J. Anim Sci.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 QUICK SEARCH:   [advanced]


     


This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Augspurger, N. R.
Right arrow Articles by Baker, D. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Augspurger, N. R.
Right arrow Articles by Baker, D. H.
J. Anim. Sci. 2003. 81:474-483
© 2003 American Society of Animal Science

Efficacy of an E. coli phytase expressed in yeast for releasing phytate-bound phosphorus in young chicks and pigs1

N. R. Augspurger*, D. M. Webel{dagger}, X. G. Lei{ddagger} and D. H. Baker*,2

* Department of Animal Sciences, University of Illinois-Urbana 61801; and {dagger} United Feeds, Inc., Sheridan, IN 46069; and and {ddagger} Department of Animal Science, Cornell University, Ithaca, NY 14853

2 Correspondence:
E-mail:
dhbaker{at}uiuc.edu.

Four chick trials and one pig trial were conducted to investigate the phosphorus-releasing efficacy of two commercial phytase enzymes (Natuphos and Ronozyme) and an experimental E. coli phytase enzyme (ECP) when added to corn-soybean meal diets containing no supplemental inorganic P (iP). In the 13- or 14-d chick trials, three or four graded levels of iP (0, 0.05, 0.10, 0.15%) from KH2PO4 were added to the basal diet to construct standard curves from which bioavailable P release could be calculated for the phytase treatments. In all cases, phytase supplementation levels were based on an assessment of phytase premix activity (i.e., P release from Na phytate at pH 5.5). Linear (P < 0.01) responses in tibia ash and weight gain resulted from iP supplementation in all assays. In the first chick trial, supplementation of 500 phytase units (FTU)/kg of ECP resulted in superior (P < 0.01) weight gain and tibia ash values compared with 500 FTU/kg of Natuphos. Results of the second chick trial revealed P-release values of 0.032 and 0.028% for 500 FTU/kg Natuphos and Ronozyme, respectively, and these were lower (P < 0.01) than the 0.125% P-release value for 500 FTU/kg of ECP. Tibia ash responded quadratically (P < 0.05) in response to graded levels of ECP up to 1,500 FTU/kg in the third chick trial. Combining Natuphos with either Ronozyme or ECP in Chick Trial 4 revealed no synergism between phytases with different initiation sites of P removal. The pig trial involved 10 individually fed weanling pigs per diet, and all phytase enzymes were supplemented to provide 400 FTU/kg in diets containing 0.60% Ca. Based on the linear regression of fibula ash on supplemental iP intake (r2 = 0.87), P-release values were 0.081% for Natuphos, 0.043% for Ronozyme, and 0.108% for ECP. These trials revealed an advantage of the E. coli phytase over the commercial phytases in young chicks.

Key Words: ChicksE. • Phytase • Pigs




This article has been cited by other articles:


Home page
J ANIM SCIHome page
T. L. Veum and M. R. Ellersieck
Effect of low doses of Aspergillus niger phytase on growth performance, bone strength, and nutrient absorption and excretion by growing and finishing swine fed corn-soybean meal diets deficient in available phosphorus and calcium
J Anim Sci, April 1, 2008; 86(4): 858 - 870.
[Abstract] [Full Text] [PDF]


Home page
Poult. Sci.Home page
N. Liu, G. H. Liu, F. D. Li, J. S. Sands, S. Zhang, A. J. Zheng, and Y. J. Ru
Efficacy of Phytases on Egg Production and Nutrient Digestibility in Layers Fed Reduced Phosphorus Diets
Poult. Sci., November 1, 2007; 86(11): 2337 - 2342.
[Abstract] [Full Text] [PDF]


Home page
J. Nutr.Home page
A. R. Pagano, K. Yasuda, K. R. Roneker, T. D. Crenshaw, and X. G. Lei
Supplemental Escherichia coli Phytase and Strontium Enhance Bone Strength of Young Pigs Fed a Phosphorus-Adequate Diet
J. Nutr., July 1, 2007; 137(7): 1795 - 1801.
[Abstract] [Full Text] [PDF]


Home page
J ANIM SCIHome page
A. R. Pagano, K. R. Roneker, and X. G. Lei
Distribution of supplemental Escherichia coli AppA2 phytase activity in digesta of various gastrointestinal segments of young pigs
J Anim Sci, June 1, 2007; 85(6): 1444 - 1452.
[Abstract] [Full Text] [PDF]


Home page
J ANIM SCIHome page
N. R. Augspurger, D. M. Webel, and D. H. Baker
An Escherichia coli phytase expressed in yeast effectively replaces inorganic phosphorus for finishing pigs and laying hens
J Anim Sci, May 1, 2007; 85(5): 1192 - 1198.
[Abstract] [Full Text] [PDF]


