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Luteinizing hormone-releasing hormone fusion protein vaccines block estrous cycle activity in beef heifers¹

J. D. Stevens^*,, J. M. Sosa^*,, D. M. deAvila^*,, J. M. Oatley^*,, K. P. Bertrand, C. T. Gaskins^* and J. J. Reeves^*,,2

^* Department of Animal Sciences, and Center for Reproductive Biology and and School of Molecular Biosciences, Washington State University, Pullman 99164

² Correspondence: 220 Clark Annex (phone: 509-335-8339; fax: 509-335-4246; e-mail: reevesjj{at}wsu.edu).

Two LHRH fusion proteins, thioredoxin and ovalbumin, each containing seven LHRH inserts were tested for their ability to inhibit estrous cycle activity. The objective was to evaluate immune and biological responses from alternating the two fusion proteins in an immunization schedule. One hundred ten heifers were divided equally into 11 groups. Two control groups consisted of either spayed or intact, untreated heifers. Heifers in the other nine groups were immunized on wk 0, 4, and 9. Treatments were immunizations of the same protein throughout or alternating the proteins in different booster sequences. Blood was collected weekly for 22 wk, and serum was assayed for concentrations of progesterone and titers of anti-LHRH. At slaughter, reproductive tracts were removed from each heifer and weighed. Heifers with 1 ng/mL of progesterone were considered to have a functional corpus luteum and thus to have estrous cycle activity. All LHRH-immunized groups of heifers had a smaller (P < 0.05) proportion of heifers showing estrous cycle activity after 6 wk than the intact, untreated control group. There was no difference in number of heifers cycling between the immunized groups and the spayed heifers during wk 9 to 22. Anti-LHRH did not differ among immunized groups during wk 1 to 9. Starting at wk 10 and continuing through the conclusion of the study, there was an overall difference among treatment groups for anti-LHRH (P < 0.05). Uterine weights differed among treatments (P < 0.05), with intact control animals having heavier uteri than all other groups (P < 0.05). Uterine weights were negatively correlated with maximum LHRH antibody binding (r = –0.44). In summary, the LHRH fusion proteins were as effective as surgical spaying in suppression of estrous cycle activity, but alternating the two proteins in an immunization schedule did not enhance the immunological or biological effectiveness of the vaccine.

Key Words: Fusion Protein • Heifers • Immunization • Luteinizing Hormone-Releasing Hormone

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