|
|
||||||||


* Department of Animal Sciences, University of Illinois-Urbana 61801;
and
United Feeds, Inc., Sheridan, IN 46069; and
and
Department of Animal Science, Cornell University, Ithaca, NY 14853
2 Correspondence:
E-mail:
dhbaker{at}uiuc.edu.
Four chick trials and one pig trial were conducted to investigate the phosphorus-releasing efficacy of two commercial phytase enzymes (Natuphos and Ronozyme) and an experimental E. coli phytase enzyme (ECP) when added to corn-soybean meal diets containing no supplemental inorganic P (iP). In the 13- or 14-d chick trials, three or four graded levels of iP (0, 0.05, 0.10, 0.15%) from KH2PO4 were added to the basal diet to construct standard curves from which bioavailable P release could be calculated for the phytase treatments. In all cases, phytase supplementation levels were based on an assessment of phytase premix activity (i.e., P release from Na phytate at pH 5.5). Linear (P < 0.01) responses in tibia ash and weight gain resulted from iP supplementation in all assays. In the first chick trial, supplementation of 500 phytase units (FTU)/kg of ECP resulted in superior (P < 0.01) weight gain and tibia ash values compared with 500 FTU/kg of Natuphos. Results of the second chick trial revealed P-release values of 0.032 and 0.028% for 500 FTU/kg Natuphos and Ronozyme, respectively, and these were lower (P < 0.01) than the 0.125% P-release value for 500 FTU/kg of ECP. Tibia ash responded quadratically (P < 0.05) in response to graded levels of ECP up to 1,500 FTU/kg in the third chick trial. Combining Natuphos with either Ronozyme or ECP in Chick Trial 4 revealed no synergism between phytases with different initiation sites of P removal. The pig trial involved 10 individually fed weanling pigs per diet, and all phytase enzymes were supplemented to provide 400 FTU/kg in diets containing 0.60% Ca. Based on the linear regression of fibula ash on supplemental iP intake (r2 = 0.87), P-release values were 0.081% for Natuphos, 0.043% for Ronozyme, and 0.108% for ECP. These trials revealed an advantage of the E. coli phytase over the commercial phytases in young chicks.
Key Words: ChicksE. Phytase Pigs
This article has been cited by other articles:
![]() |
T. L. Veum and M. R. Ellersieck Effect of low doses of Aspergillus niger phytase on growth performance, bone strength, and nutrient absorption and excretion by growing and finishing swine fed corn-soybean meal diets deficient in available phosphorus and calcium J Anim Sci, April 1, 2008; 86(4): 858 - 870. [Abstract] [Full Text] [PDF] |
||||
![]() |
N. Liu, G. H. Liu, F. D. Li, J. S. Sands, S. Zhang, A. J. Zheng, and Y. J. Ru Efficacy of Phytases on Egg Production and Nutrient Digestibility in Layers Fed Reduced Phosphorus Diets Poult. Sci., November 1, 2007; 86(11): 2337 - 2342. [Abstract] [Full Text] [PDF] |
||||
![]() |
A. R. Pagano, K. Yasuda, K. R. Roneker, T. D. Crenshaw, and X. G. Lei Supplemental Escherichia coli Phytase and Strontium Enhance Bone Strength of Young Pigs Fed a Phosphorus-Adequate Diet J. Nutr., July 1, 2007; 137(7): 1795 - 1801. [Abstract] [Full Text] [PDF] |
||||
![]() |
A. R. Pagano, K. R. Roneker, and X. G. Lei Distribution of supplemental Escherichia coli AppA2 phytase activity in digesta of various gastrointestinal segments of young pigs J Anim Sci, June 1, 2007; 85(6): 1444 - 1452. [Abstract] [Full Text] [PDF] |
||||
![]() |
N. R. Augspurger, D. M. Webel, and D. H. Baker An Escherichia coli phytase expressed in yeast effectively replaces inorganic phosphorus for finishing pigs and laying hens J Anim Sci, May 1, 2007; 85(5): 1192 - 1198. [Abstract] [Full Text] [PDF] |
||||
![]() |
