agronomique, Unité de Pharmacologie-Toxicologie, UR66, 180 chemin de Tournefeuille, 31931 Toulouse Cedex 9, France; and Animal Nutrition Group, Wageningen University and Research Centre, PO Box 338, 6700 AH, Wageningen, the Netherlands

⁵ Corresponding author: ioswald{at}toulouse.inra.fr

There is increasing evidence showing that dietary supplementation with prebiotics can be effective in the treatment of intestinal inflammation. Because weaning time is characterized by rapid intestinal inflammation, this study investigated the effect of a diet supplemented with a combination of 4 fermentable carbohydrates (lactulose, inulin, sugarbeet pulp, and wheat starch) on the mRNA content of proinflammatory cytokines in newly weaned piglets. Cytokines (IL-1ß, IL-6, IL-8, IL-12p40, IL-18, and tumor necrosis factor-) were analyzed using a semiquantitative reverse-transcription PCR technique on d 1, 4, and 10 in the ileum and colon of piglets fed either a test diet (CHO) or a control diet. In addition to the diet, the effect of enforced fasting on cytokine mRNA content was also evaluated. No effect of fasting was observed on the pro-inflammatory cytokine mRNA content. Our results showed that the CHO diet induced an up-regulation of IL-6 mRNA content in the colon of piglets 4 d postweaning. This up-regulation was specific for the animals fed the CHO diet and was not observed in animals fed the control diet. An increase in IL-1ß mRNA content was also observed on d 4 postweaning in all of the piglets. Correlations between proinflammatory cytokines and the end-products of fermentation indicated that the regulation of cytokines may be linked with some of the fermentation end-products such as branched-chain fatty acids, which are in turn end-products of protein fermentation.

Key Words: pig • prebiotic • proinflammatory cytokine • weaning

This article has been cited by other articles:

				T. E. Weber, C. J. Ziemer, and B. J. Kerr Effects of adding fibrous feedstuffs to the diet of young pigs on growth performance, intestinal cytokines, and circulating acute-phase proteins J Anim Sci, April 1, 2008; 86(4): 871 - 881. [Abstract] [Full Text] [PDF]