HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Johnston, S. L. Articles by Olcott, B. M. Search for Related Content PubMed PubMed Citation Articles by Johnston, S. L. Articles by Olcott, B. M.

Effect of phytase addition and dietary calcium and phosphorus levels on plasma metabolites and ileal and total-tract nutrient digestibility in pigs^1,2,3,4

S. L. Johnston^*, S. B. Williams^*, L. L. Southern^*,5, T. D. Bidner^*, L. D. Bunting^*,6, J. O. Matthews^* and B. M. Olcott

^* Department of Animal Sciences, Louisiana State University Agricultural Center, Baton Rouge 70803 and and Department of Veterinary Clinical Sciences, Louisiana State UniversitySchool of Veterinary Medicine, Baton Rouge 70803

⁵ Correspondence—e-mail: lsouthern{at}agctr.lsu.edu.

Abstract

Two experiments were conducted to determine the effect of phytase on plasma metabolites and AA and energy digestibility in swine. In Exp. 1, eight barrows (surgery BW = 52 kg) were fitted with steered ileocecal cannulas. The experiment was a Latin rectangle and the treatments were 1) corn-soybean meal diet adequate in Ca and P (0.5% Ca, 0.19% available P [aP]), 2) corn-soybean meal diet with reduced Ca and P (0.4% Ca, 0.09% aP), 3) Diet 1 with 500 phytase units/kg, or 4) Diet 2 with 500 phytase units/kg. Pigs were fed twice daily to a total daily energy intake of 2.6 x maintenance (106 kcal of ME/kg of BW^0.75). For each ileal digesta sample, digesta samples were collected for two 24-h periods and combined for each pig. The combination of supplementing with phytase and decreasing the concentration of dietary Ca and P increased average ileal AA (P < 0.02), starch (P < 0.02), GE (P < 0.04), and DM (P < 0.03) digestibilities. In Exp. 2, a feeding challenge was conducted with barrows (eight per treatment; average BW of 53 kg). The treatments consisted of a corn-soybean meal diet or corn-soybean meal diet + 500 phytase units per kilogram of diet. In the diet with no phytase, Ca and aP were at 0.50% and 0.19%, respectively, and, in the diet with phytase, Ca and aP were each decreased by 0.12%. A catheter was surgically inserted into the anterior vena cava of each pig 6 d before the start of the feeding challenge. The barrows were penned individually, and the diets were fed for 3 d before the challenge. The pigs were held without feed for 16 h, and blood samples were obtained at -60, -30, and 0 min before the pigs were fed (2% of BW). Blood samples were then collected at 10, 20, 30, 40, 50, 60, 75, 90, 105, 120, 150, 180, 210, 240, 270, and 300 min after feeding. Glucose area under the response curve and plasma glucose, insulin, urea N, and total -amino N concentrations were increased (P < 0.05) in pigs fed the diet with reduced Ca and P and the phytase addition. Area under the response curve for insulin, urea N, and total -amino N; insulin:glucose; and plasma NEFA concentration, clearance, and half-life were not affected by diet. In conclusion, the combination of Ca and P reduction and phytase addition increased nutrient and energy digestibility in diets for pigs and increased plasma concentrations of glucose, insulin, urea N, and -amino N.

Key Words: Amino Acids • Digestibility • Energy • Phytase • Pigs

This article has been cited by other articles:

				C. Pomar, F. Gagne, J. J. Matte, G. Barnett, and C. Jondreville The effect of microbial phytase on true and apparent ileal amino acid digestibilities in growing-finishing pigs J Anim Sci, July 1, 2008; 86(7): 1598 - 1608. [Abstract] [Full Text] [PDF]

				T. L. Veum and M. R. Ellersieck Effect of low doses of Aspergillus niger phytase on growth performance, bone strength, and nutrient absorption and excretion by growing and finishing swine fed corn-soybean meal diets deficient in available phosphorus and calcium J Anim Sci, April 1, 2008; 86(4): 858 - 870. [Abstract] [Full Text] [PDF]

				T. A. Woyengo, J. S. Sands, W. Guenter, and C. M. Nyachoti Nutrient digestibility and performance responses of growing pigs fed phytase- and xylanase-supplemented wheat-based diets J Anim Sci, April 1, 2008; 86(4): 848 - 857. [Abstract] [Full Text] [PDF]

