| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
ANIMAL NUTRITION |
Department of Animal Health, Welfare and Nutrition, Danish Institute of Agricultural Sciences, Research Centre Foulum, DK-8830 Tjele, Denmark
2 Correspondence; P.O. Box 50 (phone: +45 89 99 11 18; fax: +45 89 99 13 78; e-mail:mette.hedemann{at}agrsci.dk).
A 2 x 2 factorial experiment with pigs was undertaken to investigate the effect of particle size (fine and coarse) and feed processing (pelleted and nonpelleted) on morphological characteristics in the small intestine, cecum, and colon of pigs and on the adhesion of Salmonella enterica serovar Typhimurium DT12 to the ileum in vitro. Ninety-six pigs (average BW = 33 ± 7 kg) were fed the experimental diets. After 4 wk, 24 pigs were selected (six pigs per diet) and euthanized, and tissue samples were taken from the mid and distal small intestine, cecum, and distal colon. The effects of particle size and feed processing on villus height and crypt depth in the small intestine were minor. Feeding coarse diets increased (P = 0.05) the crypt depth in the colon. The crypt depth was 420 ± 12 and 449 ± 12 µm in pigs fed finely and coarsely ground feed, respectively. Pigs fed pelleted diets had a larger (P = 0.01) staining area for neutral mucins, as well as for acidic and sulfomucins on the villi of the distal small intestine than pigs fed nonpelleted diets. The area was 41, 46, and 33% larger for neutral, acidic, and sulfomucins, respectively. The mucin-staining areas of the crypts in the cecum and the colon were not affected by the experimental diets. Examination of lectin binding characteristics of the distal small intestine and the cecum did not reveal any differences between the experimental diets. Using a pig intestine organ culture model, Salmonella adhered less (P < 0.05) to the ileal tissue of pigs fed the nonpelleted diets than to those fed pelleted diets; the adherence was 60% less in these pigs. Results of this study suggest that pigs fed pelleted diets secrete mucins that are capable of binding Salmonella enterica serovar Typhimurium DT12 and thereby allowing for colonization. Therefore, pigs fed a nonpelleted diet are better protected against Salmonella infections than pigs fed a pelleted diet.
Key Words: Morphology • Mucin • Particle Size • Pig • Salmonella
This article has been cited by other articles:
|
|
 |
|
  M. S. Hedemann, B. B. Jensen, and H. D. Poulsen Influence of dietary zinc and copper on digestive enzyme activity and intestinal morphology in weaned pigs J Anim Sci, December 1, 2006; 84(12): 3310 - 3320. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Lesniewska, I. Rowland, P. D. Cani, A. M. Neyrinck, N. M. Delzenne, and P. J. Naughton Effect on Components of the Intestinal Microflora and Plasma Neuropeptide Levels of Feeding Lactobacillus delbrueckii, Bifidobacterium lactis, and Inulin to Adult and Elderly Rats. Appl. Envir. Microbiol., October 1, 2006; 72(10): 6533 - 6538. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. S. Hedemann, M. Eskildsen, H. N. Laerke, C. Pedersen, J. E. Lindberg, P. Laurinen, and K. E. B. Knudsen Intestinal morphology and enzymatic activity in newly weaned pigs fed contrasting fiber concentrations and fiber properties J Anim Sci, June 1, 2006; 84(6): 1375 - 1386. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V Lesniewska, I Rowland, H. N Laerke, G Grant, and P. J Naughton Relationship between dietary-induced changes in intestinal commensal microflora and duodenojejunal myoelectric activity monitored by radiotelemetry in the rat in vivo Exp Physiol, January 1, 2006; 91(1): 229 - 237. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
