| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
ANIMAL GROWTH, PHYSIOLOGY, AND REPRODUCTION |
ARS, USDA Richard B. Russell Agricultural Research Center, Animal Physiology Research Unit, Athens, GA 30605-2720
2 Correspondence: 950 College Station Rd. (phone: 706-583-8275; fax: 706-542-0399; e-mail: ghausman{at}saa.ars.usda.gov).
The relationships between adipocyte and muscle cell development within muscle are important in the study of factors or agents that may improve meat quality. Neonatal porcine muscle has the potential to yield both cell types for cell culture because it contains developing adipocytes and a high number of muscle satellite cells. Therefore, we modified a conventional collagenase-based procedure to digest neonatal porcine muscle and subsequently cultured the resultant muscle stromal-vascular (SV) cells on several substrata in basal and dexamethasone (DEX)-containing media. Developing myotubes and preadipocytes were present in muscle SV cell cultures on laminin substrata following seeding and plating with fetal bovine serum (FBS) with or without DEX. Myotube number was much higher (P < 0.05) on laminin substrata compared with all other substrata, whereas preadipocyte number in muscle SV cell cultures was independent of substrata, as we have shown previously. This approach can be used to establish co-cultures of differentiating adipocytes and myotubes from collagenase-digested neonatal pig muscle. Because the comparison is within the same culture dish, this method allows for a direct comparison of the responses of adipogenic and myogenic cells to growth and differentiation factors. For example, DEX did not alter myogenesis (i.e., 11 ± 3 vs. 11 ± 4 myotubes per unit area for control and DEX-treated cultures, respectively), but it has been shown to markedly increase preadipocyte number in muscle SV cell cultures.
Key Words: Cell Biology • Cell Culture • Laminin • Myotube • Preadipocyte
This article has been cited by other articles:
|
|
 |
|
  L Bouraoui, J Gutierrez, and I Navarro Regulation of proliferation and differentiation of adipocyte precursor cells in rainbow trout (Oncorhynchus mykiss) J. Endocrinol., September 1, 2008; 198(3): 459 - 469. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. J. Hausman, S. P. Poulos, T. D. Pringle, and M. J. Azain The influence of thiazolidinediones on adipogenesis in vitro and in vivo: Potential modifiers of intramuscular adipose tissue deposition in meat animals J Anim Sci, April 1, 2008; 86(14_suppl): E236 - E243. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Zhou, D. Li, J. Yin, J. Ni, B. Dong, J. Zhang, and M. Du CLA differently regulates adipogenesis in stromal vascular cells from porcine subcutaneous adipose and skeletal muscle J. Lipid Res., August 1, 2007; 48(8): 1701 - 1709. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Li, H. N. Zerby, and K. Lee Heart fatty acid binding protein is upregulated during porcine adipocyte development J Anim Sci, July 1, 2007; 85(7): 1651 - 1659. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. P. Poulos and G. J. Hausman A comparison of thiazolidinedione-induced adipogenesis and myogenesis in stromal-vascular cells from subcutaneous adipose tissue or semitendinosus muscle of postnatal pigs J Anim Sci, May 1, 2006; 84(5): 1076 - 1082. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
