J. Anim Sci.
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J. Anim. Sci. 2004. 82:3493-3499
© 2004 American Society of Animal Science

ANIMAL GROWTH, PHYSIOLOGY, AND REPRODUCTION

Effect of heat exposure on uncoupling protein-3 mRNA abundance in porcine skeletal muscle

M. Katsumata1, M. Matsumoto, S. Kawakami2 and Y. Kaji

Department of Animal and Grassland Research, National Agriculture Research Center for Kyushu Okinawa Region, Kumamoto 861-1192, Japan

1 Correspondence: Dept. of Anim. Physiol. and Nutr., Natl. Inst. of Livestock and Grassland Sci., Ibaraki, 305-0901, Japan (e-mail: masaya{at}affrc.go.jp).

Exposure to cold increases abundance of mRNA for uncoupling protein-3 (UCP3) in skeletal muscle, whereas the influence of exposure to heat is unknown. Thus, we conducted a study to investigate the influence of heat exposure on UCP3 mRNA abundance in porcine skeletal muscle. Three pigs aged 110 to 120 d, with an average BW of 75 kg, from each of eight litters were used. Each littermate was assigned to one of three treatment groups; one group was reared at 32°C and fed ad libitum (32AL) for 4 wk, whereas the other two groups were maintained at 23°C for the same period, and either pair-fed the intake of their 32AL littermates (23PF), or fed ad libitum (23AL). The RNase protection assay revealed that UCP3 mRNA abundance in longissimus dorsi and rhomboideus muscles was higher (P < 0.05) in the 32AL group than the 23PF group. The 23AL group also had significantly higher UCP3 mRNA abundance than the 23PF group in these muscles. Plasma total 3,5,3'-triiodothyronine concentration of the 32AL group was lower (P < 0.05) than that of the 23PF group, whereas mRNA abundance of thyroid hormone receptor (TR) isoforms, TR{alpha}1 and TR{alpha}2, in these muscles was not affected, suggesting that the 32AL group was in a relatively hypo-thyroid state. Because thyroid hormone up-regulates UCP3 expression, these results indicate that factors other than thyroid hormone may play a role in regulating UCP3 mRNA abundance in skeletal muscle of heat-exposed pigs.

Key Words: Uncoupling Protein-3 • 3,5,3'-Triiodothyronine • Thyroid Hormone Receptor • Heat Exposure • Skeletal Muscle • Pig






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