				E. K. D. Nyannor, P. Williams, M. R. Bedford, and O. Adeola Corn expressing an Escherichia coli-derived phytase gene: A proof-of-concept nutritional study in pigs J Anim Sci, August 1, 2007; 85(8): 1946 - 1952. [Abstract] [Full Text] [PDF]

				T. N. Nortey, J. F. Patience, P. H. Simmins, N. L. Trottier, and R. T. Zijlstra Effects of individual or combined xylanase and phytase supplementation on energy, amino acid, and phosphorus digestibility and growth performance of grower pigs fed wheat-based diets containing wheat millrun J Anim Sci, June 1, 2007; 85(6): 1432 - 1443. [Abstract] [Full Text] [PDF]

				T. L. Veum, D. R. Ledoux, and V. Raboy Low-phytate barley cultivars improve the utilization of phosphorus, calcium, nitrogen, energy, and dry matter in diets fed to young swine J Anim Sci, April 1, 2007; 85(4): 961 - 971. [Abstract] [Full Text] [PDF]

				J. A. Jendza, R. N. Dilger, J. S. Sands, and O. Adeola Efficacy and equivalency of an Escherichia coli-derived phytase for replacing inorganic phosphorus in the diets of broiler chickens and young pigs J Anim Sci, December 1, 2006; 84(12): 3364 - 3374. [Abstract] [Full Text] [PDF]

				D. V. Brana, M. Ellis, E. O. Castaneda, J. S. Sands, and D. H. Baker Effect of a novel phytase on growth performance, bone ash, and mineral digestibility in nursery and grower-finisher pigs J Anim Sci, July 1, 2006; 84(7): 1839 - 1849. [Abstract] [Full Text] [PDF]

				J. S. Radcliffe, R. S. Pleasant, and E. T. Kornegay Estimating equivalency values of microbial phytase for amino acids in growing and finishing pigs fitted with steered ileo-cecal valve cannulas J Anim Sci, May 1, 2006; 84(5): 1119 - 1129. [Abstract] [Full Text] [PDF]

				T. L. Veum, D. W. Bollinger, C. E. Buff, and M. R. Bedford A genetically engineered Escherichia coli phytase improves nutrient utilization, growth performance, and bone strength of young swine fed diets deficient in available phosphorus J Anim Sci, May 1, 2006; 84(5): 1147 - 1158. [Abstract] [Full Text] [PDF]

				J. A. Jendza, R. N. Dilger, S. A. Adedokun, J. S. Sands, and O. Adeola Escherichia coli phytase improves growth performance of starter, grower, and finisher pigs fed phosphorus-deficient diets J Anim Sci, August 1, 2005; 83(8): 1882 - 1889. [Abstract] [Full Text] [PDF]

				A. K. Kies, W. J. J. Gerrits, J. W. Schrama, M. J. W. Heetkamp, K. L. van der Linden, T. Zandstra, and M. W. A. Verstegen Mineral Absorption and Excretion as Affected by Microbial Phytase, and their Effect on Energy Metabolism in Young Piglets J. Nutr., May 1, 2005; 135(5): 1131 - 1138. [Abstract] [Full Text] [PDF]

				S. F. Liao, W. C. Sauer, A. K. Kies, Y. C. Zhang, M. Cervantes, and J. M. He Effect of phytase supplementation to diets for weanling pigs on the digestibilities of crude protein, amino acids, and energy J Anim Sci, March 1, 2005; 83(3): 625 - 633. [Abstract] [Full Text] [PDF]

				J. L. Shelton, L. L. Southern, F. M. LeMieux, T. D. Bidner, and T. G. Page Effects of microbial phytase, low calcium and phosphorus, and removing the dietary trace mineral premix on carcass traits, pork quality, plasma metabolites, and tissue mineral content in growing-finishing pigs J Anim Sci, September 1, 2004; 82(9): 2630 - 2639. [Abstract] [Full Text] [PDF]

				J. L. Shelton, J. O. Matthews, L. L. Southern, A. D. Higbie, T. D. Bidner, J. M. Fernandez, and J. E. Pontif Effect of nonwaxy and waxy sorghum on growth, carcass traits, and glucose and insulin kinetics of growing-finishing barrows and gilts J Anim Sci, June 1, 2004; 82(6): 1699 - 1706. [Abstract] [Full Text] [PDF]