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This Article Full Text Full Text (PDF) All Versions of this Article: jas.2006-237v1 85/10/2517    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Albin, D. M. Articles by Nowak, R. A. Search for Related Content PubMed PubMed Citation Articles by Albin, D. M. Articles by Nowak, R. A.

Changes in small intestinal nutrient transport and barrier function after lipopolysaccharide exposure in two pig breeds¹

D. M. Albin^*,2,3, J. E. Wubben^,2, J. M. Rowlett, K. A. Tappenden^* and R. A. Nowak^,4

^* Division of Nutritional Sciences, and and Department of Animal Sciences, University of Illinois, Urbana 61801

⁴ Corresponding author: ranowak{at}uiuc.edu

Specific pig breeds with unique characteristics have been developed, and the current study sought to characterize some of these differences. Using modified Ussing chambers, electrophysiological mucosal transport of D-glucose, L-Gln, L-Pro, L-Arg, L-Thr, and glycylsarcosine was assessed in small intestinal tissues (duodenum, jejunum, ileum) taken from Yorkshire-based hybrid (BW = 142.4 ± 2.0 kg; mean age = 8 mo) and Meishan (BW = 65.8 ± 0.8 kg; mean age = 6 mo) female pigs after 4 h of lipopolysaccharide (LPS) exposure. Gilts were randomly assigned to control (saline infusion; n = 6 Yorkshires, n = 5 Meishans) or LPS (n = 7 Yorkshires, n = 5 Meishans) groups. Therefore, treatments were arranged in a 2 (breed) x 2 (LPS infusion) factorial. Four hours after infusions, pigs were euthanized, and intestinal segment samples were removed. Glucose transport in the ileum was decreased (P < 0.001) in Yorkshires with LPS but was increased (P < 0.001) by over 2-fold in Meishans with LPS. After LPS infusion, Pro transport was increased in duodenum (over 5-fold; P = 0.04) and ileum (over 10-fold; P < 0.001) of Meishans but was unaffected in Yorkshires. Arginine transport in the ileum of control Meishans was greater (P = 0.05) than Arg transport in control Yorkshires. Glycylsarcosine transport was greater (P = 0.02) in Meishans than Yorkshires (nearly 2-fold), regardless of LPS provision. Glycylsarcosine transport was increased (P = 0.003) over 2-fold by LPS, regardless of pig breed. Resistance (barrier function) was increased (P = 0.03) by LPS in Yorkshires but was unaffected in Meishans. The current study indicates that small intestinal function responded differently to LPS in Yorkshire and Meishan gilts and that these effects were nutrient- and segment-dependent.

Key Words: nutrient transport • barrier function • intestine • lipopolysaccharide • pig breed