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Development of fetuses from in vitro–produced and cloned bovine embryos¹

C. E. Farin^*,2, P. W. Farin and J. A. Piedrahita

^* Department of Animal Science, and Department of Population Health and Pathobiology, and and Department of Molecular Biomedical Sciences, North Carolina State University, Raleigh 27695-7621

² Correspondence: Box 7621, 231B Polk Hall (phone: 919-515-4022; fax: 919-515-7780; e-mail: char_farin{at}ncsu.edu).

The establishment of in vitro fertilization and culture systems for mammalian embryos has facilitated the application of embryo technologies in research, industry, and clinical settings. Furthermore, the advent of cloning by nuclear transfer has significantly enhanced the potential for genetic modification of livestock. Based on studies in cattle, sheep, and mice, it has become apparent that embryos produced using these systems can differ in morphology and developmental potential compared with embryos produced in vivo. Referred to as "large offspring syndrome," these abnormalities in the development of fetuses, placentas, and offspring are particularly evident following transfer of cloned embryos, but they also occur in pregnancies from embryos produced using in vitro culture alone. The objective of this review is to examine the effects of in vitro production and cloning on bovine embryo and fetal development. Literature pertaining to preimplantation embryo, conceptus, and fetal development, as well as gene expression occurring at each of these three stages, is reviewed. Physiologic and genetic mechanisms that contribute to large offspring syndrome also are discussed.

Key Words: Bovine • Cloning • Fetus • In Vitro Embryo Production • Insulin-like Growth Factors

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