Home page
J ANIM SCIHome page
J. A. Jendza, R. N. Dilger, J. S. Sands, and O. Adeola
Efficacy and equivalency of an Escherichia coli-derived phytase for replacing inorganic phosphorus in the diets of broiler chickens and young pigs
J Anim Sci, December 1, 2006; 84(12): 3364 - 3374.
[Abstract] [Full Text] [PDF]


Home page
Poult. Sci.Home page
P. B. Pillai, T. O'Connor-Dennie, C. M. Owens, and J. L. Emmert
Efficacy of an Escherichia coli Phytase in Broilers Fed Adequate or Reduced Phosphorus Diets and Its Effect on Carcass Characteristics.
Poult. Sci., October 1, 2006; 85(10): 1737 - 1745.
[Abstract] [Full Text] [PDF]


Home page
Poult. Sci.Home page
A. J. Cowieson, T. Acamovic, and M. R. Bedford
Supplementation of Corn-Soy-Based Diets with an Eschericia coli-Derived Phytase: Effects on Broiler Chick Performance and the Digestibility of Amino Acids and Metabolizability of Minerals and Energy
Poult. Sci., August 1, 2006; 85(8): 1389 - 1397.
[Abstract] [Full Text] [PDF]


Home page
J ANIM SCIHome page
D. V. Brana, M. Ellis, E. O. Castaneda, J. S. Sands, and D. H. Baker
Effect of a novel phytase on growth performance, bone ash, and mineral digestibility in nursery and grower-finisher pigs
J Anim Sci, July 1, 2006; 84(7): 1839 - 1849.
[Abstract] [Full Text] [PDF]


Home page
Appl. Environ. Microbiol.Home page
T. Kim, E. J. Mullaney, J. M. Porres, K. R. Roneker, S. Crowe, S. Rice, T. Ko, A. H. J. Ullah, C. B. Daly, R. Welch, et al.
Shifting the pH Profile of Aspergillus niger PhyA Phytase To Match the Stomach pH Enhances Its Effectiveness as an Animal Feed Additive.
Appl. Envir. Microbiol., June 1, 2006; 72(6): 4397 - 4403.
[Abstract] [Full Text] [PDF]


Home page
J ANIM SCIHome page
T. L. Veum, D. W. Bollinger, C. E. Buff, and M. R. Bedford
A genetically engineered Escherichia coli phytase improves nutrient utilization, growth performance, and bone strength of young swine fed diets deficient in available phosphorus
J Anim Sci, May 1, 2006; 84(5): 1147 - 1158.
[Abstract] [Full Text] [PDF]


Home page
J ANIM SCIHome page
J. A. Jendza, R. N. Dilger, S. A. Adedokun, J. S. Sands, and O. Adeola
Escherichia coli phytase improves growth performance of starter, grower, and finisher pigs fed phosphorus-deficient diets
J Anim Sci, August 1, 2005; 83(8): 1882 - 1889.
[Abstract] [Full Text] [PDF]


Home page
J ANIM SCIHome page
T. W. Kim and X. G. Lei
An improved method for a rapid determination of phytase activity in animal feed
J Anim Sci, May 1, 2005; 83(5): 1062 - 1067.
[Abstract] [Full Text] [PDF]


Home page
J ANIM SCIHome page
O. Adeola, J. S. Sands, P. H. Simmins, and H. Schulze
The efficacy of an Escherichia coli-derived phytase preparation
J Anim Sci, September 1, 2004; 82(9): 2657 - 2666.
[Abstract] [Full Text] [PDF]


Home page
J ANIM SCIHome page
C. H. Stahl, K. R. Roneker, W. G. Pond, and X. G. Lei
Effects of combining three fungal phytases with a bacterial phytase on plasma phosphorus status of weanling pigs fed a corn-soy diet
J Anim Sci, June 1, 2004; 82(6): 1725 - 1731.
[Abstract] [Full Text] [PDF]


Home page
J ANIM SCIHome page
N. R. Augspurger, J. D. Spencer, D. M. Webel, and D. H. Baker
Pharmacological zinc levels reduce the phosphorus-releasing efficacy of phytase in young pigs and chickens
J Anim Sci, June 1, 2004; 82(6): 1732 - 1739.
[Abstract] [Full Text] [PDF]


Home page
J ANIM SCIHome page
N. R. Augspurger and D. H. Baker
High dietary phytase levels maximize phytate-phosphorus utilization but do not affect protein utilization in chicks fed phosphorus- or amino acid-deficient diets
J Anim Sci, April 1, 2004; 82(4): 1100 - 1107.
[Abstract] [Full Text] [PDF]


Home page
J ANIM SCIHome page
J. M. Gentile, K. R. Roneker, S. E. Crowe, W. G. Pond, and X. G. Lei
Effectiveness of an experimental consensus phytase in improving dietary phytate-phosphorus utilization by weanling pigs
J Anim Sci, November 1, 2003; 81(11): 2751 - 2757.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2003 by the American Society of Animal Science.