HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kushibiki, S. Articles by Yokomizo, Y. Search for Related Content PubMed PubMed Citation Articles by Kushibiki, S. Articles by Yokomizo, Y.

Alterations in lipid metabolism induced by recombinant bovine tumor necrosis factor-alpha administration to dairy heifers^1,2

S. Kushibiki^*,3, K. Hodate, H. Shingu^*, T. Hayashi, E. Touno^*, M. Shinoda^* and Y. Yokomizo^#

^* Department of Animal Production, National Agricultural Research Center for Tohoku Region, Iwate, 020-0198, Japan; and Kitasato University, Aomori, 034-8628, Japan; and National Agricultural Research Center, Ibaraki, 305-8666, Japan; and and ^# National Institute of Animal Health, Ibaraki, 305-0856, Japan

³ Correspondence:
phone: +81-19-643-3546; fax: +81-19-643-3547; E-mail:
mendoza{at}affrc.go.jp.

Endotoxin induces marked changes in lipid metabolism via its effects on cytokines. To evaluate the role of tumor necrosis factor-alpha (TNF) in mediating changes of lipid metabolism in ruminants, we performed a crossover saline-controlled study in Holstein heifers (n = 8; 394.0 kg average BW), investigating the metabolic effects of a single intravenous administration of recombinant bovine TNF (rbTNF, 5.0 µg/kg). Blood samples were taken from a jugular vein at 0 (1100, just before injection), 0.5, 6, 12, and 24 h after each treatment. Dry matter intake in the heifers was not affected by single administration of the rbTNF. The rbTNF produced early as well as later hypertriglyceridemia (P < 0.05) in dairy heifers. The rbTNF also induced an early and sustained rise (P < 0.05) in the plasma NEFA concentration. Plasma retinol concentration was decreased (P < 0.05) at 24 h after rbTNF injection, whereas the -tocopherol concentration was not significantly affected by rbTNF treatment. At 0.5 and 24 h, there was an increase (P < 0.05) in the plasma concentration of the very-low-density lipoprotein (VLDL) fraction in rbTNF-treated heifers. Between 6 and 24 h after rbTNF treatment, concentration of the low-density lipoprotein fraction declined (P < 0.05) but the high-density lipoprotein fraction was not altered in the rbTNF-treated heifers. These results indicate that TNF produces a hypertriglyceridemic response associated with an increase of the VLDL fraction and a disturbance of retinol metabolism in dairy heifers.

Key Words: Dairy Cattle • Lipoproteins • Retinol • Tumor Necrosis Factor • Vitamin E Acetate

This article has been cited by other articles:

				M. R. Waldron, A. E. Kulick, A. W. Bell, and T. R. Overton Acute Experimental Mastitis Is Not Causal Toward the Development of Energy-Related Metabolic Disorders in Early Postpartum Dairy Cows J Dairy Sci, February 1, 2006; 89(2): 596 - 610. [Abstract] [Full Text] [PDF]

				S. Kushibiki, H. Shingu, T. Komatsu, F. Itoh, T. Hayashi, and K. Hodate Plasma Lipid Concentrations in Preruminant Calves Fed Whole Milk with Whey Protein Isolate J Dairy Sci, February 1, 2005; 88(2): 653 - 659. [Abstract] [Full Text] [PDF]

				M. R. Waldron, T. Nishida, B. J. Nonnecke, and T. R. Overton Effect of Lipopolysaccharide on Indices of Peripheral and Hepatic Metabolism in Lactating Cows J Dairy Sci, November 1, 2003; 86(11): 3447 - 3459. [Abstract] [Full Text] [PDF]