HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Mao, J. Articles by Day, B. N. Search for Related Content PubMed PubMed Citation Articles by Mao, J. Articles by Day, B. N.

Effect of epidermal growth factor and insulin-like growth factor I on porcine preantral follicular growth, antrum formation, and stimulation of granulosal cell proliferation and suppression of apoptosis in vitro¹

J. Mao^*, M. F. Smith^*, E. B. Rucker^*, G. M. Wu^*, T. C. McCauley^*, T. C. Cantley^*, R. S. Prather^*, B. A. Didion and B. N. Day^*,2

^* Department of Animal Sciences, University of Missouri-Columbia, Columbia 65211; and and Monsanto, St. Louis, MO 63198

² Correspondence: 159 Animal Science Research Center, 920 East Campus Dr. (phone: 573-882-7555; fax: 573-884-7827; e-mail: DayB{at}missouri.edu).

The object of this study was to investigate the role of epidermal growth factor (EGF) and IGF-I in the regulation of preantral follicular growth, antrum formation, and granulosal cell proliferation/apoptosis. Porcine preantral follicles were manually dissected and cultured for up to 8 d in Waymouth’s (Exp. 1) or -minimum Eagle’s essential medium (Exp. 2 and 3) supplemented with 10 µg/mL of transferrin, 100 µg/mL of L-ascorbic acid, and 2 mU/mL of ovine FSH, in the presence (Exp. 1 and 3) or absence (Exp. 2) of 7.5% fetal calf serum. According to the experimental protocol, IGF-I (0, 1, 10, or 100 ng/mL; Exp. 1), or IGF-I (50 ng/mL), EGF (10 ng/mL) and EGF+IGF-I (Exp. 2 and 3) were added to the culture media. In Exp. 1, follicles exhibited a concentration-dependent response (P < 0.05) to IGF-I, with the highest rates of granulosal cell proliferation, follicular integrity, and recovery rate of cumulus cell-oocyte complexes and lowest incidence of apoptosis occurring at the highest IGF-I dose. In Exp. 2 serum-free medium, granulosal cell proliferation was low (1 to 5%), irrespective of whether EGF and/or IGF-I were present and cellular apoptosis was increased (P < 0.05) on d 4 and 8 in the EGF+IGF-I group compared with the addition of either factor alone. In Exp. 3, granulosal cell proliferation was high in all follicles cultured in serum-containing medium for the first 3 d, but fell sharply (P < 0.05) on d 4, except in media containing IGF-I. Collectively, EGF and IGF-I increased granulosal cell proliferation, decreased apoptosis, and promoted follicular antrum formation. These results may provide useful information for developing a preantral follicular culture system in which the oocytes are capable of fertilization and embryonic development.

Key Words: Apoptosis • Cell Proliferation • Epidermal Growth Factor • Insulin-Like Growth Factor I • In Vitro • Preantral Follicle

This article has been cited by other articles:

				K.N. Fru, M. Cherian-Shaw, M. Puttabyatappa, C.A. VandeVoort, and C.L. Chaffin Regulation of granulosa cell proliferation and EGF-like ligands during the periovulatory interval in monkeys Hum. Reprod., May 1, 2007; 22(5): 1247 - 1252. [Abstract] [Full Text] [PDF]

				L. Wen, J. Craig, P. W Dyce, and J. Li Cloning of porcine signal transducer and activator of transcription 3 cDNA and its expression in reproductive tissues. Reproduction, September 1, 2006; 132(3): 511 - 518. [Abstract] [Full Text] [PDF]

				A Higuchi, S Shimmura, T Takeuchi, M Suematsu, and K Tsubota Elucidation of apoptosis induced by serum deprivation in cultured conjunctival epithelial cells Br. J. Ophthalmol., June 1, 2006; 90(6): 760 - 764. [Abstract] [Full Text] [PDF]