J. Anim Sci.
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J. Anim. Sci. 2003. 81:830-835
© 2003 American Society of Animal Science

Synchronization of Bos indicus x Bos taurus cows for timed artificial insemination using gonadotropin-releasing hormone plus prostaglandin F2{alpha} in combination with melengestrol acetate1,2

E. A. Hiers*, C. R. Barthle*, MK. V. Dahms*, G. E. Portillo*, G. A. Bridges*, D. O. Rae{dagger}, W. W. Thatcher* and J. V. Yelich*,3

* Department of Animal Sciences and and {dagger} College of Veterinary Medicine, University of Florida, Gainesville, 32611

3 Correspondence:
phone 352-392-7560; fax 352-392-7652; E-mail:
yelich{at}animal.ufl.edu.

Nonlactating Bos indicus x Bos taurus cows were used in three herds to determine the efficacy of different PGF2{alpha} treatments in combination with GnRH and melengestrol acetate (MGA) for a timed artificial insemination protocol. The start of the experiment was designated as d 0, at which time cows were assigned a body condition score and received 100 µg of GnRH. Cows were fed MGA (0.5•mg•cow-1•d-1) on d 1 to 7. On d 7, cows received either a single injection of PGF2{alpha} (Lutalyse sterile solution; 25 mg; n = 297), a single injection of cloprostenol sodium (Estrumate; 500 µg; n = 297), or half the recommended dose of PGF2{alpha} (12.5 mg; n = 275) on d 7 and 8. On d 10, all cows were artificially inseminated and received 100 µg of GnRH. Pregnancy rates to the timed artificial insemination (39%) were not affected by treatment, herd, or treatment x herd. There was an effect (P < 0.01) of artificial insemination sire on timed artificial insemination pregnancy rate for one herd, but not the other two herds. Herd influenced (P < 0.05) 30-d pregnancy rates, but there were no treatment or treatment x herd effects as 72.3% of the cows became pregnant during the first 30 d of the breeding season. Results indicate that the type of PGF2{alpha} treatment administered 7 d after GnRH did not influence timed artificial insemination pregnancy rates in nonlactating Bos indicus x Bos taurus cows.

Key Words: Artificial Insemination • Bos indicus • Gonadotropin-Releasing Hormone • Synchronization




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