| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
ANIMAL GROWTH, PHYSIOLOGY, AND REPRODUCTION |
* USDA-ARS, Russell Agricultural Research Center, Athens, GA 30604;
and
Animal Physiology Unit, University of Georgia, Athens 30605;
and
Pennington Biomedical Research Center, Baton Rouge, LA 70808
2 Corresponding author: ghausman{at}saa.ars.usda.gov
Although microarray and proteomic studies have indicated the expression of unique and unexpected genes and their products in human and rodent adipose tissue, similar studies of meat animal adipose tissue have not been reported. Thus, total RNA was isolated from stromal-vascular (S-V) cell cultures (n = 4; 2 arrays; 2 cultures/array) from 90-d (79% of gestation) fetuses and adipose tissue from 105-d (92% of gestation) fetuses (n = 2) and neonatal (5-d-old) pigs (n = 2). Duplicate adipose tissue microarrays (n = 4) represented RNA samples from a pig and a fetus. Dye-labeled cDNA probes were hybridized to custom microarrays (70-mer oligonucleotides) representing more than 600 pig genes involved in growth and reproduction. Microarray studies showed significant expression of 40 genes encoding for known adipose tissue secreted proteins in fetal S-V cell cultures and adipose tissue. Expression of 10 genes encoding secreted proteins not known to be expressed by adipose tissue was also observed in neonatal adipose tissue and fetal S-V cell cultures. Additionally, the agouti gene was detected by reverse transcription-PCR in pig S-V cultures and adipose tissue. Proteomic analysis of adipose tissue and fetal and young pig S-V cell culture-conditioned media identified multiple secreted proteins including heparin-like epidermal growth factor-like growth factor and several apolipoproteins. Another adipose tissue secreted protein, plasminogen activator inhibitor-1, was identified by ELISA in S-V cell culture media. A group of 20 adipose tissue secreted proteins were detected or identified using the gene microarray and the proteomic and protein assay approaches including apolipoprotein-A1, apolipoprotein-E, relaxin, brain-derived neurotrophic factor, and IGF binding protein-5. These studies demonstrate, for the first time, the expression of several major secreted proteins in pig adipose tissue that may influence local and central metabolism and growth.
Key Words: adipose tissue • cell culture • fat cell • gene microarray • proteomics • secreted factor
This article has been cited by other articles:
|
|
 |
|
  F. Gondret, N. Guitton, C. Guillerm-Regost, and I. Louveau Regional differences in porcine adipocytes isolated from skeletal muscle and adipose tissues as identified by a proteomic approach J Anim Sci, September 1, 2008; 86(9): 2115 - 2125. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Davoli and S. Braglia Molecular approaches in pig breeding to improve meat quality Brief Funct Genomic Proteomic, January 21, 2008; (2008) elm036v1. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. J. Hausman, C. R. Barb, and R. G. Dean Patterns of gene expression in pig adipose tissue: Transforming growth factors, interferons, interleukins, and apolipoproteins J Anim Sci, October 1, 2007; 85(10): 2445 - 2456. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Gimble, A. J. Katz, and B. A. Bunnell Adipose-Derived Stem Cells for Regenerative Medicine Circ. Res., May 11, 2007; 100(9): 1249 - 1260. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
