| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
ANIMAL GENETICS |
* Cooperative Research Centre for Cattle and Beef Quality, CSIRO Livestock Industries, Queensland Bioscience Precinct, St. Lucia, Queensland 4067, Australia; and
and
Food Science Australia, Tingalpa DC, Queensland 4173, Australia
2 Correspondence: 306 Carmody Rd. (phone: +61-7-3214-2392; fax: +61-7-3214-2900; e-mail: Tony.Reverter-Gomez{at}csiro.au).
Expression profiling using microarrays allows for the detailed characterization of the gene networks that regulate an animal’s response to environmental stresses. During nutritional restriction, processes such as protein turnover, connective tissue remodeling, and muscle atrophy take place in the skeletal muscle of the animal. These processes and their regulation are of interest in the context of managing livestock for optimal production efficiency and product quality. Here we expand on recent research applying complementary DNA (cDNA) microarray technology to the study of the effect of nutritional restriction on bovine skeletal muscle. Using a custom cDNA microarray of 9,274 probes from cattle muscle and s.c. fat libraries, we examined the differential gene expression profile of the LM from 10 Brahman steers under three different dietary treatments. The statistical approach was based on mixed-model ANOVA and model-based clustering of the BLUP solutions for the gene x diet interaction effect. From the results, we defined a transcript profile of 156 differentially expressed array elements between the weight loss and weight gain diet substrates. After sequence and annotation analyses, the 57 upregulated elements represented 29 unique genes, and the 99 downregulated elements represented 28 unique genes. Most of these co-regulated genes cluster into groups with distinct biological function related to protein turnover and cytoskeletal metabolism and contribute to our mechanistic understanding of the processes associated with remodeling of muscle tissue in response to nutritional stress.
Key Words: Beef • Complementary DNA • Gene Expression • Nutrition
This article has been cited by other articles:
|
|
 |
|
  E. E. Dupont-Versteegden, R. Nagarajan, M. L. Beggs, E. D. Bearden, P. M. Simpson, and C. A. Peterson Identification of cold-shock protein RBM3 as a possible regulator of skeletal muscle size through expression profiling Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2008; 295(4): R1263 - R1273. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Ollier, C. Robert-Granie, L. Bernard, Y. Chilliard, and C. Leroux Mammary Transcriptome Analysis of Food-Deprived Lactating Goats Highlights Genes Involved in Milk Secretion and Programmed Cell Death J. Nutr., March 1, 2007; 137(3): 560 - 567. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. P. Mane, C. Vasquez-Robinet, A. A. Sioson, L. S. Heath, and R. Grene Early PLD{alpha}-mediated events in response to progressive drought stress in Arabidopsis: a transcriptome analysis J. Exp. Bot., January 1, 2007; 58(2): 241 - 252. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Salem, P. B. Kenney, C. E. Rexroad 3rd, and J. Yao Microarray gene expression analysis in atrophying rainbow trout muscle: a unique nonmammalian muscle degradation model Physiol Genomics, December 13, 2006; 28(1): 33 - 45. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Reverter, N. J. Hudson, Y. Wang, S.-H. Tan, W. Barris, K. A. Byrne, S. M. McWilliam, C. D. K. Bottema, A. Kister, P. L. Greenwood, et al. A gene coexpression network for bovine skeletal muscle inferred from microarray data Physiol Genomics, December 13, 2006; 28(1): 76 - 83. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. A. Lehnert, K. A. Byrne, A. Reverter, G. S. Nattrass, P. L. Greenwood, Y. H. Wang, N. J. Hudson, and G. S. Harper Gene expression profiling of bovine skeletal muscle in response to and during recovery from chronic and severe undernutrition J Anim Sci, December 1, 2006; 84(12): 3239 - 3250. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Reecy, D. M. Spurlock, and C. H. Stahl Gene expression profiling: Insights into skeletal muscle growth and development J Anim Sci, April 1, 2006; 84(13_suppl): E150 - E. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Reverter, W. Barris, S. McWilliam, K. A. Byrne, Y. H. Wang, S. H. Tan, N. Hudson, and B. P. Dalrymple Validation of alternative methods of data normalization in gene co-expression studies Bioinformatics, April 1, 2005; 21(7): 1112 - 1120. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