J. A. Jendza, R. N. Dilger, J. S. Sands, and O. Adeola Efficacy and equivalency of an Escherichia coli-derived phytase for replacing inorganic phosphorus in the diets of broiler chickens and young pigs J Anim Sci, December 1, 2006; 84(12): 3364 - 3374. [Abstract] [Full Text] [PDF] |
||||
![]() |
P. B. Pillai, T. O'Connor-Dennie, C. M. Owens, and J. L. Emmert Efficacy of an Escherichia coli Phytase in Broilers Fed Adequate or Reduced Phosphorus Diets and Its Effect on Carcass Characteristics. Poult. Sci., October 1, 2006; 85(10): 1737 - 1745. [Abstract] [Full Text] [PDF] |
||||
![]() |
A. J. Cowieson, T. Acamovic, and M. R. Bedford Supplementation of Corn-Soy-Based Diets with an Eschericia coli-Derived Phytase: Effects on Broiler Chick Performance and the Digestibility of Amino Acids and Metabolizability of Minerals and Energy Poult. Sci., August 1, 2006; 85(8): 1389 - 1397. [Abstract] [Full Text] [PDF] |
||||
![]() |
D. V. Brana, M. Ellis, E. O. Castaneda, J. S. Sands, and D. H. Baker Effect of a novel phytase on growth performance, bone ash, and mineral digestibility in nursery and grower-finisher pigs J Anim Sci, July 1, 2006; 84(7): 1839 - 1849. [Abstract] [Full Text] [PDF] |
||||
![]() |
T. Kim, E. J. Mullaney, J. M. Porres, K. R. Roneker, S. Crowe, S. Rice, T. Ko, A. H. J. Ullah, C. B. Daly, R. Welch, et al. Shifting the pH Profile of Aspergillus niger PhyA Phytase To Match the Stomach pH Enhances Its Effectiveness as an Animal Feed Additive. Appl. Envir. Microbiol., June 1, 2006; 72(6): 4397 - 4403. [Abstract] [Full Text] [PDF] |
||||
![]() |
T. L. Veum, D. W. Bollinger, C. E. Buff, and M. R. Bedford A genetically engineered Escherichia coli phytase improves nutrient utilization, growth performance, and bone strength of young swine fed diets deficient in available phosphorus J Anim Sci, May 1, 2006; 84(5): 1147 - 1158. [Abstract] [Full Text] [PDF] |
||||
![]() |
J. A. Jendza, R. N. Dilger, S. A. Adedokun, J. S. Sands, and O. Adeola Escherichia coli phytase improves growth performance of starter, grower, and finisher pigs fed phosphorus-deficient diets J Anim Sci, August 1, 2005; 83(8): 1882 - 1889. [Abstract] [Full Text] [PDF] |
||||
![]() |
T. W. Kim and X. G. Lei An improved method for a rapid determination of phytase activity in animal feed J Anim Sci, May 1, 2005; 83(5): 1062 - 1067. [Abstract] [Full Text] [PDF] |
||||
![]() |
O. Adeola, J. S. Sands, P. H. Simmins, and H. Schulze The efficacy of an Escherichia coli-derived phytase preparation J Anim Sci, September 1, 2004; 82(9): 2657 - 2666. [Abstract] [Full Text] [PDF] |
||||
![]() |
C. H. Stahl, K. R. Roneker, W. G. Pond, and X. G. Lei Effects of combining three fungal phytases with a bacterial phytase on plasma phosphorus status of weanling pigs fed a corn-soy diet J Anim Sci, June 1, 2004; 82(6): 1725 - 1731. [Abstract] [Full Text] [PDF] |
||||
![]() |
N. R. Augspurger, J. D. Spencer, D. M. Webel, and D. H. Baker Pharmacological zinc levels reduce the phosphorus-releasing efficacy of phytase in young pigs and chickens J Anim Sci, June 1, 2004; 82(6): 1732 - 1739. [Abstract] [Full Text] [PDF] |
||||
![]() |
N. R. Augspurger and D. H. Baker High dietary phytase levels maximize phytate-phosphorus utilization but do not affect protein utilization in chicks fed phosphorus- or amino acid-deficient diets J Anim Sci, April 1, 2004; 82(4): 1100 - 1107. [Abstract] [Full Text] [PDF] |
||||
![]() |
J. M. Gentile, K. R. Roneker, S. E. Crowe, W. G. Pond, and X. G. Lei Effectiveness of an experimental consensus phytase in improving dietary phytate-phosphorus utilization by weanling pigs J Anim Sci, November 1, 2003; 81(11): 2751 - 2757. [Abstract] [Full Text] [PDF] |
||||
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |